High-fat diet results in postprandial insulin resistance that involves parasympathetic dysfunction
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2010 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | und |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/141771 |
Resumo: | We are indebted to Dr Zhi Ming for sharing technical expertise and Gerald Nolette from the Central Animal Care Services of the University of Manitoba for animal care assistance. The present work was supported by the Portuguese Foundation for Science and Technology (FCT, grant POCI/ SAU-OBS/56 716/2004), the Portuguese Society of Endocrinology, Diabetes and Metabolism and the Canadian Diabetes Association and Canadian Institutes of Health Research (operating grants). M. P. M. and W. W. L. were involved in the conceptual work; R. A. A. was involved in all aspects of the work presented; D. J. L. and J. S. conducted insulin and GSH quantifications; A. G. O. was involved in data analysis. There are no conflicts of interests to report. |
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High-fat diet results in postprandial insulin resistance that involves parasympathetic dysfunctionParasympatheticnitric-oxidevisceraladipositypostmealadipose-tissueobesenervesskeletal-musclewhiteblockadeoxidativeHigh-fatdietzuckerObesitystressglucoserattestInsulinristresistancenervous-systemsynthasesensitivityObesityHigh-fat dietInsulin resistanceParasympathetic nervesSDG 3 - Good Health and Well-beingWe are indebted to Dr Zhi Ming for sharing technical expertise and Gerald Nolette from the Central Animal Care Services of the University of Manitoba for animal care assistance. The present work was supported by the Portuguese Foundation for Science and Technology (FCT, grant POCI/ SAU-OBS/56 716/2004), the Portuguese Society of Endocrinology, Diabetes and Metabolism and the Canadian Diabetes Association and Canadian Institutes of Health Research (operating grants). M. P. M. and W. W. L. were involved in the conceptual work; R. A. A. was involved in all aspects of the work presented; D. J. L. and J. S. conducted insulin and GSH quantifications; A. G. O. was involved in data analysis. There are no conflicts of interests to report.Different diets have distinct impacts on glucose homoeostasis, for which insulin sensitivity (IS) after a meal (postprandial IS) is highly relevant. Postprandial IS depends upon hepatic parasympathetic activation and glutathione content elevation. We tested the hypothesis that postprandial IS is compromised in high-fat diet (HFD)-induced obesity. Sprague-Dawley rats were fed a standard diet (STD, n 10), 1-week HFD (n 9) or 4-week HFD (n 8). IS was tested in postprandial state using the rapid IS test (RIST) before and after the blockade of the parasympathetic nerves (atropine, 1 mg/kg); parasympathetic-dependent IS was obtained from the difference between control and post-atropine RIST. Fasting IS was also assessed in the STD-fed rats (n 4) and 4-week HFD-fed rats (n 3) using the RIST. Whole-body fat and regional fat pads were heavier in the 1-week HFD-fed rats (79.8 (SE 7.9) and 23.7 (SE 1.0) g, respectively) or 4-week HFD-fed rats (106.5 (SE 6.1) and 30.1 (SE 1.4) g, respectively) than in the STD-fed rats (32.5 (SE 3.7) and 13.7 (SE 1.0) g, respectively; P < 0.001). Fasted-state IS was similar between the groups studied. Postprandial IS was higher in the STD-fed rats (185.8 (SE 5.6) mg glucose/kg body weight (bw)) than in both the 1-week HFD-fed rats (108.8 (SE 2.9) mg glucose/kg bw; P < 0.001) and 4-week HFD-fed rats (69.3 (SE 2.6) mg glucose/kg bw; P < 0.001). Parasympathetic-dependent IS was impaired in both HFD-fed groups (STD, 108.9 (SE 3.9) mg glucose/kg bw; 1-week HFD, 38.6 (SE 4.2) mg glucose/kg bw; 4-week HFD, 5.4 (SE 1.7) mg glucose/kg bw; P < 0.001). Total (postprandial) and parasympathetic-dependent IS correlated negatively with whole-body fat (R-2 0.81 and 0.87) and regional adiposity (R-2 0.85 and 0.79). In conclusion, fat accumulation induced by HFD is associated with postprandial insulin resistance, but not with fasting insulin resistance. HFD-associated postprandial insulin resistance is largely mediated by impairment of parasympathetic-dependent insulin action, which correlates with adiposity. British Journal of NutritionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNAfonso, Ricardo ALautt, W. WayneSchafer, JoshuaLegare, Dallas J.Oliveira, António GMacedo, M Paula2024-01-24T01:31:44Z2010-01-012010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/141771und0007-1145PURE: 312503https://doi.org/10.1017/S0007114510002400info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:19:02Zoai:run.unl.pt:10362/141771Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:50:03.669416Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
