Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/145891 |
Resumo: | Publisher Copyright: © 2022 The Authors |
id |
RCAP_a0120f33f262222e18b6123b7d65a361 |
---|---|
oai_identifier_str |
oai:run.unl.pt:10362/145891 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose contentEDXRFHuman tissuesMultielemental analysisSample preparationTXRFAnalytical ChemistryAtomic and Molecular Physics, and OpticsInstrumentationSpectroscopyPublisher Copyright: © 2022 The AuthorsElemental content plays an important role in biological processes, and so, the multielemental analysis of human tissue samples is required in biomedical research. Still, the small amount of available biological samples and the adipose content of the samples can be major setbacks for the accurate determination of elemental content. In this study, we explored the potential of several analytical approaches combined with total reflection X-ray fluorescence spectrometry (TXRF) for multielemental analysis of human tissues with different adipose content (colon, heart, liver, lung, muscle, intestine, skin, stomach, uterus, bladder and aorta). The capabilities and limitations of different sample treatment procedures (suspension and acidic digestion) and two TXRF systems with different anode configurations (Mo and W X-ray tubes) have been evaluated for such purpose. Results showed that for tissues with a higher fat content (e.g., skin, and intestine) the best strategy was the acidic digestion of the sample before TXRF analysis. However, for other tissues, acceptable results were obtained by suspending 20 mg of powdered material in 1 mL of 2 M nitric acid. A further enhancement of the limits of detection and accuracy of the results was achieved if using Mo-TXRF systems, especially for the determination of low Z elements (e.g., K, and Ca) and of elements present at low concentrations (e.g., Cu) in the human tissues. Finally, results by TXRF analysis were compared with those obtained with μ-EDXRF and ICP-OES, and a good agreement was obtained.DF – Departamento de FísicaNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNCarvalho, Patrícia M.Marguí, EvaKubala-Kukuś, AldonaBanaś, DariuszMachado, JorgeCasal, DiogoPais, DiogoSantos, José PauloPessanha, Sofia2022-11-29T22:12:15Z2022-122022-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/145891eng0584-8547PURE: 47732064https://doi.org/10.1016/j.sab.2022.106548info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:26:36Zoai:run.unl.pt:10362/145891Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:19.585819Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content |
title |
Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content |
spellingShingle |
Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content Carvalho, Patrícia M. EDXRF Human tissues Multielemental analysis Sample preparation TXRF Analytical Chemistry Atomic and Molecular Physics, and Optics Instrumentation Spectroscopy |
title_short |
Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content |
title_full |
Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content |
title_fullStr |
Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content |
title_full_unstemmed |
Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content |
title_sort |
Evaluation of different analytical approaches using total reflection X-ray fluorescence systems for multielemental analysis of human tissues with different adipose content |
author |
Carvalho, Patrícia M. |
author_facet |
Carvalho, Patrícia M. Marguí, Eva Kubala-Kukuś, Aldona Banaś, Dariusz Machado, Jorge Casal, Diogo Pais, Diogo Santos, José Paulo Pessanha, Sofia |
author_role |
author |
author2 |
Marguí, Eva Kubala-Kukuś, Aldona Banaś, Dariusz Machado, Jorge Casal, Diogo Pais, Diogo Santos, José Paulo Pessanha, Sofia |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
DF – Departamento de Física NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) RUN |
dc.contributor.author.fl_str_mv |
Carvalho, Patrícia M. Marguí, Eva Kubala-Kukuś, Aldona Banaś, Dariusz Machado, Jorge Casal, Diogo Pais, Diogo Santos, José Paulo Pessanha, Sofia |
dc.subject.por.fl_str_mv |
EDXRF Human tissues Multielemental analysis Sample preparation TXRF Analytical Chemistry Atomic and Molecular Physics, and Optics Instrumentation Spectroscopy |
topic |
EDXRF Human tissues Multielemental analysis Sample preparation TXRF Analytical Chemistry Atomic and Molecular Physics, and Optics Instrumentation Spectroscopy |
description |
Publisher Copyright: © 2022 The Authors |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-29T22:12:15Z 2022-12 2022-12-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/145891 |
url |
http://hdl.handle.net/10362/145891 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0584-8547 PURE: 47732064 https://doi.org/10.1016/j.sab.2022.106548 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799138115060760576 |