Analysis of the characteristics and cytotoxicity of titanium dioxide nanomaterials following simulated in vitro digestion
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/104326 |
Resumo: | Several metallic nanomaterials (NMs), such as titanium dioxide nanomaterials (TiO2), present beneficial properties with a broad range of innovative applications. The human population is exposed to TiO2, particularly by ingestion, due to its increasing use as a food additive and inclusion in dietary supplements and food packaging materials. Whether this oral exposure may lead to adverse local or systemic outcomes has been the subject of research, but studies have generated contradictory results, reflecting differences in the physicochemical properties of theTiO2 studied, effects of the surrounding matrix, and modifications during digestion. This work aimed to investigate the toxic effects of three different TiO2 NMs (NM-103, NM-103 and NM-105) on the gastrointestinal tract cells, Caco-2 and HT29-MTX-E12, after the use of the standardized static INFOGEST 2.0 in vitro digestion method to mimic human digestion of TiO2, contributing to hazard assessment. The results show that, for one of the digested TiO2 NMs studied (NM-105), a more pronounced toxicity occurs after exposure of HT29-MTX-E12 intestinal cells, as compared to undigested NM, concomitantly with subtle changes in characteristics of the NM. Thus, theinclusion of the digestion simulation in the safety evaluation of ingested NMs through in vitro bioassays can better integrate the modifications that NMs suffer in the organism. It is expected that such an approach will reduce uncertainties in the hazard assessment of ingested NMs for human health. |
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Analysis of the characteristics and cytotoxicity of titanium dioxide nanomaterials following simulated in vitro digestionCytotoxicityIngested titanium dioxideNanomaterialsStandardized in vitro digestionChemical Engineering(all)Materials Science(all)SDG 3 - Good Health and Well-beingSeveral metallic nanomaterials (NMs), such as titanium dioxide nanomaterials (TiO2), present beneficial properties with a broad range of innovative applications. The human population is exposed to TiO2, particularly by ingestion, due to its increasing use as a food additive and inclusion in dietary supplements and food packaging materials. Whether this oral exposure may lead to adverse local or systemic outcomes has been the subject of research, but studies have generated contradictory results, reflecting differences in the physicochemical properties of theTiO2 studied, effects of the surrounding matrix, and modifications during digestion. This work aimed to investigate the toxic effects of three different TiO2 NMs (NM-103, NM-103 and NM-105) on the gastrointestinal tract cells, Caco-2 and HT29-MTX-E12, after the use of the standardized static INFOGEST 2.0 in vitro digestion method to mimic human digestion of TiO2, contributing to hazard assessment. The results show that, for one of the digested TiO2 NMs studied (NM-105), a more pronounced toxicity occurs after exposure of HT29-MTX-E12 intestinal cells, as compared to undigested NM, concomitantly with subtle changes in characteristics of the NM. Thus, theinclusion of the digestion simulation in the safety evaluation of ingested NMs through in vitro bioassays can better integrate the modifications that NMs suffer in the organism. It is expected that such an approach will reduce uncertainties in the hazard assessment of ingested NMs for human health.Centro de Investigação em Saúde Pública (CISP/PHRC)Escola Nacional de Saúde Pública (ENSP)Centre for Toxicogenomics and Human Health (ToxOmics)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNBettencourt, AnaGonçalves, Lídia M.Gramacho, Ana C.Vieira, AdrianaRolo, DoraMartins, CarlaAssunção, RicardoAlvito, PaulaSilva, Maria JoãoLouro, Henriqueta2020-09-18T22:49:39Z2020-082020-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article18application/pdfhttp://hdl.handle.net/10362/104326eng2079-4991PURE: 19895522https://doi.org/10.3390/nano10081516info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:49:53Zoai:run.unl.pt:10362/104326Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:40:12.444241Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Analysis of the characteristics and cytotoxicity of titanium dioxide nanomaterials following simulated in vitro digestion |