High-fat diet results in postprandial insulin resistance that involves parasympathetic dysfunction |
| title |
High-fat diet results in postprandial insulin resistance that involves parasympathetic dysfunction |
| spellingShingle |
High-fat diet results in postprandial insulin resistance that involves parasympathetic dysfunction Afonso, Ricardo A Parasympathetic nitric-oxide visceral adiposity postmeal adipose-tissue obese nerves skeletal-muscle white blockade oxidative High-fat diet zucker Obesity stress glucose rat test Insulin rist resistance nervous-system synthase sensitivity Obesity High-fat diet Insulin resistance Parasympathetic nerves SDG 3 - Good Health and Well-being |
| title_short |
High-fat diet results in postprandial insulin resistance that involves parasympathetic dysfunction |
| title_full |
High-fat diet results in postprandial insulin resistance that involves parasympathetic dysfunction |
| title_fullStr |
High-fat diet results in postprandial insulin resistance that involves parasympathetic dysfunction |
| title_full_unstemmed |
High-fat diet results in postprandial insulin resistance that involves parasympathetic dysfunction |
| title_sort |
High-fat diet results in postprandial insulin resistance that involves parasympathetic dysfunction |
| author |
Afonso, Ricardo A |
| author_facet |
Afonso, Ricardo A Lautt, W. Wayne Schafer, Joshua Legare, Dallas J. Oliveira, António G Macedo, M Paula |
| author_role |
author |
| author2 |
Lautt, W. Wayne Schafer, Joshua Legare, Dallas J. Oliveira, António G Macedo, M Paula |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) RUN |
| dc.contributor.author.fl_str_mv |
Afonso, Ricardo A Lautt, W. Wayne Schafer, Joshua Legare, Dallas J. Oliveira, António G Macedo, M Paula |
| dc.subject.por.fl_str_mv |
Parasympathetic nitric-oxide visceral adiposity postmeal adipose-tissue obese nerves skeletal-muscle white blockade oxidative High-fat diet zucker Obesity stress glucose rat test Insulin rist resistance nervous-system synthase sensitivity Obesity High-fat diet Insulin resistance Parasympathetic nerves SDG 3 - Good Health and Well-being |
| topic |
Parasympathetic nitric-oxide visceral adiposity postmeal adipose-tissue obese nerves skeletal-muscle white blockade oxidative High-fat diet zucker Obesity stress glucose rat test Insulin rist resistance nervous-system synthase sensitivity Obesity High-fat diet Insulin resistance Parasympathetic nerves SDG 3 - Good Health and Well-being |
| description |
We are indebted to Dr Zhi Ming for sharing technical expertise and Gerald Nolette from the Central Animal Care Services of the University of Manitoba for animal care assistance. The present work was supported by the Portuguese Foundation for Science and Technology (FCT, grant POCI/ SAU-OBS/56 716/2004), the Portuguese Society of Endocrinology, Diabetes and Metabolism and the Canadian Diabetes Association and Canadian Institutes of Health Research (operating grants). M. P. M. and W. W. L. were involved in the conceptual work; R. A. A. was involved in all aspects of the work presented; D. J. L. and J. S. conducted insulin and GSH quantifications; A. G. O. was involved in data analysis. There are no conflicts of interests to report. |
| publishDate |
2010 |
| dc.date.none.fl_str_mv |
2010-01-01 2010-01-01T00:00:00Z 2024-01-24T01:31:44Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/141771 |
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http://hdl.handle.net/10362/141771 |
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und |
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und |
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0007-1145 PURE: 312503 https://doi.org/10.1017/S0007114510002400 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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