Antibacterial activity of phenyl isothiocyanate on Escherichia coli and Staphylococcus aureus
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/25735 |
Resumo: | The present study has been aimed to assess the antibacterial effects of the glucosinolate hydrolysis product phenyl isothiocyanate (PITC) against Escherichia coli and Staphylococcus aureus. Aspects on the antibacterial mode of action of PITC have also been characterized, such as the changes on surface physicochemical characteristics and membrane damage. The minimum inhibitory concentration of PITC was 1000 µg/mL, for both bacteria. The antimicrobial potential of PITC was compared with selected antibiotics (ciprofloxacin, erythromycin, streptomycin, tetracycline and spectinomycin),that reported a moderate effect. The combination of PITC with ciprofloxacin and erythromycin against S.aureus exhibited a good antimicrobial efficacy, due to an additive effect (the diameter of inhibition zones increased from 30 to 40 mm for ciprofloxacin and almost the double for erythromycin). The other combinations reported unsatisfactory results against both bacteria. The study of the physiological changes induced by PITC action demonstrated the interaction between the electrophilic compound and the bacterial cells at several points that causes changes in membrane properties (decreases negative surface charge, increases surface hydrophilicity and electron donor characteristics). PITC was also found to disturb membrane function, as manifested by phenomena such as cellular disruption and loss of membrane integrity, triggering cell death. |
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Antibacterial activity of phenyl isothiocyanate on Escherichia coli and Staphylococcus aureusAntibiotic-chemical synergyAntimicrobial actionMechanisms of actionPhenyl isothiocyanateScience & TechnologyThe present study has been aimed to assess the antibacterial effects of the glucosinolate hydrolysis product phenyl isothiocyanate (PITC) against Escherichia coli and Staphylococcus aureus. Aspects on the antibacterial mode of action of PITC have also been characterized, such as the changes on surface physicochemical characteristics and membrane damage. The minimum inhibitory concentration of PITC was 1000 µg/mL, for both bacteria. The antimicrobial potential of PITC was compared with selected antibiotics (ciprofloxacin, erythromycin, streptomycin, tetracycline and spectinomycin),that reported a moderate effect. The combination of PITC with ciprofloxacin and erythromycin against S.aureus exhibited a good antimicrobial efficacy, due to an additive effect (the diameter of inhibition zones increased from 30 to 40 mm for ciprofloxacin and almost the double for erythromycin). The other combinations reported unsatisfactory results against both bacteria. The study of the physiological changes induced by PITC action demonstrated the interaction between the electrophilic compound and the bacterial cells at several points that causes changes in membrane properties (decreases negative surface charge, increases surface hydrophilicity and electron donor characteristics). PITC was also found to disturb membrane function, as manifested by phenomena such as cellular disruption and loss of membrane integrity, triggering cell death.This work was supported by Operational Programme for Competitiveness Factors - COMPETE and by FCT - Portuguese Foundation for Science and Technology through Projects Bioresist - PTDC/EBB-EBI/105085/2008; Phytodisinfectants - PTDC/DTP-SAP/1078/2012; the PhD grant (SFRH/BD/63398/2009-Anabela Borges) and the Post-Doctoral grant (SFRH/BPD/81982/2011-Lucia C. Simoes).Bentham Science PublishersBentham Science PublishersUniversidade do MinhoAbreu, Ana C.Borges, AnabelaSimões, Lúcia C.Saavedra, Maria J.Simões, M.20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/25735eng1573-406410.2174/157340641130905001622974327info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:56:50Zoai:repositorium.sdum.uminho.pt:1822/25735Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:46:30.427279Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antibacterial activity of phenyl isothiocyanate on Escherichia coli and Staphylococcus aureus |
title |
Antibacterial activity of phenyl isothiocyanate on Escherichia coli and Staphylococcus aureus |
spellingShingle |
Antibacterial activity of phenyl isothiocyanate on Escherichia coli and Staphylococcus aureus Abreu, Ana C. Antibiotic-chemical synergy Antimicrobial action Mechanisms of action Phenyl isothiocyanate Science & Technology |
title_short |
Antibacterial activity of phenyl isothiocyanate on Escherichia coli and Staphylococcus aureus |
title_full |
Antibacterial activity of phenyl isothiocyanate on Escherichia coli and Staphylococcus aureus |
title_fullStr |
Antibacterial activity of phenyl isothiocyanate on Escherichia coli and Staphylococcus aureus |
title_full_unstemmed |
Antibacterial activity of phenyl isothiocyanate on Escherichia coli and Staphylococcus aureus |
title_sort |
Antibacterial activity of phenyl isothiocyanate on Escherichia coli and Staphylococcus aureus |
author |
Abreu, Ana C. |
author_facet |
Abreu, Ana C. Borges, Anabela Simões, Lúcia C. Saavedra, Maria J. Simões, M. |
author_role |
author |
author2 |
Borges, Anabela Simões, Lúcia C. Saavedra, Maria J. Simões, M. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Abreu, Ana C. Borges, Anabela Simões, Lúcia C. Saavedra, Maria J. Simões, M. |
dc.subject.por.fl_str_mv |
Antibiotic-chemical synergy Antimicrobial action Mechanisms of action Phenyl isothiocyanate Science & Technology |
topic |
Antibiotic-chemical synergy Antimicrobial action Mechanisms of action Phenyl isothiocyanate Science & Technology |
description |
The present study has been aimed to assess the antibacterial effects of the glucosinolate hydrolysis product phenyl isothiocyanate (PITC) against Escherichia coli and Staphylococcus aureus. Aspects on the antibacterial mode of action of PITC have also been characterized, such as the changes on surface physicochemical characteristics and membrane damage. The minimum inhibitory concentration of PITC was 1000 µg/mL, for both bacteria. The antimicrobial potential of PITC was compared with selected antibiotics (ciprofloxacin, erythromycin, streptomycin, tetracycline and spectinomycin),that reported a moderate effect. The combination of PITC with ciprofloxacin and erythromycin against S.aureus exhibited a good antimicrobial efficacy, due to an additive effect (the diameter of inhibition zones increased from 30 to 40 mm for ciprofloxacin and almost the double for erythromycin). The other combinations reported unsatisfactory results against both bacteria. The study of the physiological changes induced by PITC action demonstrated the interaction between the electrophilic compound and the bacterial cells at several points that causes changes in membrane properties (decreases negative surface charge, increases surface hydrophilicity and electron donor characteristics). PITC was also found to disturb membrane function, as manifested by phenomena such as cellular disruption and loss of membrane integrity, triggering cell death. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2013-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/25735 |
url |
http://hdl.handle.net/1822/25735 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1573-4064 10.2174/1573406411309050016 22974327 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Bentham Science Publishers Bentham Science Publishers |
publisher.none.fl_str_mv |
Bentham Science Publishers Bentham Science Publishers |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799132220967878656 |