PLGA nanoparticles as a platform for vitamin D-based cancer therapy
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/10216/103083 |
Resumo: | Poly(lactic-co-glycolic acid) (PLGA) nanoparticles were studied as drug delivery vehicles for calcitriol, the active form of vitamin D-3. In vitro effects of calcitriol encapsulated in PLGA nanoparticles were evaluated with respect to free calcitriol on human pancreatic cell lines, S2-013 and hTERT-HPNE, and the lung cancer cell line A549. Encapsulated calcitriol retained its biological activity, reducing the cell growth. Cytotoxicity assays demonstrated that encapsulation of calcitriol enhanced its inhibitory effect on cell growth at a concentration of 2.4 mu M for the S2-013 cells (91%) and for A549 cells (70%) comparared to the free calcitriol results. At this concentration the inhibitory effect on nontumor cells (hTERT-HPNE) decreased to 65%. This study highlights the ability of PLGA nanoparticles to deliver vitamin D-3 into cancer cells, with major effects regarding cancer cell cycle arrest and major changes in the cell morphological features. |
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PLGA nanoparticles as a platform for vitamin D-based cancer therapyCiências da Saúde, Ciências Tecnológicas, Ciências da engenharia e tecnologias, Ciências médicas e da saúdeHealth sciences, Technological sciences, Engineering and technology, Medical and Health sciencesPoly(lactic-co-glycolic acid) (PLGA) nanoparticles were studied as drug delivery vehicles for calcitriol, the active form of vitamin D-3. In vitro effects of calcitriol encapsulated in PLGA nanoparticles were evaluated with respect to free calcitriol on human pancreatic cell lines, S2-013 and hTERT-HPNE, and the lung cancer cell line A549. Encapsulated calcitriol retained its biological activity, reducing the cell growth. Cytotoxicity assays demonstrated that encapsulation of calcitriol enhanced its inhibitory effect on cell growth at a concentration of 2.4 mu M for the S2-013 cells (91%) and for A549 cells (70%) comparared to the free calcitriol results. At this concentration the inhibitory effect on nontumor cells (hTERT-HPNE) decreased to 65%. This study highlights the ability of PLGA nanoparticles to deliver vitamin D-3 into cancer cells, with major effects regarding cancer cell cycle arrest and major changes in the cell morphological features.20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/103083eng2190-428610.3762/bjnano.6.135M. Carmo PereiraMaria J. RamalhoJoana A. LoureiroManuela F. FrascoManuel A. N. Coelhoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T15:03:23Zoai:repositorio-aberto.up.pt:10216/103083Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:14:35.990087Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
PLGA nanoparticles as a platform for vitamin D-based cancer therapy |
title |
PLGA nanoparticles as a platform for vitamin D-based cancer therapy |
spellingShingle |
PLGA nanoparticles as a platform for vitamin D-based cancer therapy M. Carmo Pereira Ciências da Saúde, Ciências Tecnológicas, Ciências da engenharia e tecnologias, Ciências médicas e da saúde Health sciences, Technological sciences, Engineering and technology, Medical and Health sciences |
title_short |
PLGA nanoparticles as a platform for vitamin D-based cancer therapy |
title_full |
PLGA nanoparticles as a platform for vitamin D-based cancer therapy |
title_fullStr |
PLGA nanoparticles as a platform for vitamin D-based cancer therapy |
title_full_unstemmed |
PLGA nanoparticles as a platform for vitamin D-based cancer therapy |
title_sort |
PLGA nanoparticles as a platform for vitamin D-based cancer therapy |
author |
M. Carmo Pereira |
author_facet |
M. Carmo Pereira Maria J. Ramalho Joana A. Loureiro Manuela F. Frasco Manuel A. N. Coelho |
author_role |
author |
author2 |
Maria J. Ramalho Joana A. Loureiro Manuela F. Frasco Manuel A. N. Coelho |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
M. Carmo Pereira Maria J. Ramalho Joana A. Loureiro Manuela F. Frasco Manuel A. N. Coelho |
dc.subject.por.fl_str_mv |
Ciências da Saúde, Ciências Tecnológicas, Ciências da engenharia e tecnologias, Ciências médicas e da saúde Health sciences, Technological sciences, Engineering and technology, Medical and Health sciences |
topic |
Ciências da Saúde, Ciências Tecnológicas, Ciências da engenharia e tecnologias, Ciências médicas e da saúde Health sciences, Technological sciences, Engineering and technology, Medical and Health sciences |
description |
Poly(lactic-co-glycolic acid) (PLGA) nanoparticles were studied as drug delivery vehicles for calcitriol, the active form of vitamin D-3. In vitro effects of calcitriol encapsulated in PLGA nanoparticles were evaluated with respect to free calcitriol on human pancreatic cell lines, S2-013 and hTERT-HPNE, and the lung cancer cell line A549. Encapsulated calcitriol retained its biological activity, reducing the cell growth. Cytotoxicity assays demonstrated that encapsulation of calcitriol enhanced its inhibitory effect on cell growth at a concentration of 2.4 mu M for the S2-013 cells (91%) and for A549 cells (70%) comparared to the free calcitriol results. At this concentration the inhibitory effect on nontumor cells (hTERT-HPNE) decreased to 65%. This study highlights the ability of PLGA nanoparticles to deliver vitamin D-3 into cancer cells, with major effects regarding cancer cell cycle arrest and major changes in the cell morphological features. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/10216/103083 |
url |
https://hdl.handle.net/10216/103083 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2190-4286 10.3762/bjnano.6.135 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136066959048705 |