PLGA nanoparticles as a platform for vitamin D-based cancer therapy

Detalhes bibliográficos
Autor(a) principal: M. Carmo Pereira
Data de Publicação: 2015
Outros Autores: Maria J. Ramalho, Joana A. Loureiro, Manuela F. Frasco, Manuel A. N. Coelho
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/103083
Resumo: Poly(lactic-co-glycolic acid) (PLGA) nanoparticles were studied as drug delivery vehicles for calcitriol, the active form of vitamin D-3. In vitro effects of calcitriol encapsulated in PLGA nanoparticles were evaluated with respect to free calcitriol on human pancreatic cell lines, S2-013 and hTERT-HPNE, and the lung cancer cell line A549. Encapsulated calcitriol retained its biological activity, reducing the cell growth. Cytotoxicity assays demonstrated that encapsulation of calcitriol enhanced its inhibitory effect on cell growth at a concentration of 2.4 mu M for the S2-013 cells (91%) and for A549 cells (70%) comparared to the free calcitriol results. At this concentration the inhibitory effect on nontumor cells (hTERT-HPNE) decreased to 65%. This study highlights the ability of PLGA nanoparticles to deliver vitamin D-3 into cancer cells, with major effects regarding cancer cell cycle arrest and major changes in the cell morphological features.
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spelling PLGA nanoparticles as a platform for vitamin D-based cancer therapyCiências da Saúde, Ciências Tecnológicas, Ciências da engenharia e tecnologias, Ciências médicas e da saúdeHealth sciences, Technological sciences, Engineering and technology, Medical and Health sciencesPoly(lactic-co-glycolic acid) (PLGA) nanoparticles were studied as drug delivery vehicles for calcitriol, the active form of vitamin D-3. In vitro effects of calcitriol encapsulated in PLGA nanoparticles were evaluated with respect to free calcitriol on human pancreatic cell lines, S2-013 and hTERT-HPNE, and the lung cancer cell line A549. Encapsulated calcitriol retained its biological activity, reducing the cell growth. Cytotoxicity assays demonstrated that encapsulation of calcitriol enhanced its inhibitory effect on cell growth at a concentration of 2.4 mu M for the S2-013 cells (91%) and for A549 cells (70%) comparared to the free calcitriol results. At this concentration the inhibitory effect on nontumor cells (hTERT-HPNE) decreased to 65%. This study highlights the ability of PLGA nanoparticles to deliver vitamin D-3 into cancer cells, with major effects regarding cancer cell cycle arrest and major changes in the cell morphological features.20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/103083eng2190-428610.3762/bjnano.6.135M. Carmo PereiraMaria J. RamalhoJoana A. LoureiroManuela F. FrascoManuel A. N. Coelhoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T15:03:23Zoai:repositorio-aberto.up.pt:10216/103083Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:14:35.990087Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv PLGA nanoparticles as a platform for vitamin D-based cancer therapy
title PLGA nanoparticles as a platform for vitamin D-based cancer therapy
spellingShingle PLGA nanoparticles as a platform for vitamin D-based cancer therapy
M. Carmo Pereira
Ciências da Saúde, Ciências Tecnológicas, Ciências da engenharia e tecnologias, Ciências médicas e da saúde
Health sciences, Technological sciences, Engineering and technology, Medical and Health sciences
title_short PLGA nanoparticles as a platform for vitamin D-based cancer therapy
title_full PLGA nanoparticles as a platform for vitamin D-based cancer therapy
title_fullStr PLGA nanoparticles as a platform for vitamin D-based cancer therapy
title_full_unstemmed PLGA nanoparticles as a platform for vitamin D-based cancer therapy
title_sort PLGA nanoparticles as a platform for vitamin D-based cancer therapy
author M. Carmo Pereira
author_facet M. Carmo Pereira
Maria J. Ramalho
Joana A. Loureiro
Manuela F. Frasco
Manuel A. N. Coelho
author_role author
author2 Maria J. Ramalho
Joana A. Loureiro
Manuela F. Frasco
Manuel A. N. Coelho
author2_role author
author
author
author
dc.contributor.author.fl_str_mv M. Carmo Pereira
Maria J. Ramalho
Joana A. Loureiro
Manuela F. Frasco
Manuel A. N. Coelho
dc.subject.por.fl_str_mv Ciências da Saúde, Ciências Tecnológicas, Ciências da engenharia e tecnologias, Ciências médicas e da saúde
Health sciences, Technological sciences, Engineering and technology, Medical and Health sciences
topic Ciências da Saúde, Ciências Tecnológicas, Ciências da engenharia e tecnologias, Ciências médicas e da saúde
Health sciences, Technological sciences, Engineering and technology, Medical and Health sciences
description Poly(lactic-co-glycolic acid) (PLGA) nanoparticles were studied as drug delivery vehicles for calcitriol, the active form of vitamin D-3. In vitro effects of calcitriol encapsulated in PLGA nanoparticles were evaluated with respect to free calcitriol on human pancreatic cell lines, S2-013 and hTERT-HPNE, and the lung cancer cell line A549. Encapsulated calcitriol retained its biological activity, reducing the cell growth. Cytotoxicity assays demonstrated that encapsulation of calcitriol enhanced its inhibitory effect on cell growth at a concentration of 2.4 mu M for the S2-013 cells (91%) and for A549 cells (70%) comparared to the free calcitriol results. At this concentration the inhibitory effect on nontumor cells (hTERT-HPNE) decreased to 65%. This study highlights the ability of PLGA nanoparticles to deliver vitamin D-3 into cancer cells, with major effects regarding cancer cell cycle arrest and major changes in the cell morphological features.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/103083
url https://hdl.handle.net/10216/103083
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2190-4286
10.3762/bjnano.6.135
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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