Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.12/1688 |
Resumo: | The rice semidwarfing gene, sd1, also known as the “green revolution gene”, has been studied intensively due to its contribution to the increase of crop production. Although sd1 breeding was extensively applied since the 1960s, the recent advances in the molecular basis of this gene allowed designing a more precise breeding strategy - marker assisted backcrossing (MAB) - to track sd1 introgression in two traditional rice varieties. For selection of sd1 plants we first confirmed the efficiency of specific markers based on Os200 x 2 gene sequence. Background selection was also performed with the help of microsatellites markers (SSR) and a total of 7 breeding lines were recovered containing a higher percentage of recurrent parent genome (RPG). Analysis of Covariance (ANCOVA) using mean progenitor plant height as covariate was performed to compare several agronomic and quality-related parameters in two different environments. The results suggest that plant height differs significantly between the two environments F(1, 220) = 155.336; p < 0.001. From the total variability of plant height we could conclude that 73% is due to the genotype, while 10.4% depends on the environment. In addition, the percentage of RPG seems negatively correlated with plant height (p < 0.005). MAB and background selection thus revealed as useful tools to assist breeding for semidwarfism in traditional rice varieties. |
id |
RCAP_a1f193bf300a3fb0b6efea2f8fa425d7 |
---|---|
oai_identifier_str |
oai:repositorio.ispa.pt:10400.12/1688 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-studyRiceSemidwarfismSd1Environmental influenceThe rice semidwarfing gene, sd1, also known as the “green revolution gene”, has been studied intensively due to its contribution to the increase of crop production. Although sd1 breeding was extensively applied since the 1960s, the recent advances in the molecular basis of this gene allowed designing a more precise breeding strategy - marker assisted backcrossing (MAB) - to track sd1 introgression in two traditional rice varieties. For selection of sd1 plants we first confirmed the efficiency of specific markers based on Os200 x 2 gene sequence. Background selection was also performed with the help of microsatellites markers (SSR) and a total of 7 breeding lines were recovered containing a higher percentage of recurrent parent genome (RPG). Analysis of Covariance (ANCOVA) using mean progenitor plant height as covariate was performed to compare several agronomic and quality-related parameters in two different environments. The results suggest that plant height differs significantly between the two environments F(1, 220) = 155.336; p < 0.001. From the total variability of plant height we could conclude that 73% is due to the genotype, while 10.4% depends on the environment. In addition, the percentage of RPG seems negatively correlated with plant height (p < 0.005). MAB and background selection thus revealed as useful tools to assist breeding for semidwarfism in traditional rice varieties.Academic JournalsRepositório do ISPANegrão, SóniaPalaniappan, JayamaniMaroco, JoãoLourenço, TiagoMackill, DavidOliveira, Maria Margarida2012-09-11T18:07:25Z2010-01-01T00:00:00Z2010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.12/1688engAfrican Journal of Biotechnology, 9(15), 2192-22001684–5315info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-05T16:37:42Zoai:repositorio.ispa.pt:10400.12/1688Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:19:45.462168Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study |
title |
Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study |
spellingShingle |
Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study Negrão, Sónia Rice Semidwarfism Sd1 Environmental influence |
title_short |
Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study |
title_full |
Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study |
title_fullStr |
Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study |
title_full_unstemmed |
Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study |
title_sort |
Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study |
author |
Negrão, Sónia |
author_facet |
Negrão, Sónia Palaniappan, Jayamani Maroco, João Lourenço, Tiago Mackill, David Oliveira, Maria Margarida |
author_role |
author |
author2 |
Palaniappan, Jayamani Maroco, João Lourenço, Tiago Mackill, David Oliveira, Maria Margarida |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório do ISPA |
dc.contributor.author.fl_str_mv |
Negrão, Sónia Palaniappan, Jayamani Maroco, João Lourenço, Tiago Mackill, David Oliveira, Maria Margarida |
dc.subject.por.fl_str_mv |
Rice Semidwarfism Sd1 Environmental influence |
topic |
Rice Semidwarfism Sd1 Environmental influence |
description |
The rice semidwarfing gene, sd1, also known as the “green revolution gene”, has been studied intensively due to its contribution to the increase of crop production. Although sd1 breeding was extensively applied since the 1960s, the recent advances in the molecular basis of this gene allowed designing a more precise breeding strategy - marker assisted backcrossing (MAB) - to track sd1 introgression in two traditional rice varieties. For selection of sd1 plants we first confirmed the efficiency of specific markers based on Os200 x 2 gene sequence. Background selection was also performed with the help of microsatellites markers (SSR) and a total of 7 breeding lines were recovered containing a higher percentage of recurrent parent genome (RPG). Analysis of Covariance (ANCOVA) using mean progenitor plant height as covariate was performed to compare several agronomic and quality-related parameters in two different environments. The results suggest that plant height differs significantly between the two environments F(1, 220) = 155.336; p < 0.001. From the total variability of plant height we could conclude that 73% is due to the genotype, while 10.4% depends on the environment. In addition, the percentage of RPG seems negatively correlated with plant height (p < 0.005). MAB and background selection thus revealed as useful tools to assist breeding for semidwarfism in traditional rice varieties. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01T00:00:00Z 2010-01-01T00:00:00Z 2012-09-11T18:07:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.12/1688 |
url |
http://hdl.handle.net/10400.12/1688 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
African Journal of Biotechnology, 9(15), 2192-2200 1684–5315 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Academic Journals |
publisher.none.fl_str_mv |
Academic Journals |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799130058215915520 |