Development of tools for genetic manipulation of Staphylococcus aureus

Detalhes bibliográficos
Autor(a) principal: Serra, Lúcia Branco
Data de Publicação: 2017
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/85392
Resumo: "With the global distribution of multidrug resistant Staphyloccocus aureus, a human pathogen responsible for thousands of deaths every year, there is an increasing need to study its mechanisms of resistance as well as discover new antibiotics that effectively interfere with its growth and division. However, despite the growing interest in studying this bacteria, there are only a few tools currently available for the genetic manipulation of S. aureus. In this study we developed genetic tools that could facilitate DNA insertion and gene repression in S. aureus: pKILL plasmid and CRISPRi.(...)"
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spelling Development of tools for genetic manipulation of Staphylococcus aureusStaphylococcus aureusfluorescence microscopygenetic manipulationCRISPRiMedical Microbiology"With the global distribution of multidrug resistant Staphyloccocus aureus, a human pathogen responsible for thousands of deaths every year, there is an increasing need to study its mechanisms of resistance as well as discover new antibiotics that effectively interfere with its growth and division. However, despite the growing interest in studying this bacteria, there are only a few tools currently available for the genetic manipulation of S. aureus. In this study we developed genetic tools that could facilitate DNA insertion and gene repression in S. aureus: pKILL plasmid and CRISPRi.(...)"Pinho, MarianaRUNSerra, Lúcia Branco2020-12-31T01:30:41Z20172017-122017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/85392enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:38:23Zoai:run.unl.pt:10362/85392Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:36:37.146572Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Development of tools for genetic manipulation of Staphylococcus aureus
title Development of tools for genetic manipulation of Staphylococcus aureus
spellingShingle Development of tools for genetic manipulation of Staphylococcus aureus
Serra, Lúcia Branco
Staphylococcus aureus
fluorescence microscopy
genetic manipulation
CRISPRi
Medical Microbiology
title_short Development of tools for genetic manipulation of Staphylococcus aureus
title_full Development of tools for genetic manipulation of Staphylococcus aureus
title_fullStr Development of tools for genetic manipulation of Staphylococcus aureus
title_full_unstemmed Development of tools for genetic manipulation of Staphylococcus aureus
title_sort Development of tools for genetic manipulation of Staphylococcus aureus
author Serra, Lúcia Branco
author_facet Serra, Lúcia Branco
author_role author
dc.contributor.none.fl_str_mv Pinho, Mariana
RUN
dc.contributor.author.fl_str_mv Serra, Lúcia Branco
dc.subject.por.fl_str_mv Staphylococcus aureus
fluorescence microscopy
genetic manipulation
CRISPRi
Medical Microbiology
topic Staphylococcus aureus
fluorescence microscopy
genetic manipulation
CRISPRi
Medical Microbiology
description "With the global distribution of multidrug resistant Staphyloccocus aureus, a human pathogen responsible for thousands of deaths every year, there is an increasing need to study its mechanisms of resistance as well as discover new antibiotics that effectively interfere with its growth and division. However, despite the growing interest in studying this bacteria, there are only a few tools currently available for the genetic manipulation of S. aureus. In this study we developed genetic tools that could facilitate DNA insertion and gene repression in S. aureus: pKILL plasmid and CRISPRi.(...)"
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-12
2017-01-01T00:00:00Z
2020-12-31T01:30:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/85392
url http://hdl.handle.net/10362/85392
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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