HPLC-DAD fingerprints combined with multivariate analysis of Epimedii Folium from major producing areas in Eastern Asia: effect of geographical origin and species
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/75215 |
Resumo: | The growth location and plant variety may influence the active components and biological activities of plants used in phytomedicine. In this study, nine sets of different Epimedii Folium, from different representative cultivation locations and Epimedium species, were collected for comparison, using HPLC-DAD combined with multivariate analysis. The objective was to investigate the influence of geographical origin and Epimedium species on the quality of Epimedii Folium, and provide applicable guidance for cultivation and quality control of Epimedii Folium. Several Epimedium spp. sets were used to establish the HPLC-DAD fingerprints and 91 peaks (compounds) were selected for the multivariate analysis. Major compounds were analyzed by HPLC-DAD combined with principal component analysis (PCA). HPLC quantitative analysis of known bioactive compounds was performed. Application of PCA to HPLC data showed that Epimedium samples sharing the same geographical origin or species clustered together, indicating that both species and geographical origin have impacts on the quality of Epimedii Folium. The major bioactive flavonoid compounds, epimedin C, icariin and baohuoside I, were identified and quantified. The concentration of bioactive compounds was significantly influenced both by species and geographical origin. E. sagittatum from Sichuan showed the highest content of bioactive compounds. The results showed that both Epimedium species and geographical origin have strong impact into quality of Epimedii Folium. HPLC data combined with multivariate analysis is a suitable approach to inform the selection of cultivation areas and choose Epimedium spp. most suitable for different geographical areas, resulting in improved quality of Epimedii Folium. |
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HPLC-DAD fingerprints combined with multivariate analysis of Epimedii Folium from major producing areas in Eastern Asia: effect of geographical origin and speciesEpimedium sphigh performance liquid chromatography (HPLC)principal component analysis (PCA)epimedin Cicariinbaohuoside ICiências Naturais::Ciências BiológicasScience & TechnologyThe growth location and plant variety may influence the active components and biological activities of plants used in phytomedicine. In this study, nine sets of different Epimedii Folium, from different representative cultivation locations and Epimedium species, were collected for comparison, using HPLC-DAD combined with multivariate analysis. The objective was to investigate the influence of geographical origin and Epimedium species on the quality of Epimedii Folium, and provide applicable guidance for cultivation and quality control of Epimedii Folium. Several Epimedium spp. sets were used to establish the HPLC-DAD fingerprints and 91 peaks (compounds) were selected for the multivariate analysis. Major compounds were analyzed by HPLC-DAD combined with principal component analysis (PCA). HPLC quantitative analysis of known bioactive compounds was performed. Application of PCA to HPLC data showed that Epimedium samples sharing the same geographical origin or species clustered together, indicating that both species and geographical origin have impacts on the quality of Epimedii Folium. The major bioactive flavonoid compounds, epimedin C, icariin and baohuoside I, were identified and quantified. The concentration of bioactive compounds was significantly influenced both by species and geographical origin. E. sagittatum from Sichuan showed the highest content of bioactive compounds. The results showed that both Epimedium species and geographical origin have strong impact into quality of Epimedii Folium. HPLC data combined with multivariate analysis is a suitable approach to inform the selection of cultivation areas and choose Epimedium spp. most suitable for different geographical areas, resulting in improved quality of Epimedii Folium.This work was supported by Incubation Project on State Key Laboratory of Biological Resources and Ecological Environment of Qinba Areas (SLGPT2019KF04-04), China, and the ERDF through the COMPETE2020—Programa Operacional Competitividade e Internacionalização (POCI), Portugal.info:eu-repo/semantics/publishedVersionFrontiers Media S.A.Universidade do MinhoLi, BenLima, Marta R. M.Nie, YuhaoXu, LongLiu, XiangYuan, HongchaoChen, ChenDias, AlbertoZhang, Xiaoying2021-11-262021-11-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/75215engLi, Ben; Lima, Marta R. M.; Nie, Yuhao; Xu, Long; Liu, Xiang; Yuan, Hongchao; Chen, Chen; Dias, Alberto; Zhang, Xiaoying, HPLC-DAD Fingerprints Combined With Multivariate Analysis of Epimedii Folium From Major Producing Areas in Eastern Asia: Effect of Geographical Origin and Species. Frontiers in Pharmacology, 12(761551), 20211663-981210.3389/fphar.2021.761551761551https://www.frontiersin.org/articles/10.3389/fphar.2021.761551/fullinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:11:06Zoai:repositorium.sdum.uminho.pt:1822/75215Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:02:48.815932Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
