Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymes
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/17813 |
Resumo: | Eugenol is a phenolic compound largely found in the clove essential oil that possesses promising biological activity. However, its low water solubility is a major concern and encapsulation is an alternative to improve water affinity. The objective of this work was to produce nanostructured lipid carriers (NLC) by hot homogenization/ultrasound emulsification and to evaluate the effect of free and encapsulated clove oil on the in vitro cholinesterase enzymes modulation using Drosophila melanogaster (DM) tissue. The NLC composed of a natural wax (carnauba or beeswax) and crodamol showed an average diameter between 121 and 367 nm with good dispersion and colloidal stability. The spherical shape and solid character together with the semi-crystalline environment confirm the formation of NLC. DSC analysis indicated polymorphic transition events of the waxes. In vitro tests using DM demonstrated that free clove oil showed a good inhibition of the butyryl and acetylcholinesterase enzymes above a concentration of 10 mM, with IC50 values of 4.3 and 3.5 mM, respectively. The dispersions of the NLC loaded with clove oil showed a decrease in the IC50 enzymes values, indicating the preservation of the clove essential oil and suggesting an increased in the solubility. Results indicate that NLC dispersions have good potential to be used for foods and cosmetic aqueous formulations possessing biological activity. |
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Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymesAcetylcholinesteraseBeeswaxButyrylcholinesteraseCarnauba waxClove oilDrosophila melanogasterEncapsulationEugenol is a phenolic compound largely found in the clove essential oil that possesses promising biological activity. However, its low water solubility is a major concern and encapsulation is an alternative to improve water affinity. The objective of this work was to produce nanostructured lipid carriers (NLC) by hot homogenization/ultrasound emulsification and to evaluate the effect of free and encapsulated clove oil on the in vitro cholinesterase enzymes modulation using Drosophila melanogaster (DM) tissue. The NLC composed of a natural wax (carnauba or beeswax) and crodamol showed an average diameter between 121 and 367 nm with good dispersion and colloidal stability. The spherical shape and solid character together with the semi-crystalline environment confirm the formation of NLC. DSC analysis indicated polymorphic transition events of the waxes. In vitro tests using DM demonstrated that free clove oil showed a good inhibition of the butyryl and acetylcholinesterase enzymes above a concentration of 10 mM, with IC50 values of 4.3 and 3.5 mM, respectively. The dispersions of the NLC loaded with clove oil showed a decrease in the IC50 enzymes values, indicating the preservation of the clove essential oil and suggesting an increased in the solubility. Results indicate that NLC dispersions have good potential to be used for foods and cosmetic aqueous formulations possessing biological activity.Authors thank the financial support from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), and also Laboratório Central de Microscopia Eletrônica of Universidade Federal de Santa Catarina (LCME/UFSC) for TEM analyses.Biblioteca Digital do IPBMeneses, Alessandra Cristina deMarques, Elisa Balbi PintoLeimann, Fernanda VitóriaGonçalves, Odinei HessIneu, Rafael P.Araújo, Pedro Henrique Hermes deOliveira, Débora deSayer, Claudia2018-01-19T10:00:00Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/17813engMeneses, Alessandra Cristina; Marques, Elisa Balbi Pinto; Leimann, Fernanda Vitória; Gonçalves, Odinei Hess; Ineu, Rafael Porto; de Araújo, Pedro Henrique Hermes; de Oliveira, Débora; Sayer, Claudia (2019). Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymes. Colloids and Surfaces A: Physicochemical and Engineering Aspects. ISSN 0927-7757. 583, p. 1-80927-775710.1016/j.colsurfa.2019.123879info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:45:49Zoai:bibliotecadigital.ipb.pt:10198/17813Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:10:49.477624Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymes |
title |
Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymes |
spellingShingle |
Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymes Meneses, Alessandra Cristina de Acetylcholinesterase Beeswax Butyrylcholinesterase Carnauba wax Clove oil Drosophila melanogaster Encapsulation |
title_short |
Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymes |
title_full |
Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymes |
title_fullStr |
Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymes |
title_full_unstemmed |
Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymes |
title_sort |
Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymes |
author |
Meneses, Alessandra Cristina de |
author_facet |
Meneses, Alessandra Cristina de Marques, Elisa Balbi Pinto Leimann, Fernanda Vitória Gonçalves, Odinei Hess Ineu, Rafael P. Araújo, Pedro Henrique Hermes de Oliveira, Débora de Sayer, Claudia |
author_role |
author |
author2 |
Marques, Elisa Balbi Pinto Leimann, Fernanda Vitória Gonçalves, Odinei Hess Ineu, Rafael P. Araújo, Pedro Henrique Hermes de Oliveira, Débora de Sayer, Claudia |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Meneses, Alessandra Cristina de Marques, Elisa Balbi Pinto Leimann, Fernanda Vitória Gonçalves, Odinei Hess Ineu, Rafael P. Araújo, Pedro Henrique Hermes de Oliveira, Débora de Sayer, Claudia |
dc.subject.por.fl_str_mv |
Acetylcholinesterase Beeswax Butyrylcholinesterase Carnauba wax Clove oil Drosophila melanogaster Encapsulation |
topic |
Acetylcholinesterase Beeswax Butyrylcholinesterase Carnauba wax Clove oil Drosophila melanogaster Encapsulation |
description |
Eugenol is a phenolic compound largely found in the clove essential oil that possesses promising biological activity. However, its low water solubility is a major concern and encapsulation is an alternative to improve water affinity. The objective of this work was to produce nanostructured lipid carriers (NLC) by hot homogenization/ultrasound emulsification and to evaluate the effect of free and encapsulated clove oil on the in vitro cholinesterase enzymes modulation using Drosophila melanogaster (DM) tissue. The NLC composed of a natural wax (carnauba or beeswax) and crodamol showed an average diameter between 121 and 367 nm with good dispersion and colloidal stability. The spherical shape and solid character together with the semi-crystalline environment confirm the formation of NLC. DSC analysis indicated polymorphic transition events of the waxes. In vitro tests using DM demonstrated that free clove oil showed a good inhibition of the butyryl and acetylcholinesterase enzymes above a concentration of 10 mM, with IC50 values of 4.3 and 3.5 mM, respectively. The dispersions of the NLC loaded with clove oil showed a decrease in the IC50 enzymes values, indicating the preservation of the clove essential oil and suggesting an increased in the solubility. Results indicate that NLC dispersions have good potential to be used for foods and cosmetic aqueous formulations possessing biological activity. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-19T10:00:00Z 2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/17813 |
url |
http://hdl.handle.net/10198/17813 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Meneses, Alessandra Cristina; Marques, Elisa Balbi Pinto; Leimann, Fernanda Vitória; Gonçalves, Odinei Hess; Ineu, Rafael Porto; de Araújo, Pedro Henrique Hermes; de Oliveira, Débora; Sayer, Claudia (2019). Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymes. Colloids and Surfaces A: Physicochemical and Engineering Aspects. ISSN 0927-7757. 583, p. 1-8 0927-7757 10.1016/j.colsurfa.2019.123879 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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