Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death

Detalhes bibliográficos
Autor(a) principal: Giannattasio, Sergio
Data de Publicação: 2005
Outros Autores: Guaragnella, Nicoletta, Côrte-Real, Manuela, Passarella, Salvatore, Marra, Ersilia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/5087
Resumo: In this work evidence is presented that acid stress adaptation protects Saccharomyces cerevisiae from acetic acid-mediated programmed cell death. Exponential-phase yeast cells, non-adapted or adapted to acid stress by 30 min incubation in rich medium set at pH 3.0 with HCl, have been exposed to increasing concentrations of acetic acid and time course changes of cell viability have been assessed. Adapted cells, in contrast to non-adapted cells, when exposed to 80 mM acetic acid for 200 min did not display loss of cell viability associated to morphological alterations typical of apoptosis. Thus, 80 mM acetic acid death-inducing conditions were selected to further characterize the early molecular events leading to such active cell death process. Catalase was specifically activated during acid stress adaptation and protection against acetic acid-induced death was associated with maintenance of its activity during treatment with 80 mM acetic acid. On the other hand, intracellular superoxide dismutase activity was found present at comparable levels both in adapted and in dying yeast cells, excepting in non-adapted cells which displayed a maximum activity value after 15 min acetic acid exposure, corresponding to more than 80% cell viability. This study gives first experimental evidence that H2O2, rather than superoxide, detoxification may have a major role in preventing yeast cell death in response to acetic acid. The results, as a whole, suggest that commitment of S. cerevisiae to a programmed cell death process in response to acetic acid is mediated through a ROS-dependent apoptotic pathway.
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spelling Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell deathSaccharomyces cerevisiaeProgrammed cell deathStrong acid stressSuperoxide dismutaseCatalaseScience & TechnologyIn this work evidence is presented that acid stress adaptation protects Saccharomyces cerevisiae from acetic acid-mediated programmed cell death. Exponential-phase yeast cells, non-adapted or adapted to acid stress by 30 min incubation in rich medium set at pH 3.0 with HCl, have been exposed to increasing concentrations of acetic acid and time course changes of cell viability have been assessed. Adapted cells, in contrast to non-adapted cells, when exposed to 80 mM acetic acid for 200 min did not display loss of cell viability associated to morphological alterations typical of apoptosis. Thus, 80 mM acetic acid death-inducing conditions were selected to further characterize the early molecular events leading to such active cell death process. Catalase was specifically activated during acid stress adaptation and protection against acetic acid-induced death was associated with maintenance of its activity during treatment with 80 mM acetic acid. On the other hand, intracellular superoxide dismutase activity was found present at comparable levels both in adapted and in dying yeast cells, excepting in non-adapted cells which displayed a maximum activity value after 15 min acetic acid exposure, corresponding to more than 80% cell viability. This study gives first experimental evidence that H2O2, rather than superoxide, detoxification may have a major role in preventing yeast cell death in response to acetic acid. The results, as a whole, suggest that commitment of S. cerevisiae to a programmed cell death process in response to acetic acid is mediated through a ROS-dependent apoptotic pathway.ElsevierUniversidade do MinhoGiannattasio, SergioGuaragnella, NicolettaCôrte-Real, ManuelaPassarella, SalvatoreMarra, Ersilia20052005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/5087eng"Gene". ISSN 0378-1119. 354 (2005) 93-98.0378-111910.1016/j.gene.2005.03.03015894436www.sciencedirect.com/science/journal/03781119info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:22:40Zoai:repositorium.sdum.uminho.pt:1822/5087Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:16:11.394764Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death
title Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death
spellingShingle Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death
Giannattasio, Sergio
Saccharomyces cerevisiae
Programmed cell death
Strong acid stress
Superoxide dismutase
Catalase
Science & Technology
title_short Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death
title_full Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death
title_fullStr Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death
title_full_unstemmed Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death
title_sort Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death
author Giannattasio, Sergio
author_facet Giannattasio, Sergio
Guaragnella, Nicoletta
Côrte-Real, Manuela
Passarella, Salvatore
Marra, Ersilia
author_role author
author2 Guaragnella, Nicoletta
Côrte-Real, Manuela
Passarella, Salvatore
Marra, Ersilia
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Giannattasio, Sergio
Guaragnella, Nicoletta
Côrte-Real, Manuela
Passarella, Salvatore
Marra, Ersilia
dc.subject.por.fl_str_mv Saccharomyces cerevisiae
Programmed cell death
Strong acid stress
Superoxide dismutase
Catalase
Science & Technology
topic Saccharomyces cerevisiae
Programmed cell death
Strong acid stress
Superoxide dismutase
Catalase
Science & Technology
description In this work evidence is presented that acid stress adaptation protects Saccharomyces cerevisiae from acetic acid-mediated programmed cell death. Exponential-phase yeast cells, non-adapted or adapted to acid stress by 30 min incubation in rich medium set at pH 3.0 with HCl, have been exposed to increasing concentrations of acetic acid and time course changes of cell viability have been assessed. Adapted cells, in contrast to non-adapted cells, when exposed to 80 mM acetic acid for 200 min did not display loss of cell viability associated to morphological alterations typical of apoptosis. Thus, 80 mM acetic acid death-inducing conditions were selected to further characterize the early molecular events leading to such active cell death process. Catalase was specifically activated during acid stress adaptation and protection against acetic acid-induced death was associated with maintenance of its activity during treatment with 80 mM acetic acid. On the other hand, intracellular superoxide dismutase activity was found present at comparable levels both in adapted and in dying yeast cells, excepting in non-adapted cells which displayed a maximum activity value after 15 min acetic acid exposure, corresponding to more than 80% cell viability. This study gives first experimental evidence that H2O2, rather than superoxide, detoxification may have a major role in preventing yeast cell death in response to acetic acid. The results, as a whole, suggest that commitment of S. cerevisiae to a programmed cell death process in response to acetic acid is mediated through a ROS-dependent apoptotic pathway.
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/5087
url http://hdl.handle.net/1822/5087
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Gene". ISSN 0378-1119. 354 (2005) 93-98.
0378-1119
10.1016/j.gene.2005.03.030
15894436
www.sciencedirect.com/science/journal/03781119
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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