Comparison of ISO 6579–1, VIDAS Easy SLM, and SureFast® Salmonella ONE Real-time PCR, for Salmonella Detection in Different Groups of Foodstuffs

Detalhes bibliográficos
Autor(a) principal: Furtado, R.
Data de Publicação: 2021
Outros Autores: Coelho, A., Morais, M., Leitão, A.L., Saraiva, M., Correia, C.B., Batista, R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/7824
Resumo: We thank AMBIFOOD, Lda, for providing the reagent kits and all the necessary equipment for the PCR methodolog.
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spelling Comparison of ISO 6579–1, VIDAS Easy SLM, and SureFast® Salmonella ONE Real-time PCR, for Salmonella Detection in Different Groups of FoodstuffsFoodstufsSalmonella Detection ·Conventional and Alternative MethodsFood SafetySalmonella sppSegurança AlimentarComposição dos AlimentosWe thank AMBIFOOD, Lda, for providing the reagent kits and all the necessary equipment for the PCR methodolog.The online version contains supplementary material available at https://doi.org/10.1007/s12161-021-02114-0.In the European Union (EU), Salmonella was the causative agent responsible for almost one in three (30.7%) of all foodborne outbreaks reported by member states during 2018, causing 11,581 cases of illness, which represented an increase of 20.6% compared to 2017. Considering the importance of this foodborne zoonotic bacterium in food safety and human health, several strategies for the control and consequent detection of Salmonella in foodstuffs are continuously being developed. In this study, we have tested 137 food samples (78 potentially naturally contaminated, 21 artificially contaminated with high levels of Salmonella, and 38 artificially contaminated with low levels of Salmonella) in order to compare the results and performance of three Salmonella detection methods: standard conventional culture (ISO 6579–1), SureFast® Salmonella ONE real-time PCR, and VIDAS® (Vitek Immunodiagnostic Assay System) Easy SLM, an Enzyme Linked Fluorescent Assay (ELFA). Although SureFast® Salmonella ONE real-time PCR was the fastest, it showed more inconclusive results, due to PCR inhibition and false positive results. ISO and VIDAS® protocols gave identical results and proved to be more robust than SureFast® Salmonella ONE real-time PCR when testing different food matrices, despite its longer response times. SureFast® Salmonella ONE real-time PCR may be appropriate to be used when the objective is to test food matrices that are known not to interfere with PCR and expected to be negative for Salmonella. All the analytical tested methods have advantages and limitations and thus, depending on the situation, may be used as the elected method for Salmonella detection in foodstuffs in accordance with the purpose of the laboratorial analysis.This work was funded by Instituto Nacional de Saúde Dr. Ricardo JorgeSpringerRepositório Científico do Instituto Nacional de SaúdeFurtado, R.Coelho, A.Morais, M.Leitão, A.L.Saraiva, M.Correia, C.B.Batista, R.2021-12-20T16:12:50Z2021-09-072021-09-07T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7824engFood Anal Methods. 2021. Epub 07 September 2021. doi: 10.1007/s12161-021-02114-01936-975110.1007/s12161-021-02114-01936-976Xinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:17Zoai:repositorio.insa.pt:10400.18/7824Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:33.350008Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Comparison of ISO 6579–1, VIDAS Easy SLM, and SureFast® Salmonella ONE Real-time PCR, for Salmonella Detection in Different Groups of Foodstuffs
title Comparison of ISO 6579–1, VIDAS Easy SLM, and SureFast® Salmonella ONE Real-time PCR, for Salmonella Detection in Different Groups of Foodstuffs
spellingShingle Comparison of ISO 6579–1, VIDAS Easy SLM, and SureFast® Salmonella ONE Real-time PCR, for Salmonella Detection in Different Groups of Foodstuffs
Furtado, R.
Foodstufs
Salmonella Detection ·
Conventional and Alternative Methods
Food Safety
Salmonella spp
Segurança Alimentar
Composição dos Alimentos
title_short Comparison of ISO 6579–1, VIDAS Easy SLM, and SureFast® Salmonella ONE Real-time PCR, for Salmonella Detection in Different Groups of Foodstuffs
title_full Comparison of ISO 6579–1, VIDAS Easy SLM, and SureFast® Salmonella ONE Real-time PCR, for Salmonella Detection in Different Groups of Foodstuffs
title_fullStr Comparison of ISO 6579–1, VIDAS Easy SLM, and SureFast® Salmonella ONE Real-time PCR, for Salmonella Detection in Different Groups of Foodstuffs
title_full_unstemmed Comparison of ISO 6579–1, VIDAS Easy SLM, and SureFast® Salmonella ONE Real-time PCR, for Salmonella Detection in Different Groups of Foodstuffs
title_sort Comparison of ISO 6579–1, VIDAS Easy SLM, and SureFast® Salmonella ONE Real-time PCR, for Salmonella Detection in Different Groups of Foodstuffs
author Furtado, R.
author_facet Furtado, R.
Coelho, A.
Morais, M.
Leitão, A.L.
Saraiva, M.
Correia, C.B.
Batista, R.
author_role author
author2 Coelho, A.
Morais, M.
Leitão, A.L.
Saraiva, M.
Correia, C.B.
Batista, R.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Furtado, R.
Coelho, A.
Morais, M.
Leitão, A.L.
Saraiva, M.
Correia, C.B.
Batista, R.
dc.subject.por.fl_str_mv Foodstufs
Salmonella Detection ·
Conventional and Alternative Methods
Food Safety
Salmonella spp
Segurança Alimentar
Composição dos Alimentos
topic Foodstufs
Salmonella Detection ·
Conventional and Alternative Methods
Food Safety
Salmonella spp
Segurança Alimentar
Composição dos Alimentos
description We thank AMBIFOOD, Lda, for providing the reagent kits and all the necessary equipment for the PCR methodolog.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-20T16:12:50Z
2021-09-07
2021-09-07T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/7824
url http://hdl.handle.net/10400.18/7824
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Food Anal Methods. 2021. Epub 07 September 2021. doi: 10.1007/s12161-021-02114-0
1936-9751
10.1007/s12161-021-02114-0
1936-976X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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