Biological characterization of Bothrops marajoensis snake venom
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://libpubmedia.co.uk/biological-characterization-of-bothrops-marajoensis-snake-venom/ http://hdl.handle.net/11449/141050 |
Resumo: | This study describes the effects of Bothrops marajoensis venom (Marajó lancehead) on isolated neuromuscular preparations of chick biventer cervicis (CBC) and mouse phrenic nerve-diaphragm (PND). At low concentrations (1µg/ml for CBC and 5µg/ml for PND), the venom exhibited a neuromuscular blocking without any damaging effect on the muscle integrity. At higher concentration (20µg/ml for PND), together with the neuromuscular blockade, there was a moderate myonecrosis. The results show differences between mammalian and avian preparations in response to venom concentration; the avian preparation was more sensitive to venom neurotoxic effect than the mammalian preparation. The possible presynaptic mechanism underlying the neuromuscular blocking effect was reinforced by the observed increase in MEPPs at the same time (at 15min) when the facilitation of twitch tension occurred. These results indicate that the B. marajoensis venom produced neuromuscular blockade, which appeared to be presynaptic at low concentrations with a postsynaptic component at high concentrations, leading to muscle oedema. These observations demand the fractionation of the crude venom and characterization of its active components for a better understanding of its biological dynamics. |
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Biological characterization of Bothrops marajoensis snake venomMarajó lanceheadNeuromuscular junctionNeurotoxicityMyotoxicityPresynaptic effectsThis study describes the effects of Bothrops marajoensis venom (Marajó lancehead) on isolated neuromuscular preparations of chick biventer cervicis (CBC) and mouse phrenic nerve-diaphragm (PND). At low concentrations (1µg/ml for CBC and 5µg/ml for PND), the venom exhibited a neuromuscular blocking without any damaging effect on the muscle integrity. At higher concentration (20µg/ml for PND), together with the neuromuscular blockade, there was a moderate myonecrosis. The results show differences between mammalian and avian preparations in response to venom concentration; the avian preparation was more sensitive to venom neurotoxic effect than the mammalian preparation. The possible presynaptic mechanism underlying the neuromuscular blocking effect was reinforced by the observed increase in MEPPs at the same time (at 15min) when the facilitation of twitch tension occurred. These results indicate that the B. marajoensis venom produced neuromuscular blockade, which appeared to be presynaptic at low concentrations with a postsynaptic component at high concentrations, leading to muscle oedema. These observations demand the fractionation of the crude venom and characterization of its active components for a better understanding of its biological dynamics.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual de Campinas, Departamento de Farmacologia, Faculdade de Ciências MédicasUniversidade Estadual de Campinas, Departamento de Histologia e Embriologia, Instituto de BiologiaUniversidade Estadual de Campinas, Departamento de Bioquímica, Instituto de BiologiaUniversidade Estadual Paulista, Departamento de Morfologia, Instituto de Biociências de BotucatuUniversidade Estadual Paulista, Departamento de Farmacologia, Instituto de Biociências de BotucatuUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Cavalcante, Walter Luiz Garrido [UNESP]Hernadez-Oliveira, SaraguaciGalbiatti, CharleneRandazzo-Moura, PriscilaRocha, ThalitaPonce-Souto, LuisMarangoni, SérgioPai-Silva, Maeli Dal [UNESP]Gallacci, Márcia [UNESP]Cruz-Höfling, Maria Alice daRodrigues-Simioni, Léa2016-07-07T12:36:37Z2016-07-07T12:36:37Z2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article37-41application/pdfhttp://libpubmedia.co.uk/biological-characterization-of-bothrops-marajoensis-snake-venom/Journal of Venom Research, v. 2, p. 37-41, 2011.2044-0324http://hdl.handle.net/11449/141050ISSN2044-0324-2011-02-37-41.pdf9353490382598257Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venom Researchinfo:eu-repo/semantics/openAccess2023-12-17T06:19:38Zoai:repositorio.unesp.br:11449/141050Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-17T06:19:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biological characterization of Bothrops marajoensis snake venom |
