In vivo biofunctional evaluation of hydrogels for disc regeneration
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/26928 |
Resumo: | Purpose Regenerative strategies aim to restore the original biofunctionality of the intervertebral disc. Different biomaterials are available, which might support disc regeneration. In the present study, the prospects of success of two hydrogels functionalized with anti-angiogenic peptides and seeded with bone marrow derived mononuclear cells (BMC), respectively, were investigated in an ovine nucleotomy model. Methods In a one-step procedure iliac crest aspirates were harvested and, subsequently, separated BMC were seeded on hydrogels and implanted into the ovine disc. For the cell-seeded approach a hyaluronic acid-based hydrogel was used. The anti-angiogenic potential of newly developed VEGF-blockers was investigated on ionically crosslinked metacrylated gellan gum hydrogels. Untreated discs served as nucleotomy controls. 24 adult merino sheep were used. After 6 weeks histological, after 12 weeks histological and biomechanical analyses were conducted. Results Biomechanical tests revealed no differences between any of the implanted and nucleotomized discs. All implanted discs significantly degenerated compared to intact discs. In contrast, there was no marked difference between implanted and nucleotomized discs. In tendency, albeit not significant, degeneration score and disc height index deteriorated for all but not for the cell-seeded hydrogels from 6 to 12 weeks. Cell-seeded hydrogels slightly decelerated degeneration. Conclusions None of the hydrogel configurations was able to regenerate biofunctionality of the intervertebral disc. This might presumably be caused by hydrogel extrusion. Great importance should be given to the development of annulus sealants, which effectively exploit the potential of (cell-seeded) hydrogels for biological disc regeneration and restoration of intervertebral disc functioning |
id |
RCAP_a23819c7721a0206c0f57651c6d7af43 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/26928 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
In vivo biofunctional evaluation of hydrogels for disc regenerationDegenerationIn vivoIntervertebral discLarge animal modelOne-stepRegenerationSheepScience & TechnologyPurpose Regenerative strategies aim to restore the original biofunctionality of the intervertebral disc. Different biomaterials are available, which might support disc regeneration. In the present study, the prospects of success of two hydrogels functionalized with anti-angiogenic peptides and seeded with bone marrow derived mononuclear cells (BMC), respectively, were investigated in an ovine nucleotomy model. Methods In a one-step procedure iliac crest aspirates were harvested and, subsequently, separated BMC were seeded on hydrogels and implanted into the ovine disc. For the cell-seeded approach a hyaluronic acid-based hydrogel was used. The anti-angiogenic potential of newly developed VEGF-blockers was investigated on ionically crosslinked metacrylated gellan gum hydrogels. Untreated discs served as nucleotomy controls. 24 adult merino sheep were used. After 6 weeks histological, after 12 weeks histological and biomechanical analyses were conducted. Results Biomechanical tests revealed no differences between any of the implanted and nucleotomized discs. All implanted discs significantly degenerated compared to intact discs. In contrast, there was no marked difference between implanted and nucleotomized discs. In tendency, albeit not significant, degeneration score and disc height index deteriorated for all but not for the cell-seeded hydrogels from 6 to 12 weeks. Cell-seeded hydrogels slightly decelerated degeneration. Conclusions None of the hydrogel configurations was able to regenerate biofunctionality of the intervertebral disc. This might presumably be caused by hydrogel extrusion. Great importance should be given to the development of annulus sealants, which effectively exploit the potential of (cell-seeded) hydrogels for biological disc regeneration and restoration of intervertebral disc functioningThis work was supported by the EU-project Disc Regeneration (NMP3-LA-2008-213904). Technical assistance of Iris Baum and the whole animal surgery team of the Institute of Orthopaedic Research and Biomechanics, Ulm, are gratefully acknowledged. DDAHA hydrogels were kindly provided by Cristina Longinotti (DDAHA, Anika Therapeutics, Abano Therme, Italy).SpringerUniversidade do MinhoReitmaier, SandraKreja, LudwikaGruchenberg, KatharinaKanter, BrittaSilva-Correia, JoanaOliveira, Joaquim M.Reis, R. L.Perugini, V.Santin, M.Ignatius, AnitaWilke, Hans-Joachim20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/26928engReitmaier S., Kreja L., Gruchenberg K., Kanter B., Silva-Correia J., Oliveira J. M., Reis R. L., Perugini V., Santin M., Ignatius A., Wilke H. - J. In vivo biofunctional evaluation of hydrogels for disc regeneration, European Spine Journal, doi:10.1007/s00586-013-2998-8, 2014.0940-671910.1007/s00586-013-2998-824121748The original publication is available at www.springerlink.cominfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:01:38Zoai:repositorium.sdum.uminho.pt:1822/26928Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:34.061537Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