HPLC-DAD fingerprints combined with multivariate analysis of Epimedii Folium from major producing areas in Eastern Asia: effect of geographical origin and species |
title |
HPLC-DAD fingerprints combined with multivariate analysis of Epimedii Folium from major producing areas in Eastern Asia: effect of geographical origin and species |
spellingShingle |
HPLC-DAD fingerprints combined with multivariate analysis of Epimedii Folium from major producing areas in Eastern Asia: effect of geographical origin and species Li, Ben Epimedium sp high performance liquid chromatography (HPLC) principal component analysis (PCA) epimedin C icariin baohuoside I Ciências Naturais::Ciências Biológicas Science & Technology |
title_short |
HPLC-DAD fingerprints combined with multivariate analysis of Epimedii Folium from major producing areas in Eastern Asia: effect of geographical origin and species |
title_full |
HPLC-DAD fingerprints combined with multivariate analysis of Epimedii Folium from major producing areas in Eastern Asia: effect of geographical origin and species |
title_fullStr |
HPLC-DAD fingerprints combined with multivariate analysis of Epimedii Folium from major producing areas in Eastern Asia: effect of geographical origin and species |
title_full_unstemmed |
HPLC-DAD fingerprints combined with multivariate analysis of Epimedii Folium from major producing areas in Eastern Asia: effect of geographical origin and species |
title_sort |
HPLC-DAD fingerprints combined with multivariate analysis of Epimedii Folium from major producing areas in Eastern Asia: effect of geographical origin and species |
author |
Li, Ben |
author_facet |
Li, Ben Lima, Marta R. M. Nie, Yuhao Xu, Long Liu, Xiang Yuan, Hongchao Chen, Chen Dias, Alberto Zhang, Xiaoying |
author_role |
author |
author2 |
Lima, Marta R. M. Nie, Yuhao Xu, Long Liu, Xiang Yuan, Hongchao Chen, Chen Dias, Alberto Zhang, Xiaoying |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Li, Ben Lima, Marta R. M. Nie, Yuhao Xu, Long Liu, Xiang Yuan, Hongchao Chen, Chen Dias, Alberto Zhang, Xiaoying |
dc.subject.por.fl_str_mv |
Epimedium sp high performance liquid chromatography (HPLC) principal component analysis (PCA) epimedin C icariin baohuoside I Ciências Naturais::Ciências Biológicas Science & Technology |
topic |
Epimedium sp high performance liquid chromatography (HPLC) principal component analysis (PCA) epimedin C icariin baohuoside I Ciências Naturais::Ciências Biológicas Science & Technology |
description |
The growth location and plant variety may influence the active components and biological activities of plants used in phytomedicine. In this study, nine sets of different Epimedii Folium, from different representative cultivation locations and Epimedium species, were collected for comparison, using HPLC-DAD combined with multivariate analysis. The objective was to investigate the influence of geographical origin and Epimedium species on the quality of Epimedii Folium, and provide applicable guidance for cultivation and quality control of Epimedii Folium. Several Epimedium spp. sets were used to establish the HPLC-DAD fingerprints and 91 peaks (compounds) were selected for the multivariate analysis. Major compounds were analyzed by HPLC-DAD combined with principal component analysis (PCA). HPLC quantitative analysis of known bioactive compounds was performed. Application of PCA to HPLC data showed that Epimedium samples sharing the same geographical origin or species clustered together, indicating that both species and geographical origin have impacts on the quality of Epimedii Folium. The major bioactive flavonoid compounds, epimedin C, icariin and baohuoside I, were identified and quantified. The concentration of bioactive compounds was significantly influenced both by species and geographical origin. E. sagittatum from Sichuan showed the highest content of bioactive compounds. The results showed that both Epimedium species and geographical origin have strong impact into quality of Epimedii Folium. HPLC data combined with multivariate analysis is a suitable approach to inform the selection of cultivation areas and choose Epimedium spp. most suitable for different geographical areas, resulting in improved quality of Epimedii Folium. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-26 2021-11-26T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/75215 |
url |
http://hdl.handle.net/1822/75215 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Li, Ben; Lima, Marta R. M.; Nie, Yuhao; Xu, Long; Liu, Xiang; Yuan, Hongchao; Chen, Chen; Dias, Alberto; Zhang, Xiaoying, HPLC-DAD Fingerprints Combined With Multivariate Analysis of Epimedii Folium From Major Producing Areas in Eastern Asia: Effect of Geographical Origin and Species. Frontiers in Pharmacology, 12(761551), 2021 1663-9812 10.3389/fphar.2021.761551 761551 https://www.frontiersin.org/articles/10.3389/fphar.2021.761551/full |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media S.A. |
publisher.none.fl_str_mv |
Frontiers Media S.A. |
dc.source.none.fl_str_mv |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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