Neuroprotective effects of seaweeds against 6-hydroxidopamine-induced cell death on an in vitro human neuroblastoma model

Detalhes bibliográficos
Autor(a) principal: Silva, Joana
Data de Publicação: 2018
Outros Autores: Alves, Celso, Pinteus, Susete, Mendes, Susana, Pedrosa, Rui
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/4991
Resumo: This study had the support of the Fundação para a Ciência e a Tecnologia (FCT) Strategic Project UID/MAR/04292/2013 granted to MARE. Joana Silva, Celso Alves and Susete Pinteus were financial supported by a from FCTFundação para a Ciência e Tecnologia grants SFRH/BD/103255/2014, SFRH/ BD/977641/2013 and SFRH/BD/96203/2013, respectively.
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spelling Neuroprotective effects of seaweeds against 6-hydroxidopamine-induced cell death on an in vitro human neuroblastoma modelParkinson’s diseaseSubstantia nigraMitochondrial membrane potentialCaspase – 3 activityOxidative stressApoptosisMarine natural bioactive compoundsThis study had the support of the Fundação para a Ciência e a Tecnologia (FCT) Strategic Project UID/MAR/04292/2013 granted to MARE. Joana Silva, Celso Alves and Susete Pinteus were financial supported by a from FCTFundação para a Ciência e Tecnologia grants SFRH/BD/103255/2014, SFRH/ BD/977641/2013 and SFRH/BD/96203/2013, respectively.Background: Parkinson’s disease (PD) is a progressive neurodegenerative disorder of the central nervous system. Although the causes of PD pathogenesis remain incomplete, some evidences has suggested that oxidative stress is an important mediator in its pathogenesis. The aim of this study was to evaluate the protective effects of seaweeds with high antioxidant activity on 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in the human neuroblastoma cell line SH-SY5Y, as well as the associated intracellular signaling pathways. Methods: Cell viability studies were assessed by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium (MTT) bromide assay and the intracellular signaling pathways analyzed were: hydrogen peroxide (H2O2) production, changes in the mitochondrial membrane potential and Caspase-3 activity. Results: Exposure of SH-SY5Y cells to 6-OHDA (10–1000 μM) reduced cell’s viability in a concentration and timedependent manner. The data suggest that the cell death induced by 6-OHDA was mediated by an increase of H2O2 production, the depolarization of mitochondrial membrane potential and the increase of Caspase-3 activity. Extracts from S. polyshides, P. pavonica, S. muticum, C. tomentosum and U. compressa revealed to efficiently protect cell’s viability in the presence of 6-OHDA (100 μM; 24 h). These effects appear to be associated with the reduction of H2O2 cell’s production, the protection of mitochondrial membrane’s potential and the reduction of Caspase-3 activity. Conclusions: These results suggest that seaweeds can be a promising source of new compounds with neuroprotective potential.BMCIC-OnlineSilva, JoanaAlves, CelsoPinteus, SuseteMendes, SusanaPedrosa, Rui2020-07-06T14:27:03Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/4991engSilva, J., Alves, C., Pinteus, S., Mendes, S., & Pedrosa, R. (2018). Neuroprotective effects of seaweeds against 6-hydroxidopamine-induced cell death on an in vitro human neuroblastoma model. BMC complementary and alternative medicine, 18(1), 58. https://doi.org/10.1186/s12906-018-2103-21472-688210.1186/s12906-018-2103-2info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-26T18:18:12Zoai:iconline.ipleiria.pt:10400.8/4991Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-26T18:18:12Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Neuroprotective effects of seaweeds against 6-hydroxidopamine-induced cell death on an in vitro human neuroblastoma model
title Neuroprotective effects of seaweeds against 6-hydroxidopamine-induced cell death on an in vitro human neuroblastoma model
spellingShingle Neuroprotective effects of seaweeds against 6-hydroxidopamine-induced cell death on an in vitro human neuroblastoma model
Silva, Joana
Parkinson’s disease
Substantia nigra
Mitochondrial membrane potential
Caspase – 3 activity
Oxidative stress
Apoptosis
Marine natural bioactive compounds
title_short Neuroprotective effects of seaweeds against 6-hydroxidopamine-induced cell death on an in vitro human neuroblastoma model
title_full Neuroprotective effects of seaweeds against 6-hydroxidopamine-induced cell death on an in vitro human neuroblastoma model
title_fullStr Neuroprotective effects of seaweeds against 6-hydroxidopamine-induced cell death on an in vitro human neuroblastoma model
title_full_unstemmed Neuroprotective effects of seaweeds against 6-hydroxidopamine-induced cell death on an in vitro human neuroblastoma model
title_sort Neuroprotective effects of seaweeds against 6-hydroxidopamine-induced cell death on an in vitro human neuroblastoma model
author Silva, Joana
author_facet Silva, Joana
Alves, Celso
Pinteus, Susete
Mendes, Susana
Pedrosa, Rui
author_role author
author2 Alves, Celso
Pinteus, Susete
Mendes, Susana
Pedrosa, Rui
author2_role author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Silva, Joana
Alves, Celso
Pinteus, Susete
Mendes, Susana
Pedrosa, Rui
dc.subject.por.fl_str_mv Parkinson’s disease
Substantia nigra
Mitochondrial membrane potential
Caspase – 3 activity
Oxidative stress
Apoptosis
Marine natural bioactive compounds
topic Parkinson’s disease
Substantia nigra
Mitochondrial membrane potential
Caspase – 3 activity
Oxidative stress
Apoptosis
Marine natural bioactive compounds
description This study had the support of the Fundação para a Ciência e a Tecnologia (FCT) Strategic Project UID/MAR/04292/2013 granted to MARE. Joana Silva, Celso Alves and Susete Pinteus were financial supported by a from FCTFundação para a Ciência e Tecnologia grants SFRH/BD/103255/2014, SFRH/ BD/977641/2013 and SFRH/BD/96203/2013, respectively.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
2020-07-06T14:27:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/4991
url http://hdl.handle.net/10400.8/4991
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva, J., Alves, C., Pinteus, S., Mendes, S., & Pedrosa, R. (2018). Neuroprotective effects of seaweeds against 6-hydroxidopamine-induced cell death on an in vitro human neuroblastoma model. BMC complementary and alternative medicine, 18(1), 58. https://doi.org/10.1186/s12906-018-2103-2
1472-6882
10.1186/s12906-018-2103-2
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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