Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content

Detalhes bibliográficos
Autor(a) principal: Carvalho, Patrícia M.
Data de Publicação: 2022
Outros Autores: Marguí, Eva, Kubala-Kukuś, Aldona, Banaś, Dariusz, Machado, Jorge, Casal, Diogo, Pais, Diogo, Santos, José Paulo, Pessanha, Sofia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/145891
Resumo: Publisher Copyright: © 2022 The Authors
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spelling Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose contentEDXRFHuman tissuesMultielemental analysisSample preparationTXRFAnalytical ChemistryAtomic and Molecular Physics, and OpticsInstrumentationSpectroscopyPublisher Copyright: © 2022 The AuthorsElemental content plays an important role in biological processes, and so, the multielemental analysis of human tissue samples is required in biomedical research. Still, the small amount of available biological samples and the adipose content of the samples can be major setbacks for the accurate determination of elemental content. In this study, we explored the potential of several analytical approaches combined with total reflection X-ray fluorescence spectrometry (TXRF) for multielemental analysis of human tissues with different adipose content (colon, heart, liver, lung, muscle, intestine, skin, stomach, uterus, bladder and aorta). The capabilities and limitations of different sample treatment procedures (suspension and acidic digestion) and two TXRF systems with different anode configurations (Mo and W X-ray tubes) have been evaluated for such purpose. Results showed that for tissues with a higher fat content (e.g., skin, and intestine) the best strategy was the acidic digestion of the sample before TXRF analysis. However, for other tissues, acceptable results were obtained by suspending 20 mg of powdered material in 1 mL of 2 M nitric acid. A further enhancement of the limits of detection and accuracy of the results was achieved if using Mo-TXRF systems, especially for the determination of low Z elements (e.g., K, and Ca) and of elements present at low concentrations (e.g., Cu) in the human tissues. Finally, results by TXRF analysis were compared with those obtained with μ-EDXRF and ICP-OES, and a good agreement was obtained.DF – Departamento de FísicaNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNCarvalho, Patrícia M.Marguí, EvaKubala-Kukuś, AldonaBanaś, DariuszMachado, JorgeCasal, DiogoPais, DiogoSantos, José PauloPessanha, Sofia2022-11-29T22:12:15Z2022-122022-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/145891eng0584-8547PURE: 47732064https://doi.org/10.1016/j.sab.2022.106548info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:26:36Zoai:run.unl.pt:10362/145891Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:19.585819Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content
title Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content
spellingShingle Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content
Carvalho, Patrícia M.
EDXRF
Human tissues
Multielemental analysis
Sample preparation
TXRF
Analytical Chemistry
Atomic and Molecular Physics, and Optics
Instrumentation
Spectroscopy
title_short Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content
title_full Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content
title_fullStr Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content
title_full_unstemmed Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content
title_sort Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content
author Carvalho, Patrícia M.
author_facet Carvalho, Patrícia M.
Marguí, Eva
Kubala-Kukuś, Aldona
Banaś, Dariusz
Machado, Jorge
Casal, Diogo
Pais, Diogo
Santos, José Paulo
Pessanha, Sofia
author_role author
author2 Marguí, Eva
Kubala-Kukuś, Aldona
Banaś, Dariusz
Machado, Jorge
Casal, Diogo
Pais, Diogo
Santos, José Paulo
Pessanha, Sofia
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DF – Departamento de Física
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Carvalho, Patrícia M.
Marguí, Eva
Kubala-Kukuś, Aldona
Banaś, Dariusz
Machado, Jorge
Casal, Diogo
Pais, Diogo
Santos, José Paulo
Pessanha, Sofia
dc.subject.por.fl_str_mv EDXRF
Human tissues
Multielemental analysis
Sample preparation
TXRF
Analytical Chemistry
Atomic and Molecular Physics, and Optics
Instrumentation
Spectroscopy
topic EDXRF
Human tissues
Multielemental analysis
Sample preparation
TXRF
Analytical Chemistry
Atomic and Molecular Physics, and Optics
Instrumentation
Spectroscopy
description Publisher Copyright: © 2022 The Authors
publishDate 2022
dc.date.none.fl_str_mv 2022-11-29T22:12:15Z
2022-12
2022-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/145891
url http://hdl.handle.net/10362/145891
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0584-8547
PURE: 47732064
https://doi.org/10.1016/j.sab.2022.106548
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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