Magnetic Precipitation: A New Platform for Protein Purification
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/141851 |
Resumo: | PD/BD/105753/2014 UIDB/ 04378/2020 POCI-01-0145-FEDER-007728 PTDC/BII-BIO/28878/2017 |
| id |
RCAP_a0b63f03af8a011e913f7bbb89d19077 |
|---|---|
| oai_identifier_str |
oai:run.unl.pt:10362/141851 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
Magnetic Precipitation: A New Platform for Protein Purificationaffinity magnetic precipitationantibody purificationdownstreammagnetic nanoparticlesmethod developmentApplied Microbiology and BiotechnologyMolecular MedicinePD/BD/105753/2014 UIDB/ 04378/2020 POCI-01-0145-FEDER-007728 PTDC/BII-BIO/28878/2017One of the trends in downstream processing comprises the use of “anything-but-chromatography” methods to overcome the current downfalls of standard packed-bed chromatography. Precipitation and magnetic separation are two techniques already proven to accomplish protein purification from complex media, yet never used in synergy. With the aim to capture antibodies directly from crude extracts, a new approach combining precipitation and magnetic separation is developed and named as affinity magnetic precipitation. A precipitation screening, based on the Hofmeister series, and a commercial precipitation kit are tested with affinity magnetic particles to assess the best condition for antibody capture from human serum plasma and clarified cell supernatant. The best conditions are obtained when using PEG3350 as precipitant at 4 °C for 1 h, reaching 80% purity and 50% recovery of polyclonal antibodies from plasma, and 99% purity with 97% recovery yield of anti-TNFα mAb from cell supernatants. These results show that the synergetic use of precipitation and magnetic separation can represent an alternative for the efficient capture of antibodies.DQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitRUNDos Santos, RaquelIria, InêsManuel, Ana M.Leandro, Ana P.Madeira, Catarina A. C.Goncalves, JoãoCarvalho, Ana LuísaRoque, Ana Cecília2022-07-13T22:19:57Z2020-09-012020-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/141851eng1860-6768PURE: 18808618https://doi.org/10.1002/biot.202000151info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:19:10Zoai:run.unl.pt:10362/141851Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:50:04.415130Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Magnetic Precipitation: A New Platform for Protein Purification |
| title |
Magnetic Precipitation: A New Platform for Protein Purification |
| spellingShingle |
Magnetic Precipitation: A New Platform for Protein Purification Dos Santos, Raquel affinity magnetic precipitation antibody purification downstream magnetic nanoparticles method development Applied Microbiology and Biotechnology Molecular Medicine |
| title_short |
Magnetic Precipitation: A New Platform for Protein Purification |
| title_full |
Magnetic Precipitation: A New Platform for Protein Purification |
| title_fullStr |
Magnetic Precipitation: A New Platform for Protein Purification |
| title_full_unstemmed |
Magnetic Precipitation: A New Platform for Protein Purification |
| title_sort |
Magnetic Precipitation: A New Platform for Protein Purification |
| author |
Dos Santos, Raquel |
| author_facet |
Dos Santos, Raquel Iria, Inês Manuel, Ana M. Leandro, Ana P. Madeira, Catarina A. C. Goncalves, João Carvalho, Ana Luísa Roque, Ana Cecília |
| author_role |
author |
| author2 |
Iria, Inês Manuel, Ana M. Leandro, Ana P. Madeira, Catarina A. C. Goncalves, João Carvalho, Ana Luísa Roque, Ana Cecília |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
DQ - Departamento de Química UCIBIO - Applied Molecular Biosciences Unit RUN |
| dc.contributor.author.fl_str_mv |
Dos Santos, Raquel Iria, Inês Manuel, Ana M. Leandro, Ana P. Madeira, Catarina A. C. Goncalves, João Carvalho, Ana Luísa Roque, Ana Cecília |
| dc.subject.por.fl_str_mv |
affinity magnetic precipitation antibody purification downstream magnetic nanoparticles method development Applied Microbiology and Biotechnology Molecular Medicine |
| topic |
affinity magnetic precipitation antibody purification downstream magnetic nanoparticles method development Applied Microbiology and Biotechnology Molecular Medicine |
| description |
PD/BD/105753/2014 UIDB/ 04378/2020 POCI-01-0145-FEDER-007728 PTDC/BII-BIO/28878/2017 |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-09-01 2020-09-01T00:00:00Z 2022-07-13T22:19:57Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/141851 |
| url |
http://hdl.handle.net/10362/141851 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1860-6768 PURE: 18808618 https://doi.org/10.1002/biot.202000151 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799138098040274944 |