The Crystal Structure of the Escherichia coli Autoinducer-2 Processing Protein LsrF

Detalhes bibliográficos
Autor(a) principal: Diaz, Z.
Data de Publicação: 2009
Outros Autores: Xavier, K. B., Miller, S. T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/5860
Resumo: PLOS ONE, 4(8):ARTe6820
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spelling The Crystal Structure of the Escherichia coli Autoinducer-2 Processing Protein LsrFPLOS ONE, 4(8):ARTe6820Many bacteria produce and respond to the quorum sensing signal autoinducer-2 (AI-2). Escherichia coli and Salmonella typhimurium are among the species with the lsr operon, an operon containing AI-2 transport and processing genes that are up regulated in response to AI-2. One of the Lsr proteins, LsrF, has been implicated in processing the phosphorylated form of AI-2. Here, we present the structure of LsrF, unliganded and in complex with two phospho-AI-2 analogues, ribose-5-phosphate and ribulose-5-phosphate. The crystal structure shows that LsrF is a decamer of (alpha beta)(8)-barrels that exhibit a previously unseen N- terminal domain swap and have high structural homology with aldolases that process phosphorylated sugars. Ligand binding sites and key catalytic residues are structurally conserved, strongly implicating LsrF as a class I aldolase.Public Library of ScienceRUNDiaz, Z.Xavier, K. B.Miller, S. T.2011-06-27T14:27:16Z2009-01-01T00:00:00Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/5860eng1932-6203info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-10T15:25:42ZPortal AgregadorONG
dc.title.none.fl_str_mv The Crystal Structure of the Escherichia coli Autoinducer-2 Processing Protein LsrF
title The Crystal Structure of the Escherichia coli Autoinducer-2 Processing Protein LsrF
spellingShingle The Crystal Structure of the Escherichia coli Autoinducer-2 Processing Protein LsrF
Diaz, Z.
title_short The Crystal Structure of the Escherichia coli Autoinducer-2 Processing Protein LsrF
title_full The Crystal Structure of the Escherichia coli Autoinducer-2 Processing Protein LsrF
title_fullStr The Crystal Structure of the Escherichia coli Autoinducer-2 Processing Protein LsrF
title_full_unstemmed The Crystal Structure of the Escherichia coli Autoinducer-2 Processing Protein LsrF
title_sort The Crystal Structure of the Escherichia coli Autoinducer-2 Processing Protein LsrF
author Diaz, Z.
author_facet Diaz, Z.
Xavier, K. B.
Miller, S. T.
author_role author
author2 Xavier, K. B.
Miller, S. T.
author2_role author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Diaz, Z.
Xavier, K. B.
Miller, S. T.
description PLOS ONE, 4(8):ARTe6820
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01T00:00:00Z
2009-01-01
2011-06-27T14:27:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/5860
url http://hdl.handle.net/10362/5860
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1932-6203
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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