Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation

Detalhes bibliográficos
Autor(a) principal: Craveiro, R.
Data de Publicação: 2021
Outros Autores: Castro, V. I. B., Viciosa, M. T., Dionisio, M., Reis, R. L., Duarte, A. R. C., Paiva, A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/73487
Resumo: Natural deep eutectic systems (NADES), which have applications as solvents for both engineering and life sci-ences, are mainly composed of sugars, aminoacids or organic acids. In this work NADES composed by glucose,urea and proline (G:U:P in a molar ratio of 1:1:1) and proline and glucose (P:G 5:3) were prepared and addedin different mass fractions to water.By differential scanning calorimetry it was verified as the crystallization tendency of water is modified even forlow fraction of NADES added. This is also observed by polarized optical microscopy which allowed followingthe formation of crystals with different crystalline morphologies as bulk water. Calorimetric data also shown asthe crystallization temperature decreases for all P:G mixtures and this shift is more accentuated for weight frac-tion of NADES higher than 0.5. Crystallization is totally suppressed for NADES fraction higher than 0.7.NADES/water mixtures cytotoxicity was evaluated in vitro, revealing that they are less toxic as compared withthe commonly used cryoprotective additives as dimethyl sulfoxide (DMSO). Additionally, cell viability testswith cell lines cryopreserved using DMSO and both the prepared NADES showed comparable viability.This work combines thermophysical data on NADES and evaluates itâ s in vitro performance, providing cues fortheir use in cryopreservation applications.
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spelling Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservationCryopreservationNatural Deep-eutectic solventsWater mixturesNADESGlucoseUreaProlineCytotoxicityThermal propertiesEngenharia e Tecnologia::Biotecnologia AmbientalScience & TechnologyNatural deep eutectic systems (NADES), which have applications as solvents for both engineering and life sci-ences, are mainly composed of sugars, aminoacids or organic acids. In this work NADES composed by glucose,urea and proline (G:U:P in a molar ratio of 1:1:1) and proline and glucose (P:G 5:3) were prepared and addedin different mass fractions to water.By differential scanning calorimetry it was verified as the crystallization tendency of water is modified even forlow fraction of NADES added. This is also observed by polarized optical microscopy which allowed followingthe formation of crystals with different crystalline morphologies as bulk water. Calorimetric data also shown asthe crystallization temperature decreases for all P:G mixtures and this shift is more accentuated for weight frac-tion of NADES higher than 0.5. Crystallization is totally suppressed for NADES fraction higher than 0.7.NADES/water mixtures cytotoxicity was evaluated in vitro, revealing that they are less toxic as compared withthe commonly used cryoprotective additives as dimethyl sulfoxide (DMSO). Additionally, cell viability testswith cell lines cryopreserved using DMSO and both the prepared NADES showed comparable viability.This work combines thermophysical data on NADES and evaluates itâ s in vitro performance, providing cues fortheir use in cryopreservation applications.European Union Horizon 2020 Program under the agreement number ERC-2016-CoG 725034 (ERC Consolidator Grant Des.solve). This research was also funded by PTDC/EQU-EQU/29851/2017. A. Paiva acknowledges the financial support from project IF/01146/2015 attributed within the 2015 FCT researcher program. This work was supported by the Associate Laboratory for Green Chemistry - LAQV which is financed by national funds from FCT/MCTES (UIDB/50006/2020 and UIDP/50006/2020)ElsevierUniversidade do MinhoCraveiro, R.Castro, V. I. B.Viciosa, M. T.Dionisio, M.Reis, R. L.Duarte, A. R. C.Paiva, A.2021-052021-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/73487engCraveiro R., Castro V. I. B., Viciosa M. T., Dionisio M., Reis R. L., Duarte A. R. C., Paiva A. Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation, Journal of Molecular Liquids, Vol. 329, pp. 115533, doi:10.1016/j.molliq.2021.115533, 20210167-732210.1016/j.molliq.2021.115533https://www.sciencedirect.com/science/article/pii/S0167732221002592info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:53:56Zoai:repositorium.sdum.uminho.pt:1822/73487Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:53:25.567472Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation
title Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation
spellingShingle Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation
Craveiro, R.
Cryopreservation
Natural Deep-eutectic solvents
Water mixtures
NADES
Glucose
Urea
Proline
Cytotoxicity
Thermal properties
Engenharia e Tecnologia::Biotecnologia Ambiental
Science & Technology
title_short Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation
title_full Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation
title_fullStr Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation
title_full_unstemmed Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation
title_sort Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation
author Craveiro, R.
author_facet Craveiro, R.
Castro, V. I. B.
Viciosa, M. T.
Dionisio, M.
Reis, R. L.
Duarte, A. R. C.
Paiva, A.
author_role author
author2 Castro, V. I. B.
Viciosa, M. T.
Dionisio, M.
Reis, R. L.
Duarte, A. R. C.
Paiva, A.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Craveiro, R.
Castro, V. I. B.
Viciosa, M. T.
Dionisio, M.
Reis, R. L.
Duarte, A. R. C.
Paiva, A.
dc.subject.por.fl_str_mv Cryopreservation
Natural Deep-eutectic solvents
Water mixtures
NADES
Glucose
Urea
Proline
Cytotoxicity
Thermal properties
Engenharia e Tecnologia::Biotecnologia Ambiental
Science & Technology
topic Cryopreservation
Natural Deep-eutectic solvents
Water mixtures
NADES
Glucose
Urea
Proline
Cytotoxicity
Thermal properties
Engenharia e Tecnologia::Biotecnologia Ambiental
Science & Technology
description Natural deep eutectic systems (NADES), which have applications as solvents for both engineering and life sci-ences, are mainly composed of sugars, aminoacids or organic acids. In this work NADES composed by glucose,urea and proline (G:U:P in a molar ratio of 1:1:1) and proline and glucose (P:G 5:3) were prepared and addedin different mass fractions to water.By differential scanning calorimetry it was verified as the crystallization tendency of water is modified even forlow fraction of NADES added. This is also observed by polarized optical microscopy which allowed followingthe formation of crystals with different crystalline morphologies as bulk water. Calorimetric data also shown asthe crystallization temperature decreases for all P:G mixtures and this shift is more accentuated for weight frac-tion of NADES higher than 0.5. Crystallization is totally suppressed for NADES fraction higher than 0.7.NADES/water mixtures cytotoxicity was evaluated in vitro, revealing that they are less toxic as compared withthe commonly used cryoprotective additives as dimethyl sulfoxide (DMSO). Additionally, cell viability testswith cell lines cryopreserved using DMSO and both the prepared NADES showed comparable viability.This work combines thermophysical data on NADES and evaluates itâ s in vitro performance, providing cues fortheir use in cryopreservation applications.
publishDate 2021
dc.date.none.fl_str_mv 2021-05
2021-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/73487
url http://hdl.handle.net/1822/73487
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Craveiro R., Castro V. I. B., Viciosa M. T., Dionisio M., Reis R. L., Duarte A. R. C., Paiva A. Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation, Journal of Molecular Liquids, Vol. 329, pp. 115533, doi:10.1016/j.molliq.2021.115533, 2021
0167-7322
10.1016/j.molliq.2021.115533
https://www.sciencedirect.com/science/article/pii/S0167732221002592
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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