title |
Analysis of the characteristics and cytotoxicity of titanium dioxide nanomaterials following simulated in vitro digestion |
spellingShingle |
Analysis of the characteristics and cytotoxicity of titanium dioxide nanomaterials following simulated in vitro digestion Bettencourt, Ana Cytotoxicity Ingested titanium dioxide Nanomaterials Standardized in vitro digestion Chemical Engineering(all) Materials Science(all) SDG 3 - Good Health and Well-being |
title_short |
Analysis of the characteristics and cytotoxicity of titanium dioxide nanomaterials following simulated in vitro digestion |
title_full |
Analysis of the characteristics and cytotoxicity of titanium dioxide nanomaterials following simulated in vitro digestion |
title_fullStr |
Analysis of the characteristics and cytotoxicity of titanium dioxide nanomaterials following simulated in vitro digestion |
title_full_unstemmed |
Analysis of the characteristics and cytotoxicity of titanium dioxide nanomaterials following simulated in vitro digestion |
title_sort |
Analysis of the characteristics and cytotoxicity of titanium dioxide nanomaterials following simulated in vitro digestion |
author |
Bettencourt, Ana |
author_facet |
Bettencourt, Ana Gonçalves, Lídia M. Gramacho, Ana C. Vieira, Adriana Rolo, Dora Martins, Carla Assunção, Ricardo Alvito, Paula Silva, Maria João Louro, Henriqueta |
author_role |
author |
author2 |
Gonçalves, Lídia M. Gramacho, Ana C. Vieira, Adriana Rolo, Dora Martins, Carla Assunção, Ricardo Alvito, Paula Silva, Maria João Louro, Henriqueta |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Centro de Investigação em Saúde Pública (CISP/PHRC) Escola Nacional de Saúde Pública (ENSP) Centre for Toxicogenomics and Human Health (ToxOmics) NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) RUN |
dc.contributor.author.fl_str_mv |
Bettencourt, Ana Gonçalves, Lídia M. Gramacho, Ana C. Vieira, Adriana Rolo, Dora Martins, Carla Assunção, Ricardo Alvito, Paula Silva, Maria João Louro, Henriqueta |
dc.subject.por.fl_str_mv |
Cytotoxicity Ingested titanium dioxide Nanomaterials Standardized in vitro digestion Chemical Engineering(all) Materials Science(all) SDG 3 - Good Health and Well-being |
topic |
Cytotoxicity Ingested titanium dioxide Nanomaterials Standardized in vitro digestion Chemical Engineering(all) Materials Science(all) SDG 3 - Good Health and Well-being |
description |
Several metallic nanomaterials (NMs), such as titanium dioxide nanomaterials (TiO2), present beneficial properties with a broad range of innovative applications. The human population is exposed to TiO2, particularly by ingestion, due to its increasing use as a food additive and inclusion in dietary supplements and food packaging materials. Whether this oral exposure may lead to adverse local or systemic outcomes has been the subject of research, but studies have generated contradictory results, reflecting differences in the physicochemical properties of theTiO2 studied, effects of the surrounding matrix, and modifications during digestion. This work aimed to investigate the toxic effects of three different TiO2 NMs (NM-103, NM-103 and NM-105) on the gastrointestinal tract cells, Caco-2 and HT29-MTX-E12, after the use of the standardized static INFOGEST 2.0 in vitro digestion method to mimic human digestion of TiO2, contributing to hazard assessment. The results show that, for one of the digested TiO2 NMs studied (NM-105), a more pronounced toxicity occurs after exposure of HT29-MTX-E12 intestinal cells, as compared to undigested NM, concomitantly with subtle changes in characteristics of the NM. Thus, theinclusion of the digestion simulation in the safety evaluation of ingested NMs through in vitro bioassays can better integrate the modifications that NMs suffer in the organism. It is expected that such an approach will reduce uncertainties in the hazard assessment of ingested NMs for human health. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-18T22:49:39Z 2020-08 2020-08-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/104326 |
url |
http://hdl.handle.net/10362/104326 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2079-4991 PURE: 19895522 https://doi.org/10.3390/nano10081516 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
18 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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