Optimization of Fluorescence In Situ Hybridization technique for analyzing microorganisms involved in bioprocesses
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/17068 |
Resumo: | This work is focused on FISH technique optimization for yeast and bacteria analysis, by enhancing the FISH signals by increasing the number of fluorescent cells and their fluorescence intensities. With this aim different fixation procedures and hybridization conditions were tested. The modifications introduced in the procedure represent an important contribution for FISH optimization. |
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Optimization of Fluorescence In Situ Hybridization technique for analyzing microorganisms involved in bioprocessesFISHmicroorganismsbioprocessesThis work is focused on FISH technique optimization for yeast and bacteria analysis, by enhancing the FISH signals by increasing the number of fluorescent cells and their fluorescence intensities. With this aim different fixation procedures and hybridization conditions were tested. The modifications introduced in the procedure represent an important contribution for FISH optimization.sciBAC,scientific Congress of FEBiotec– BAC 20152016-01-29T11:51:45Z2016-01-292015-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/17068http://hdl.handle.net/10174/17068engOptimization of Fluorescence In Situ Hybridization technique for analyzing microorganisms involved in bioprocesses, Marina González-Pérez, Ricardo Vieira, Patrícia Nunes, António Pereira, António Candeias, Ana T. Caldeira, sciBAC,scientific Congress of FEBiotec– BAC 2015, Salamanca (Spain), 08/07/2015 to 10/07/2015978-84-606-9600-1http://issuu.com/febiotec/docs/libro_bac2015marinagonpe@gmail.comndndamlp@uevora.ptcandeias@uevora.ptatc@uevora.pt303Pérez, Marina GonzálezVieira, RicardoNunes, PatríciaPereira, AntónioCandeias, AntónioCaldeira, Ana T.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:04:17Zoai:dspace.uevora.pt:10174/17068Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:09:19.056144Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Optimization of Fluorescence In Situ Hybridization technique for analyzing microorganisms involved in bioprocesses |
title |
Optimization of Fluorescence In Situ Hybridization technique for analyzing microorganisms involved in bioprocesses |
spellingShingle |
Optimization of Fluorescence In Situ Hybridization technique for analyzing microorganisms involved in bioprocesses Pérez, Marina González FISH microorganisms bioprocesses |
title_short |
Optimization of Fluorescence In Situ Hybridization technique for analyzing microorganisms involved in bioprocesses |
title_full |
Optimization of Fluorescence In Situ Hybridization technique for analyzing microorganisms involved in bioprocesses |
title_fullStr |
Optimization of Fluorescence In Situ Hybridization technique for analyzing microorganisms involved in bioprocesses |
title_full_unstemmed |
Optimization of Fluorescence In Situ Hybridization technique for analyzing microorganisms involved in bioprocesses |
title_sort |
Optimization of Fluorescence In Situ Hybridization technique for analyzing microorganisms involved in bioprocesses |
author |
Pérez, Marina González |
author_facet |
Pérez, Marina González Vieira, Ricardo Nunes, Patrícia Pereira, António Candeias, António Caldeira, Ana T. |
author_role |
author |
author2 |
Vieira, Ricardo Nunes, Patrícia Pereira, António Candeias, António Caldeira, Ana T. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pérez, Marina González Vieira, Ricardo Nunes, Patrícia Pereira, António Candeias, António Caldeira, Ana T. |
dc.subject.por.fl_str_mv |
FISH microorganisms bioprocesses |
topic |
FISH microorganisms bioprocesses |
description |
This work is focused on FISH technique optimization for yeast and bacteria analysis, by enhancing the FISH signals by increasing the number of fluorescent cells and their fluorescence intensities. With this aim different fixation procedures and hybridization conditions were tested. The modifications introduced in the procedure represent an important contribution for FISH optimization. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07-01T00:00:00Z 2016-01-29T11:51:45Z 2016-01-29 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/17068 http://hdl.handle.net/10174/17068 |
url |
http://hdl.handle.net/10174/17068 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Optimization of Fluorescence In Situ Hybridization technique for analyzing microorganisms involved in bioprocesses, Marina González-Pérez, Ricardo Vieira, Patrícia Nunes, António Pereira, António Candeias, Ana T. Caldeira, sciBAC,scientific Congress of FEBiotec– BAC 2015, Salamanca (Spain), 08/07/2015 to 10/07/2015 978-84-606-9600-1 http://issuu.com/febiotec/docs/libro_bac2015 marinagonpe@gmail.com nd nd amlp@uevora.pt candeias@uevora.pt atc@uevora.pt 303 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
sciBAC,scientific Congress of FEBiotec– BAC 2015 |
publisher.none.fl_str_mv |
sciBAC,scientific Congress of FEBiotec– BAC 2015 |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799136575752241152 |