Seaweeds’ neuroprotective potential set in vitro on a human cellular stress model

Detalhes bibliográficos
Autor(a) principal: Silva, Joana
Data de Publicação: 2020
Outros Autores: Alves, Celso, Pinteus, Susete, Mendes, Susana, Pedrosa, Rui
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/5117
Resumo: Funding This work was supported by the Portuguese Foundation for Science and Technology (FCT) through strategic project UID/MAR/04292/2020 granted to MARE—Marine and Environmental Sciences Centre, through Red2Discovery project (PTDC/MAR-BIO/6149/2014), co-fnanced by COMPETE (POCI-01-0145-FEDER-016791), through Oncologia de Precisão: Terapias e Tecnologias Inovadoras project (POINT4PAC) (SAICTPAC/0019/2015-LISBOA-01-0145-FEDER-016405) and through CrossAtlantic project (PTDC/BIA-OUT/29250/2017), co-fnanced by COMPETE (POCI-01-0145-FEDER-029250). This work was also funded by the Integrated Programme of SR&TD Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate (reference Centro-01-0145-FEDER-000018), co-funded by Centro 2020 Programme, Portugal 2020, European Union, through the European Regional Development Fund. FCT is also acknowledged for the grant attributed to J.S. (SFRH/BD/103255/2014).
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spelling Seaweeds’ neuroprotective potential set in vitro on a human cellular stress modelAntioxidantOxidative stressMitochondrial potentialApoptosisParkinson’s diseaseMarine natural compoundsAlgaeFunding This work was supported by the Portuguese Foundation for Science and Technology (FCT) through strategic project UID/MAR/04292/2020 granted to MARE—Marine and Environmental Sciences Centre, through Red2Discovery project (PTDC/MAR-BIO/6149/2014), co-fnanced by COMPETE (POCI-01-0145-FEDER-016791), through Oncologia de Precisão: Terapias e Tecnologias Inovadoras project (POINT4PAC) (SAICTPAC/0019/2015-LISBOA-01-0145-FEDER-016405) and through CrossAtlantic project (PTDC/BIA-OUT/29250/2017), co-fnanced by COMPETE (POCI-01-0145-FEDER-029250). This work was also funded by the Integrated Programme of SR&TD Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate (reference Centro-01-0145-FEDER-000018), co-funded by Centro 2020 Programme, Portugal 2020, European Union, through the European Regional Development Fund. FCT is also acknowledged for the grant attributed to J.S. (SFRH/BD/103255/2014).Neurodegenerative diseases, such as Parkinson’s disease, represent a biggest challenge for medicine, imposing high social and economic impacts. As a result, it is of utmost importance to develop new therapeutic strategies. The present work evaluated the neuroprotective potential of seaweeds extracts on an in vitro dopamine (DA)-induced neurotoxicity cellular model. The neuroprotective effects on SH-SY5Y cells’ viability were estimated by the MTT assay. Changes in mitochondrial membrane potential (MMP), caspase-3 activity, and hydrogen peroxide (H2O2) production were determined. DA (30–3000 μM; 24 h) treatment decreased SH-SY5Y cells’ viability in concentration and time-dependent manner, increasing the H2O2 production, MMP depolarization, and caspase-3 activity. On the other hand, DA (1000 μM; 24 h) toxicity was reduced (10–15%) with Sargassum muticum and Codium tomentosum extracts (1000 μg/mL; 24 h). The highest neuroprotective activity was exhibited by a methanolic extract obtained from Saccorhiza polyschides, which completely blunted DA effects. Results show that the marine seaweed S. polyschides contain substances with high neuroprotective potential against the toxicity induced by DA, exhibiting anti-apoptotic effects associated with both mitochondrial protection and caspase-3 inhibition. S. polyschides reveals, therefore, to be an excellent source of bioactive molecules, for new drugs development aiming PD therapeutics.SpringerIC-OnlineSilva, JoanaAlves, CelsoPinteus, SuseteMendes, SusanaPedrosa, Rui2020-08-28T14:09:50Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/5117engSilva, J. Alves, C., Pinteus, S., Mendes, S., & Pedrosa, R. (2020). Seaweeds’ neuroprotective potential set in vitro on a human cellular stress model. Molecular and Cellular Biochemistry. DOI:10.1007/s11010-020-03824-5.0300-817710.1007/s11010-020-03824-5metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:50:40Zoai:iconline.ipleiria.pt:10400.8/5117Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:48:47.637377Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Seaweeds’ neuroprotective potential set in vitro on a human cellular stress model
title Seaweeds’ neuroprotective potential set in vitro on a human cellular stress model
spellingShingle Seaweeds’ neuroprotective potential set in vitro on a human cellular stress model
Silva, Joana
Antioxidant
Oxidative stress
Mitochondrial potential
Apoptosis
Parkinson’s disease
Marine natural compounds
Algae
title_short Seaweeds’ neuroprotective potential set in vitro on a human cellular stress model
title_full Seaweeds’ neuroprotective potential set in vitro on a human cellular stress model
title_fullStr Seaweeds’ neuroprotective potential set in vitro on a human cellular stress model
title_full_unstemmed Seaweeds’ neuroprotective potential set in vitro on a human cellular stress model
title_sort Seaweeds’ neuroprotective potential set in vitro on a human cellular stress model
author Silva, Joana
author_facet Silva, Joana
Alves, Celso
Pinteus, Susete
Mendes, Susana
Pedrosa, Rui
author_role author
author2 Alves, Celso
Pinteus, Susete
Mendes, Susana
Pedrosa, Rui
author2_role author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Silva, Joana
Alves, Celso
Pinteus, Susete
Mendes, Susana
Pedrosa, Rui
dc.subject.por.fl_str_mv Antioxidant
Oxidative stress
Mitochondrial potential
Apoptosis
Parkinson’s disease
Marine natural compounds
Algae
topic Antioxidant
Oxidative stress
Mitochondrial potential
Apoptosis
Parkinson’s disease
Marine natural compounds
Algae
description Funding This work was supported by the Portuguese Foundation for Science and Technology (FCT) through strategic project UID/MAR/04292/2020 granted to MARE—Marine and Environmental Sciences Centre, through Red2Discovery project (PTDC/MAR-BIO/6149/2014), co-fnanced by COMPETE (POCI-01-0145-FEDER-016791), through Oncologia de Precisão: Terapias e Tecnologias Inovadoras project (POINT4PAC) (SAICTPAC/0019/2015-LISBOA-01-0145-FEDER-016405) and through CrossAtlantic project (PTDC/BIA-OUT/29250/2017), co-fnanced by COMPETE (POCI-01-0145-FEDER-029250). This work was also funded by the Integrated Programme of SR&TD Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate (reference Centro-01-0145-FEDER-000018), co-funded by Centro 2020 Programme, Portugal 2020, European Union, through the European Regional Development Fund. FCT is also acknowledged for the grant attributed to J.S. (SFRH/BD/103255/2014).
publishDate 2020
dc.date.none.fl_str_mv 2020-08-28T14:09:50Z
2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/5117
url http://hdl.handle.net/10400.8/5117
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva, J. Alves, C., Pinteus, S., Mendes, S., & Pedrosa, R. (2020). Seaweeds’ neuroprotective potential set in vitro on a human cellular stress model. Molecular and Cellular Biochemistry. DOI:10.1007/s11010-020-03824-5.
0300-8177
10.1007/s11010-020-03824-5
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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