In vivo biofunctional evaluation of hydrogels for disc regeneration |
title |
In vivo biofunctional evaluation of hydrogels for disc regeneration |
spellingShingle |
In vivo biofunctional evaluation of hydrogels for disc regeneration Reitmaier, Sandra Degeneration In vivo Intervertebral disc Large animal model One-step Regeneration Sheep Science & Technology |
title_short |
In vivo biofunctional evaluation of hydrogels for disc regeneration |
title_full |
In vivo biofunctional evaluation of hydrogels for disc regeneration |
title_fullStr |
In vivo biofunctional evaluation of hydrogels for disc regeneration |
title_full_unstemmed |
In vivo biofunctional evaluation of hydrogels for disc regeneration |
title_sort |
In vivo biofunctional evaluation of hydrogels for disc regeneration |
author |
Reitmaier, Sandra |
author_facet |
Reitmaier, Sandra Kreja, Ludwika Gruchenberg, Katharina Kanter, Britta Silva-Correia, Joana Oliveira, Joaquim M. Reis, R. L. Perugini, V. Santin, M. Ignatius, Anita Wilke, Hans-Joachim |
author_role |
author |
author2 |
Kreja, Ludwika Gruchenberg, Katharina Kanter, Britta Silva-Correia, Joana Oliveira, Joaquim M. Reis, R. L. Perugini, V. Santin, M. Ignatius, Anita Wilke, Hans-Joachim |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Reitmaier, Sandra Kreja, Ludwika Gruchenberg, Katharina Kanter, Britta Silva-Correia, Joana Oliveira, Joaquim M. Reis, R. L. Perugini, V. Santin, M. Ignatius, Anita Wilke, Hans-Joachim |
dc.subject.por.fl_str_mv |
Degeneration In vivo Intervertebral disc Large animal model One-step Regeneration Sheep Science & Technology |
topic |
Degeneration In vivo Intervertebral disc Large animal model One-step Regeneration Sheep Science & Technology |
description |
Purpose Regenerative strategies aim to restore the original biofunctionality of the intervertebral disc. Different biomaterials are available, which might support disc regeneration. In the present study, the prospects of success of two hydrogels functionalized with anti-angiogenic peptides and seeded with bone marrow derived mononuclear cells (BMC), respectively, were investigated in an ovine nucleotomy model. Methods In a one-step procedure iliac crest aspirates were harvested and, subsequently, separated BMC were seeded on hydrogels and implanted into the ovine disc. For the cell-seeded approach a hyaluronic acid-based hydrogel was used. The anti-angiogenic potential of newly developed VEGF-blockers was investigated on ionically crosslinked metacrylated gellan gum hydrogels. Untreated discs served as nucleotomy controls. 24 adult merino sheep were used. After 6 weeks histological, after 12 weeks histological and biomechanical analyses were conducted. Results Biomechanical tests revealed no differences between any of the implanted and nucleotomized discs. All implanted discs significantly degenerated compared to intact discs. In contrast, there was no marked difference between implanted and nucleotomized discs. In tendency, albeit not significant, degeneration score and disc height index deteriorated for all but not for the cell-seeded hydrogels from 6 to 12 weeks. Cell-seeded hydrogels slightly decelerated degeneration. Conclusions None of the hydrogel configurations was able to regenerate biofunctionality of the intervertebral disc. This might presumably be caused by hydrogel extrusion. Great importance should be given to the development of annulus sealants, which effectively exploit the potential of (cell-seeded) hydrogels for biological disc regeneration and restoration of intervertebral disc functioning |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/26928 |
url |
http://hdl.handle.net/1822/26928 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reitmaier S., Kreja L., Gruchenberg K., Kanter B., Silva-Correia J., Oliveira J. M., Reis R. L., Perugini V., Santin M., Ignatius A., Wilke H. - J. In vivo biofunctional evaluation of hydrogels for disc regeneration, European Spine Journal, doi:10.1007/s00586-013-2998-8, 2014. 0940-6719 10.1007/s00586-013-2998-8 24121748 The original publication is available at www.springerlink.com |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132288903020544 |