Study and optimization of a microfluidic paper based analytical device for the quantification of salivary copper

Detalhes bibliográficos
Autor(a) principal: Almeida, Adriana Raquel Gomes Tavares
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/37945
Resumo: Early diagnosis of pathology can promote a better recovery and treatment. Thus, the development the devices the rapid evaluation of the health condition, contribute to this process. In this context, in the recent years there has been growth in the development of low cost, easy to use devices. In this work, a microfluidic paper-based analytical device (μPAD) was development for the determination of copper in human saliva, being a form of rapid test. The device is suitable for the detection of excess copper, as consequently to change the healthy status. The development of the device involved several studies in order to get the rapid determination, accurate, cheaper and easy to use. Colorimetric reaction was chosen between copper and diphenylcarbazide create the complex of red colour. After the reaction time has elapsed, a scanner is used to obtain the digital image of μPAD detection zone, in order to calculate the absorbance based on the intensity of colour of each analytical local, obtained by Image J software. This device provides a copper determination in the range of 80.0 – 600 μg/L, a calibration curve of A= (0.0701±0.0008) [Cu2+] – (0.0043 ± 0.0004), the limit of detection of 17 μg/L and the limit of quantification of 56 μg/L. After inserting the sample in the μPAD, it can be digitized, within a time period of 20 - 40 min. The accuracy of the results was assessed by calculating recovery percentages using saliva from volunteers.
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spelling Study and optimization of a microfluidic paper based analytical device for the quantification of salivary copperCopperµPADSalivaColorimetric reactionCobreReação colorimétricaDomínio/Área Científica::Engenharia e Tecnologia::Engenharia MédicaEarly diagnosis of pathology can promote a better recovery and treatment. Thus, the development the devices the rapid evaluation of the health condition, contribute to this process. In this context, in the recent years there has been growth in the development of low cost, easy to use devices. In this work, a microfluidic paper-based analytical device (μPAD) was development for the determination of copper in human saliva, being a form of rapid test. The device is suitable for the detection of excess copper, as consequently to change the healthy status. The development of the device involved several studies in order to get the rapid determination, accurate, cheaper and easy to use. Colorimetric reaction was chosen between copper and diphenylcarbazide create the complex of red colour. After the reaction time has elapsed, a scanner is used to obtain the digital image of μPAD detection zone, in order to calculate the absorbance based on the intensity of colour of each analytical local, obtained by Image J software. This device provides a copper determination in the range of 80.0 – 600 μg/L, a calibration curve of A= (0.0701±0.0008) [Cu2+] – (0.0043 ± 0.0004), the limit of detection of 17 μg/L and the limit of quantification of 56 μg/L. After inserting the sample in the μPAD, it can be digitized, within a time period of 20 - 40 min. The accuracy of the results was assessed by calculating recovery percentages using saliva from volunteers.O diagnóstico precoce de uma patologia permite promover um melhor tratamento e recuperação. Assim, o desenvolvimento de dispositivos de avaliação da condição de saúde de forma rápida, contribuem para este processo. Neste sentido, nos últimos anos tem havido um crescimento no desenvolvimento de dispositivos de baixo custo e que sejam fáceis de ser utilizados. Neste trabalho foi desenvolvido um dispositivo analítico baseado na análise por microfluxo em papel (μPAD) para a determinação de cobre em saliva humana, sendo uma forma de teste rápido. O dispositivo é adequado para a detenção de excesso de cobre como consequência de alteração na condição de saúde. O desenvolvimento do dispositivo envolveu vários estudos de modo a conseguir uma determinação rápida, precisa, de baixo custo e de fácil utilização. Foi escolhida a reação colorimétrica entre o cobre e a difenilcarbazida formando um complexo de cor vermelha. Após decorrer o tempo de reação, utiliza-se um digitalizador para obter uma imagem digital da zona de deteção do μPAD, de forma a calcular a absorvância com base na intensidade de cor de cada local de análise, obtida pela utilização do software Image J. O dispositivo desenvolvido, permite a determinação de cobre num intervalo de 80,0 –600 μg/L, com uma curva de calibração A= (0.0701±0.0008) [Cu2+] – (0.0043 ± 0.0004), um limite de deteção de 17 μg/L e o limite de quantificação de 56 μg/L. Após a aplicação das amostras no μPAD, a leitura da cor pode ser feita entre 20-40 min. A exatidão dos resultados foi aferida por calculo de percentagens de recuperação utilizando salivas de voluntários.Mesquita, Raquel Beatriz Ribeiro deRangel, António Osmaro Santos SilvaVeritati - Repositório Institucional da Universidade Católica PortuguesaAlmeida, Adriana Raquel Gomes Tavares2022-12-22T01:30:30Z2022-05-232022-042022-05-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.14/37945TID:203025202enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:43:27Zoai:repositorio.ucp.pt:10400.14/37945Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:30:55.300759Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Study and optimization of a microfluidic paper based analytical device for the quantification of salivary copper
title Study and optimization of a microfluidic paper based analytical device for the quantification of salivary copper
spellingShingle Study and optimization of a microfluidic paper based analytical device for the quantification of salivary copper
Almeida, Adriana Raquel Gomes Tavares
Copper
µPAD
Saliva
Colorimetric reaction
Cobre
Reação colorimétrica
Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Médica
title_short Study and optimization of a microfluidic paper based analytical device for the quantification of salivary copper
title_full Study and optimization of a microfluidic paper based analytical device for the quantification of salivary copper
title_fullStr Study and optimization of a microfluidic paper based analytical device for the quantification of salivary copper
title_full_unstemmed Study and optimization of a microfluidic paper based analytical device for the quantification of salivary copper
title_sort Study and optimization of a microfluidic paper based analytical device for the quantification of salivary copper
author Almeida, Adriana Raquel Gomes Tavares
author_facet Almeida, Adriana Raquel Gomes Tavares
author_role author
dc.contributor.none.fl_str_mv Mesquita, Raquel Beatriz Ribeiro de
Rangel, António Osmaro Santos Silva
Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Almeida, Adriana Raquel Gomes Tavares
dc.subject.por.fl_str_mv Copper
µPAD
Saliva
Colorimetric reaction
Cobre
Reação colorimétrica
Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Médica
topic Copper
µPAD
Saliva
Colorimetric reaction
Cobre
Reação colorimétrica
Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Médica
description Early diagnosis of pathology can promote a better recovery and treatment. Thus, the development the devices the rapid evaluation of the health condition, contribute to this process. In this context, in the recent years there has been growth in the development of low cost, easy to use devices. In this work, a microfluidic paper-based analytical device (μPAD) was development for the determination of copper in human saliva, being a form of rapid test. The device is suitable for the detection of excess copper, as consequently to change the healthy status. The development of the device involved several studies in order to get the rapid determination, accurate, cheaper and easy to use. Colorimetric reaction was chosen between copper and diphenylcarbazide create the complex of red colour. After the reaction time has elapsed, a scanner is used to obtain the digital image of μPAD detection zone, in order to calculate the absorbance based on the intensity of colour of each analytical local, obtained by Image J software. This device provides a copper determination in the range of 80.0 – 600 μg/L, a calibration curve of A= (0.0701±0.0008) [Cu2+] – (0.0043 ± 0.0004), the limit of detection of 17 μg/L and the limit of quantification of 56 μg/L. After inserting the sample in the μPAD, it can be digitized, within a time period of 20 - 40 min. The accuracy of the results was assessed by calculating recovery percentages using saliva from volunteers.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-22T01:30:30Z
2022-05-23
2022-04
2022-05-23T00:00:00Z
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