Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/5087 |
Resumo: | In this work evidence is presented that acid stress adaptation protects Saccharomyces cerevisiae from acetic acid-mediated programmed cell death. Exponential-phase yeast cells, non-adapted or adapted to acid stress by 30 min incubation in rich medium set at pH 3.0 with HCl, have been exposed to increasing concentrations of acetic acid and time course changes of cell viability have been assessed. Adapted cells, in contrast to non-adapted cells, when exposed to 80 mM acetic acid for 200 min did not display loss of cell viability associated to morphological alterations typical of apoptosis. Thus, 80 mM acetic acid death-inducing conditions were selected to further characterize the early molecular events leading to such active cell death process. Catalase was specifically activated during acid stress adaptation and protection against acetic acid-induced death was associated with maintenance of its activity during treatment with 80 mM acetic acid. On the other hand, intracellular superoxide dismutase activity was found present at comparable levels both in adapted and in dying yeast cells, excepting in non-adapted cells which displayed a maximum activity value after 15 min acetic acid exposure, corresponding to more than 80% cell viability. This study gives first experimental evidence that H2O2, rather than superoxide, detoxification may have a major role in preventing yeast cell death in response to acetic acid. The results, as a whole, suggest that commitment of S. cerevisiae to a programmed cell death process in response to acetic acid is mediated through a ROS-dependent apoptotic pathway. |
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Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell deathSaccharomyces cerevisiaeProgrammed cell deathStrong acid stressSuperoxide dismutaseCatalaseScience & TechnologyIn this work evidence is presented that acid stress adaptation protects Saccharomyces cerevisiae from acetic acid-mediated programmed cell death. Exponential-phase yeast cells, non-adapted or adapted to acid stress by 30 min incubation in rich medium set at pH 3.0 with HCl, have been exposed to increasing concentrations of acetic acid and time course changes of cell viability have been assessed. Adapted cells, in contrast to non-adapted cells, when exposed to 80 mM acetic acid for 200 min did not display loss of cell viability associated to morphological alterations typical of apoptosis. Thus, 80 mM acetic acid death-inducing conditions were selected to further characterize the early molecular events leading to such active cell death process. Catalase was specifically activated during acid stress adaptation and protection against acetic acid-induced death was associated with maintenance of its activity during treatment with 80 mM acetic acid. On the other hand, intracellular superoxide dismutase activity was found present at comparable levels both in adapted and in dying yeast cells, excepting in non-adapted cells which displayed a maximum activity value after 15 min acetic acid exposure, corresponding to more than 80% cell viability. This study gives first experimental evidence that H2O2, rather than superoxide, detoxification may have a major role in preventing yeast cell death in response to acetic acid. The results, as a whole, suggest that commitment of S. cerevisiae to a programmed cell death process in response to acetic acid is mediated through a ROS-dependent apoptotic pathway.ElsevierUniversidade do MinhoGiannattasio, SergioGuaragnella, NicolettaCôrte-Real, ManuelaPassarella, SalvatoreMarra, Ersilia20052005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/5087eng"Gene". ISSN 0378-1119. 354 (2005) 93-98.0378-111910.1016/j.gene.2005.03.03015894436www.sciencedirect.com/science/journal/03781119info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:22:40Zoai:repositorium.sdum.uminho.pt:1822/5087Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:16:11.394764Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death |
title |
Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death |
spellingShingle |
Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death Giannattasio, Sergio Saccharomyces cerevisiae Programmed cell death Strong acid stress Superoxide dismutase Catalase Science & Technology |
title_short |
Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death |
title_full |
Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death |
title_fullStr |
Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death |
title_full_unstemmed |
Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death |
title_sort |
Acid stress adaptation protects saccharomyces cerevisiae from acetic acid-induced programme cell death |
author |
Giannattasio, Sergio |
author_facet |
Giannattasio, Sergio Guaragnella, Nicoletta Côrte-Real, Manuela Passarella, Salvatore Marra, Ersilia |
author_role |
author |
author2 |
Guaragnella, Nicoletta Côrte-Real, Manuela Passarella, Salvatore Marra, Ersilia |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Giannattasio, Sergio Guaragnella, Nicoletta Côrte-Real, Manuela Passarella, Salvatore Marra, Ersilia |
dc.subject.por.fl_str_mv |
Saccharomyces cerevisiae Programmed cell death Strong acid stress Superoxide dismutase Catalase Science & Technology |
topic |
Saccharomyces cerevisiae Programmed cell death Strong acid stress Superoxide dismutase Catalase Science & Technology |
description |
In this work evidence is presented that acid stress adaptation protects Saccharomyces cerevisiae from acetic acid-mediated programmed cell death. Exponential-phase yeast cells, non-adapted or adapted to acid stress by 30 min incubation in rich medium set at pH 3.0 with HCl, have been exposed to increasing concentrations of acetic acid and time course changes of cell viability have been assessed. Adapted cells, in contrast to non-adapted cells, when exposed to 80 mM acetic acid for 200 min did not display loss of cell viability associated to morphological alterations typical of apoptosis. Thus, 80 mM acetic acid death-inducing conditions were selected to further characterize the early molecular events leading to such active cell death process. Catalase was specifically activated during acid stress adaptation and protection against acetic acid-induced death was associated with maintenance of its activity during treatment with 80 mM acetic acid. On the other hand, intracellular superoxide dismutase activity was found present at comparable levels both in adapted and in dying yeast cells, excepting in non-adapted cells which displayed a maximum activity value after 15 min acetic acid exposure, corresponding to more than 80% cell viability. This study gives first experimental evidence that H2O2, rather than superoxide, detoxification may have a major role in preventing yeast cell death in response to acetic acid. The results, as a whole, suggest that commitment of S. cerevisiae to a programmed cell death process in response to acetic acid is mediated through a ROS-dependent apoptotic pathway. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005 2005-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/5087 |
url |
http://hdl.handle.net/1822/5087 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Gene". ISSN 0378-1119. 354 (2005) 93-98. 0378-1119 10.1016/j.gene.2005.03.030 15894436 www.sciencedirect.com/science/journal/03781119 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132609923514368 |