Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in Axons
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/106851 https://doi.org/10.1016/j.celrep.2019.06.080 |
Resumo: | Ribosomes and a subset of cellular mRNAs are trafficked into axons of developing neurons. The axonal localization of translational machinery allows new proteins to be rapidly and locally synthesized during axonal growth and pathfinding. However, in mature neurons, axonal ribosomes are significantly reduced or even absent. The mechanism that elicits this removal is currently unknown. Here, we demonstrate that synapse formation is the trigger for ribosome reduction in mature axons. In vivo analysis shows that axonal ribosome levels decrease in rat brain at a developmental stage coincident with synapse formation. Next, we observe in vitro that different synaptogenic inducers trigger an overall decrease of ribosomal proteins and rRNA in the axons of spinal motor neurons. We further observe that this process is dependent on the ubiquitin-proteasome system but not on autophagy. Together, these data identify synaptogenesis as the long missing biological trigger that leads to ribosome disappearance during axonal maturation. |
id |
RCAP_9f653ee0f01b94eb9bd76759ed1506bc |
---|---|
oai_identifier_str |
oai:estudogeral.uc.pt:10316/106851 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in AxonsUPSaxonal maturationco-culturesneuromuscular junctionpresynaptic differentiationproteasomeribosomal proteinsribosomesynapse formationsynaptogenesisAnimalsAxonsCell DifferentiationFemaleHEK293 CellsHumansMiceNeuromuscular JunctionPresynaptic TerminalsProteasome Endopeptidase ComplexRNA, RibosomalRats, Sprague-DawleyRibosomesSynapsesUbiquitinNeurogenesisRibosomes and a subset of cellular mRNAs are trafficked into axons of developing neurons. The axonal localization of translational machinery allows new proteins to be rapidly and locally synthesized during axonal growth and pathfinding. However, in mature neurons, axonal ribosomes are significantly reduced or even absent. The mechanism that elicits this removal is currently unknown. Here, we demonstrate that synapse formation is the trigger for ribosome reduction in mature axons. In vivo analysis shows that axonal ribosome levels decrease in rat brain at a developmental stage coincident with synapse formation. Next, we observe in vitro that different synaptogenic inducers trigger an overall decrease of ribosomal proteins and rRNA in the axons of spinal motor neurons. We further observe that this process is dependent on the ubiquitin-proteasome system but not on autophagy. Together, these data identify synaptogenesis as the long missing biological trigger that leads to ribosome disappearance during axonal maturation.Elsevier2019-07-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/106851http://hdl.handle.net/10316/106851https://doi.org/10.1016/j.celrep.2019.06.080eng22111247Costa, Rui O.Martins, HelenaMartins, Luís F.Cwetsch, Andrzej WMele, MirandaPedro, Joana R.Tomé, DiogoJeon, Noo LiCancedda, LauraJaffrey, Samie R.Almeida, Ramiro D.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-04-27T08:59:32Zoai:estudogeral.uc.pt:10316/106851Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:23:14.916719Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in Axons |
title |
Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in Axons |
spellingShingle |
Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in Axons Costa, Rui O. UPS axonal maturation co-cultures neuromuscular junction presynaptic differentiation proteasome ribosomal proteins ribosome synapse formation synaptogenesis Animals Axons Cell Differentiation Female HEK293 Cells Humans Mice Neuromuscular Junction Presynaptic Terminals Proteasome Endopeptidase Complex RNA, Ribosomal Rats, Sprague-Dawley Ribosomes Synapses Ubiquitin Neurogenesis |
title_short |
Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in Axons |
title_full |
Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in Axons |
title_fullStr |
Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in Axons |
title_full_unstemmed |
Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in Axons |
title_sort |
Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in Axons |
author |
Costa, Rui O. |
author_facet |
Costa, Rui O. Martins, Helena Martins, Luís F. Cwetsch, Andrzej W Mele, Miranda Pedro, Joana R. Tomé, Diogo Jeon, Noo Li Cancedda, Laura Jaffrey, Samie R. Almeida, Ramiro D. |
author_role |
author |
author2 |
Martins, Helena Martins, Luís F. Cwetsch, Andrzej W Mele, Miranda Pedro, Joana R. Tomé, Diogo Jeon, Noo Li Cancedda, Laura Jaffrey, Samie R. Almeida, Ramiro D. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Costa, Rui O. Martins, Helena Martins, Luís F. Cwetsch, Andrzej W Mele, Miranda Pedro, Joana R. Tomé, Diogo Jeon, Noo Li Cancedda, Laura Jaffrey, Samie R. Almeida, Ramiro D. |
dc.subject.por.fl_str_mv |
UPS axonal maturation co-cultures neuromuscular junction presynaptic differentiation proteasome ribosomal proteins ribosome synapse formation synaptogenesis Animals Axons Cell Differentiation Female HEK293 Cells Humans Mice Neuromuscular Junction Presynaptic Terminals Proteasome Endopeptidase Complex RNA, Ribosomal Rats, Sprague-Dawley Ribosomes Synapses Ubiquitin Neurogenesis |
topic |
UPS axonal maturation co-cultures neuromuscular junction presynaptic differentiation proteasome ribosomal proteins ribosome synapse formation synaptogenesis Animals Axons Cell Differentiation Female HEK293 Cells Humans Mice Neuromuscular Junction Presynaptic Terminals Proteasome Endopeptidase Complex RNA, Ribosomal Rats, Sprague-Dawley Ribosomes Synapses Ubiquitin Neurogenesis |
description |
Ribosomes and a subset of cellular mRNAs are trafficked into axons of developing neurons. The axonal localization of translational machinery allows new proteins to be rapidly and locally synthesized during axonal growth and pathfinding. However, in mature neurons, axonal ribosomes are significantly reduced or even absent. The mechanism that elicits this removal is currently unknown. Here, we demonstrate that synapse formation is the trigger for ribosome reduction in mature axons. In vivo analysis shows that axonal ribosome levels decrease in rat brain at a developmental stage coincident with synapse formation. Next, we observe in vitro that different synaptogenic inducers trigger an overall decrease of ribosomal proteins and rRNA in the axons of spinal motor neurons. We further observe that this process is dependent on the ubiquitin-proteasome system but not on autophagy. Together, these data identify synaptogenesis as the long missing biological trigger that leads to ribosome disappearance during axonal maturation. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07-23 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/106851 http://hdl.handle.net/10316/106851 https://doi.org/10.1016/j.celrep.2019.06.080 |
url |
http://hdl.handle.net/10316/106851 https://doi.org/10.1016/j.celrep.2019.06.080 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
22111247 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799134120163409920 |