Magnetic nanoparticles for biocatalysis and bioseparation

Detalhes bibliográficos
Autor(a) principal: Santana, Sara Daniela Filipe
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/6259
Resumo: Dissertação para obtenção do Grau de Mestre em Biotecnologia
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spelling Magnetic nanoparticles for biocatalysis and bioseparationMagnetic nanoparticlesBiopolymersEnzyme cleavageAffinity ligandsPurificationDissertação para obtenção do Grau de Mestre em BiotecnologiaThis work aimed to prepare iron oxide magnetic nanoparticles (MNPs) coated with biopolymers and further modified with enzymes and synthetic affinity ligands in order to study their applications in biocatalysis and bioseparation areas. The adsorption of different biopolymers - Gum Arabic (Ga), Dextran (Dex) and Extracellular Polysaccharide (EPS) - was performed during the synthesis of the particles, and the amount of polymer bound was determined and compared to the adsorption profiles of each polymer. Additionally, the storage and chemical modification stability of these supports was also evaluated. All supports showed high stability. The eventual application of these magnetic supports in biocatalysis was evaluated through the immobilization of an enzyme, Enterokinase (EK), through two distinct chemistries. The enzyme was then tested with a synthetic substrate and two fusion proteins. After the enzyme’s immobilization, its activity was observed to decrease, which was compensated by its re-utilization up to ten times. The most promising magnetic support was the MNP_Dex since it presented an activity retention of 35% and a conversion percentage of 0.4% in the first reaction cycle. The utilization of magnetic supports for the purification of IgG was tested through the immobilization of the synthetic affinity ligand 22/8 using three different methods. The MNP_Dex support modified with the ligand synthesized directly on the solid support adsorbed around 130 mg of IgG/g of MNP and presented less nonspecific adsorption. Moreover, this support presented specificity and capability to adsorb IgG and its fragments from crude extracts expressed in yeast and mammalian cells.Faculdade de Ciências e TecnologiaRoque, AnaRUNSantana, Sara Daniela Filipe2011-12-02T10:51:23Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/6259enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:37:26Zoai:run.unl.pt:10362/6259Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:16:48.317738Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Magnetic nanoparticles for biocatalysis and bioseparation
title Magnetic nanoparticles for biocatalysis and bioseparation
spellingShingle Magnetic nanoparticles for biocatalysis and bioseparation
Santana, Sara Daniela Filipe
Magnetic nanoparticles
Biopolymers
Enzyme cleavage
Affinity ligands
Purification
title_short Magnetic nanoparticles for biocatalysis and bioseparation
title_full Magnetic nanoparticles for biocatalysis and bioseparation
title_fullStr Magnetic nanoparticles for biocatalysis and bioseparation
title_full_unstemmed Magnetic nanoparticles for biocatalysis and bioseparation
title_sort Magnetic nanoparticles for biocatalysis and bioseparation
author Santana, Sara Daniela Filipe
author_facet Santana, Sara Daniela Filipe
author_role author
dc.contributor.none.fl_str_mv Roque, Ana
RUN
dc.contributor.author.fl_str_mv Santana, Sara Daniela Filipe
dc.subject.por.fl_str_mv Magnetic nanoparticles
Biopolymers
Enzyme cleavage
Affinity ligands
Purification
topic Magnetic nanoparticles
Biopolymers
Enzyme cleavage
Affinity ligands
Purification
description Dissertação para obtenção do Grau de Mestre em Biotecnologia
publishDate 2011
dc.date.none.fl_str_mv 2011-12-02T10:51:23Z
2011
2011-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/6259
url http://hdl.handle.net/10362/6259
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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