Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity, and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeira
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/3156 |
Resumo: | Three macroalgae collected at Madeira Island were included in this study to determine their potential for drug, nutraceutical, food, or supplement application. Fatty acid content was higher in Zonaria tournefortii (12.32 mg g⁻¹ dw) with 16.58% of PUFAs, eicosapentaenoic acid (C20:5 omega 3), and arachidonic acid (20:4 omega 6) having concentrations of 2.59 and 1.17%, respectively. The anti-thrombogenic and anti-atherogenicity potential was higher for Z. tournefortii due to relevant fatty acids in the biochemical composition this macroalgae. Lipid classes were assessed in the lipid extract and neutral lipids (NL) were in higher yield in Asparagopsis taxiformis (51.16%) and lower in Z. tournefortii (26.96%). The glycolipids (GL) were between 36.03 and 16.11% in Z. tournefortii and Ulva lactuca. Phospholipids (PL) fraction varied from 35.91 and 31.60% in A. taxiformis and Z. tournefortii. TLC screening identified that U. lactuca contains phytol and cholesterol in its NL, digalactosyldiacylglycerol in its GL, and cardiolipin and L-alpha-phosphatidylcholine in its PL. Zonaria tournefortii contains phytol and cholesterol in its NL classes, and the PL classes contain L-alpha-phosphatidylethanolamine and 1-(3-sn-phosphatidyl)-rac-glycerol. The macroalgae A. taxiformis revealed cholesterol in its NL fraction and the same phospholipids as Z. tournefortii in its PL fraction. ATR-FTIR analysis enabled a "fingerprint" spectra and important sulfation absorption bands were identified, revealing the functional polysaccharides within these macroalgae. Anti-cholinesterasic activity was assessed in A. taxiformis, with a low IC50 for AChE (8.92 +/- 0.43 mu g mL⁻¹) and BuChE (13.96 +/- 0.32 mu g mL⁻¹), demonstrating dual inhibitory activity, justifying the interest to identify the active principle which may be the scaffold of a novel drug. |
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Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity, and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in MadeiraSeaweedsLipidsAsparagopsis taxiformisUlva lactucaZonaria tournefortiiPUFAsMUFAsSFAsThree macroalgae collected at Madeira Island were included in this study to determine their potential for drug, nutraceutical, food, or supplement application. Fatty acid content was higher in Zonaria tournefortii (12.32 mg g⁻¹ dw) with 16.58% of PUFAs, eicosapentaenoic acid (C20:5 omega 3), and arachidonic acid (20:4 omega 6) having concentrations of 2.59 and 1.17%, respectively. The anti-thrombogenic and anti-atherogenicity potential was higher for Z. tournefortii due to relevant fatty acids in the biochemical composition this macroalgae. Lipid classes were assessed in the lipid extract and neutral lipids (NL) were in higher yield in Asparagopsis taxiformis (51.16%) and lower in Z. tournefortii (26.96%). The glycolipids (GL) were between 36.03 and 16.11% in Z. tournefortii and Ulva lactuca. Phospholipids (PL) fraction varied from 35.91 and 31.60% in A. taxiformis and Z. tournefortii. TLC screening identified that U. lactuca contains phytol and cholesterol in its NL, digalactosyldiacylglycerol in its GL, and cardiolipin and L-alpha-phosphatidylcholine in its PL. Zonaria tournefortii contains phytol and cholesterol in its NL classes, and the PL classes contain L-alpha-phosphatidylethanolamine and 1-(3-sn-phosphatidyl)-rac-glycerol. The macroalgae A. taxiformis revealed cholesterol in its NL fraction and the same phospholipids as Z. tournefortii in its PL fraction. ATR-FTIR analysis enabled a "fingerprint" spectra and important sulfation absorption bands were identified, revealing the functional polysaccharides within these macroalgae. Anti-cholinesterasic activity was assessed in A. taxiformis, with a low IC50 for AChE (8.92 +/- 0.43 mu g mL⁻¹) and BuChE (13.96 +/- 0.32 mu g mL⁻¹), demonstrating dual inhibitory activity, justifying the interest to identify the active principle which may be the scaffold of a novel drug.This work was financially supported by DemoBlueAlgae “Desenvolvimento de metodologias e optimização dos processos de cultivo e processamento de macroalgas para a indústria e economia azul” PROCiência 2020 (M1420-01-0247-FEDER000002); MACBIOBLUE “Proyecto demostrativo y de transferencia tecnológica para ayudar a las empresas a desarrollar nuevos produtos y procesos en el ambito de la Biotecnología Azul de la Macaronesia” (MAC/1.1b/ 086), program Interreg MAC 2014–2020; ARDITI - Regional Agency for the Development of Research Technology and Innovation (M14-20-09-5369-FSE-000001-Doctorate in Business; Blue Iodine II “Boost Blue economy through market uptake an innovative seaweed bioextract for iodine fortification II”, grant agreement no. 733552, H2020-SMEInst-2016-2017.SpringerRepositório da Universidade dos AçoresNunes, NunoRosa, Gonçalo P.Ferraz, SóniaBarreto, Maria do CarmoCarvalho, Miguel A. A. Pinheiro de2021-05-31T10:37:32Z2019-082019-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/3156engNunes, N., Rosa, G.P., Ferraz, S., Barreto, M.C., Pinheiro de Carvalho, M.A.A. (2020). Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeira. "Journal of Applied Phycology", 32, 759-771. DOI:10.1007/s10811-019-01884-91573-517610.1007/s10811-019-01884-9000534338500002info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-12-20T14:34:11Zoai:repositorio.uac.pt:10400.3/5948Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:28:00.171340Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity, and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeira |
title |
Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity, and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeira |
spellingShingle |
Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity, and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeira Nunes, Nuno Seaweeds Lipids Asparagopsis taxiformis Ulva lactuca Zonaria tournefortii PUFAs MUFAs SFAs |
title_short |
Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity, and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeira |
title_full |
Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity, and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeira |
title_fullStr |
Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity, and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeira |
title_full_unstemmed |
Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity, and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeira |
title_sort |
Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity, and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeira |
author |
Nunes, Nuno |
author_facet |
Nunes, Nuno Rosa, Gonçalo P. Ferraz, Sónia Barreto, Maria do Carmo Carvalho, Miguel A. A. Pinheiro de |
author_role |
author |
author2 |
Rosa, Gonçalo P. Ferraz, Sónia Barreto, Maria do Carmo Carvalho, Miguel A. A. Pinheiro de |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade dos Açores |
dc.contributor.author.fl_str_mv |
Nunes, Nuno Rosa, Gonçalo P. Ferraz, Sónia Barreto, Maria do Carmo Carvalho, Miguel A. A. Pinheiro de |
dc.subject.por.fl_str_mv |
Seaweeds Lipids Asparagopsis taxiformis Ulva lactuca Zonaria tournefortii PUFAs MUFAs SFAs |
topic |
Seaweeds Lipids Asparagopsis taxiformis Ulva lactuca Zonaria tournefortii PUFAs MUFAs SFAs |
description |
Three macroalgae collected at Madeira Island were included in this study to determine their potential for drug, nutraceutical, food, or supplement application. Fatty acid content was higher in Zonaria tournefortii (12.32 mg g⁻¹ dw) with 16.58% of PUFAs, eicosapentaenoic acid (C20:5 omega 3), and arachidonic acid (20:4 omega 6) having concentrations of 2.59 and 1.17%, respectively. The anti-thrombogenic and anti-atherogenicity potential was higher for Z. tournefortii due to relevant fatty acids in the biochemical composition this macroalgae. Lipid classes were assessed in the lipid extract and neutral lipids (NL) were in higher yield in Asparagopsis taxiformis (51.16%) and lower in Z. tournefortii (26.96%). The glycolipids (GL) were between 36.03 and 16.11% in Z. tournefortii and Ulva lactuca. Phospholipids (PL) fraction varied from 35.91 and 31.60% in A. taxiformis and Z. tournefortii. TLC screening identified that U. lactuca contains phytol and cholesterol in its NL, digalactosyldiacylglycerol in its GL, and cardiolipin and L-alpha-phosphatidylcholine in its PL. Zonaria tournefortii contains phytol and cholesterol in its NL classes, and the PL classes contain L-alpha-phosphatidylethanolamine and 1-(3-sn-phosphatidyl)-rac-glycerol. The macroalgae A. taxiformis revealed cholesterol in its NL fraction and the same phospholipids as Z. tournefortii in its PL fraction. ATR-FTIR analysis enabled a "fingerprint" spectra and important sulfation absorption bands were identified, revealing the functional polysaccharides within these macroalgae. Anti-cholinesterasic activity was assessed in A. taxiformis, with a low IC50 for AChE (8.92 +/- 0.43 mu g mL⁻¹) and BuChE (13.96 +/- 0.32 mu g mL⁻¹), demonstrating dual inhibitory activity, justifying the interest to identify the active principle which may be the scaffold of a novel drug. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08 2019-08-01T00:00:00Z 2021-05-31T10:37:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/3156 |
url |
http://hdl.handle.net/10400.13/3156 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nunes, N., Rosa, G.P., Ferraz, S., Barreto, M.C., Pinheiro de Carvalho, M.A.A. (2020). Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeira. "Journal of Applied Phycology", 32, 759-771. DOI:10.1007/s10811-019-01884-9 1573-5176 10.1007/s10811-019-01884-9 000534338500002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130737686872064 |