title |
Biological characterization of Bothrops marajoensis snake venom |
spellingShingle |
Biological characterization of Bothrops marajoensis snake venom Cavalcante, Walter Luiz Garrido [UNESP] Marajó lancehead Neuromuscular junction Neurotoxicity Myotoxicity Presynaptic effects |
title_short |
Biological characterization of Bothrops marajoensis snake venom |
title_full |
Biological characterization of Bothrops marajoensis snake venom |
title_fullStr |
Biological characterization of Bothrops marajoensis snake venom |
title_full_unstemmed |
Biological characterization of Bothrops marajoensis snake venom |
title_sort |
Biological characterization of Bothrops marajoensis snake venom |
author |
Cavalcante, Walter Luiz Garrido [UNESP] |
author_facet |
Cavalcante, Walter Luiz Garrido [UNESP] Hernadez-Oliveira, Saraguaci Galbiatti, Charlene Randazzo-Moura, Priscila Rocha, Thalita Ponce-Souto, Luis Marangoni, Sérgio Pai-Silva, Maeli Dal [UNESP] Gallacci, Márcia [UNESP] Cruz-Höfling, Maria Alice da Rodrigues-Simioni, Léa |
author_role |
author |
author2 |
Hernadez-Oliveira, Saraguaci Galbiatti, Charlene Randazzo-Moura, Priscila Rocha, Thalita Ponce-Souto, Luis Marangoni, Sérgio Pai-Silva, Maeli Dal [UNESP] Gallacci, Márcia [UNESP] Cruz-Höfling, Maria Alice da Rodrigues-Simioni, Léa |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Cavalcante, Walter Luiz Garrido [UNESP] Hernadez-Oliveira, Saraguaci Galbiatti, Charlene Randazzo-Moura, Priscila Rocha, Thalita Ponce-Souto, Luis Marangoni, Sérgio Pai-Silva, Maeli Dal [UNESP] Gallacci, Márcia [UNESP] Cruz-Höfling, Maria Alice da Rodrigues-Simioni, Léa |
dc.subject.por.fl_str_mv |
Marajó lancehead Neuromuscular junction Neurotoxicity Myotoxicity Presynaptic effects |
topic |
Marajó lancehead Neuromuscular junction Neurotoxicity Myotoxicity Presynaptic effects |
description |
This study describes the effects of Bothrops marajoensis venom (Marajó lancehead) on isolated neuromuscular preparations of chick biventer cervicis (CBC) and mouse phrenic nerve-diaphragm (PND). At low concentrations (1µg/ml for CBC and 5µg/ml for PND), the venom exhibited a neuromuscular blocking without any damaging effect on the muscle integrity. At higher concentration (20µg/ml for PND), together with the neuromuscular blockade, there was a moderate myonecrosis. The results show differences between mammalian and avian preparations in response to venom concentration; the avian preparation was more sensitive to venom neurotoxic effect than the mammalian preparation. The possible presynaptic mechanism underlying the neuromuscular blocking effect was reinforced by the observed increase in MEPPs at the same time (at 15min) when the facilitation of twitch tension occurred. These results indicate that the B. marajoensis venom produced neuromuscular blockade, which appeared to be presynaptic at low concentrations with a postsynaptic component at high concentrations, leading to muscle oedema. These observations demand the fractionation of the crude venom and characterization of its active components for a better understanding of its biological dynamics. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2016-07-07T12:36:37Z 2016-07-07T12:36:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://libpubmedia.co.uk/biological-characterization-of-bothrops-marajoensis-snake-venom/ Journal of Venom Research, v. 2, p. 37-41, 2011. 2044-0324 http://hdl.handle.net/11449/141050 ISSN2044-0324-2011-02-37-41.pdf 9353490382598257 |
url |
http://libpubmedia.co.uk/biological-characterization-of-bothrops-marajoensis-snake-venom/ http://hdl.handle.net/11449/141050 |
identifier_str_mv |
Journal of Venom Research, v. 2, p. 37-41, 2011. 2044-0324 ISSN2044-0324-2011-02-37-41.pdf 9353490382598257 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venom Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
37-41 application/pdf |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046988883689472 |