Subcritical water extraction and hydrolysis of cod (Gadus morhua) frames to produce bioactive protein extracts

Detalhes bibliográficos
Autor(a) principal: Melgosa, Rodrigo
Data de Publicação: 2021
Outros Autores: Marques, Marta, Paiva, Alexandre, Bernardo, Ana, Fernández, Naiara, Sá-Nogueira, Isabel, Simões, Pedro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/133130
Resumo: UIDB/QUI/50006/2020 UIDP/04378/2020 UIDB/04378/2020 PTDC/ASP-PES/28399/2017 UIDB/04462/2020 UIDP/04462/2020 IF/01146/2015
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spelling Subcritical water extraction and hydrolysis of cod (Gadus morhua) frames to produce bioactive protein extractsAntioxidant activityAntiprolifera-tive activityCod framesFish protein hydrolysateSubcritical waterFood ScienceMicrobiologyHealth(social science)Health Professions (miscellaneous)Plant ScienceUIDB/QUI/50006/2020 UIDP/04378/2020 UIDB/04378/2020 PTDC/ASP-PES/28399/2017 UIDB/04462/2020 UIDP/04462/2020 IF/01146/2015The valorization of Atlantic cod (Gadus morhua) frames from a filleting industry was investigated using subcritical water extraction and hydrolysis (SBW) at different temperatures (90, 140, 190 and 250◦C) and 100 bar to obtain extracts rich in proteins, peptides and amino acids. Up to 57.7 g of extract per 100 g of codfish frames were obtained, with nearly total recovery of the protein fraction. At each temperature, protein extracts of decreasing molecular weight were obtained, according to SEC-GPC results. Most of the protein present in the raw material and extracts was collagen and collagen fragments, as suggested by the amino acid profile. Codfish SBW extracts did not show cytotoxicity in the range of concentrations tested and the protein extract obtained at the lowest temperature (90◦C) showed the highest anti-inflammatory potential in human intestinal epithelium cell model. The mineralized residue left after SBW treatment of cod frames was identified as practically pure, crystalline, hydroxyapatite, that may find applications in biomedical field and hard-tissue engineering. This study shows the possible valorization of cod frames using green extraction methods such as SBW process to obtain protein extracts for food and nutraceutical applications.LAQV@REQUIMTEDQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNMelgosa, RodrigoMarques, MartaPaiva, AlexandreBernardo, AnaFernández, NaiaraSá-Nogueira, IsabelSimões, Pedro2022-02-17T23:19:39Z2021-05-282021-05-28T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/133130eng2304-8158PURE: 36692166https://doi.org/10.3390/foods10061222info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:11:49Zoai:run.unl.pt:10362/133130Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:47:42.784502Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Subcritical water extraction and hydrolysis of cod (Gadus morhua) frames to produce bioactive protein extracts
title Subcritical water extraction and hydrolysis of cod (Gadus morhua) frames to produce bioactive protein extracts
spellingShingle Subcritical water extraction and hydrolysis of cod (Gadus morhua) frames to produce bioactive protein extracts
Melgosa, Rodrigo
Antioxidant activity
Antiprolifera-tive activity
Cod frames
Fish protein hydrolysate
Subcritical water
Food Science
Microbiology
Health(social science)
Health Professions (miscellaneous)
Plant Science
title_short Subcritical water extraction and hydrolysis of cod (Gadus morhua) frames to produce bioactive protein extracts
title_full Subcritical water extraction and hydrolysis of cod (Gadus morhua) frames to produce bioactive protein extracts
title_fullStr Subcritical water extraction and hydrolysis of cod (Gadus morhua) frames to produce bioactive protein extracts
title_full_unstemmed Subcritical water extraction and hydrolysis of cod (Gadus morhua) frames to produce bioactive protein extracts
title_sort Subcritical water extraction and hydrolysis of cod (Gadus morhua) frames to produce bioactive protein extracts
author Melgosa, Rodrigo
author_facet Melgosa, Rodrigo
Marques, Marta
Paiva, Alexandre
Bernardo, Ana
Fernández, Naiara
Sá-Nogueira, Isabel
Simões, Pedro
author_role author
author2 Marques, Marta
Paiva, Alexandre
Bernardo, Ana
Fernández, Naiara
Sá-Nogueira, Isabel
Simões, Pedro
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Melgosa, Rodrigo
Marques, Marta
Paiva, Alexandre
Bernardo, Ana
Fernández, Naiara
Sá-Nogueira, Isabel
Simões, Pedro
dc.subject.por.fl_str_mv Antioxidant activity
Antiprolifera-tive activity
Cod frames
Fish protein hydrolysate
Subcritical water
Food Science
Microbiology
Health(social science)
Health Professions (miscellaneous)
Plant Science
topic Antioxidant activity
Antiprolifera-tive activity
Cod frames
Fish protein hydrolysate
Subcritical water
Food Science
Microbiology
Health(social science)
Health Professions (miscellaneous)
Plant Science
description UIDB/QUI/50006/2020 UIDP/04378/2020 UIDB/04378/2020 PTDC/ASP-PES/28399/2017 UIDB/04462/2020 UIDP/04462/2020 IF/01146/2015
publishDate 2021
dc.date.none.fl_str_mv 2021-05-28
2021-05-28T00:00:00Z
2022-02-17T23:19:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/133130
url http://hdl.handle.net/10362/133130
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2304-8158
PURE: 36692166
https://doi.org/10.3390/foods10061222
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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