DNA methylation changes in fibromyalgia suggest the role of the immune-inflammatory response and central sensitization
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/75921 |
Resumo: | Fibromyalgia (FM) has been explained as a result of gene-environment interactions. The present study aims to verify DNA methylation differences in eleven candidate genome regions previously associated to FM, evaluating DNA methylation patterns as potential disease biomarkers. DNA methylation was analyzed through bisulfite sequencing, comparing 42 FM women and their 42 healthy sisters. The associations between the level of methylation in these regions were further explored through a network analysis. Lastly, a logistic regression model investigated the regions potentially associated with FM, when controlling for sociodemographic variables and depressive symptoms. The analysis highlighted significant differences in the <i>GCSAML</i> region methylation between patients and controls. Moreover, seventeen single CpGs, belonging to other genes, were significantly different, however, only one cytosine related to <i>GCSAML</i> survived the correction for multiple comparisons. The network structure of methylation sites was different for each group; <i>GRM2</i> methylation represented a central node only for FM patients. Logistic regression revealed that depressive symptoms and DNA methylation in the <i>GRM2</i> region were significantly associated with FM risk. Our study encourages better exploration of <i>GCSAML</i> and <i>GRM2</i> functions and their possible role in FM affecting immune, inflammatory response, and central sensitization of pain. |
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DNA methylation changes in fibromyalgia suggest the role of the immune-inflammatory response and central sensitizationFibromyalgiaEpigeneticsBloodBiomarkersDNA methylationDepressionImmune systemPain managementScience & TechnologyFibromyalgia (FM) has been explained as a result of gene-environment interactions. The present study aims to verify DNA methylation differences in eleven candidate genome regions previously associated to FM, evaluating DNA methylation patterns as potential disease biomarkers. DNA methylation was analyzed through bisulfite sequencing, comparing 42 FM women and their 42 healthy sisters. The associations between the level of methylation in these regions were further explored through a network analysis. Lastly, a logistic regression model investigated the regions potentially associated with FM, when controlling for sociodemographic variables and depressive symptoms. The analysis highlighted significant differences in the <i>GCSAML</i> region methylation between patients and controls. Moreover, seventeen single CpGs, belonging to other genes, were significantly different, however, only one cytosine related to <i>GCSAML</i> survived the correction for multiple comparisons. The network structure of methylation sites was different for each group; <i>GRM2</i> methylation represented a central node only for FM patients. Logistic regression revealed that depressive symptoms and DNA methylation in the <i>GRM2</i> region were significantly associated with FM risk. Our study encourages better exploration of <i>GCSAML</i> and <i>GRM2</i> functions and their possible role in FM affecting immune, inflammatory response, and central sensitization of pain.This research was funded by the Spanish Government Funding (Ministerio de Economía y Competitividad: grant PSI2013-45818-R). MCG and LAN are part of the Center for Neuroplasticity and Pain (CNAP) which is supported by the Danish National Research Foundation (DNRF121).Multidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoGerra, Maria CarlaCarnevali, DavideOssola, PaoloGonzález-Villar, Alberto J.Pedersen, Inge SøkildeTriñanes, YolandaDonnini, ClaudiaManfredini, MatteoArendt-Nielsen, LarsCarrillo-de-la-Peña, Maria Teresa2021-10-272021-10-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/75921engGerra, M.C.; Carnevali, D.; Ossola, P.; González-Villar, A.; Pedersen, I.S.; Triñanes, Y.; Donnini, C.; Manfredini, M.; Arendt-Nielsen, L.; Carrillo-de-la-Peña, M.T. DNA Methylation Changes in Fibromyalgia Suggest the Role of the Immune-Inflammatory Response and Central Sensitization. J. Clin. Med. 2021, 10, 4992. https://doi.org/10.3390/jcm102149922077-038310.3390/jcm102149924992https://www.mdpi.com/2077-0383/10/21/4992info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:16:17Zoai:repositorium.sdum.uminho.pt:1822/75921Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:08:49.153996Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
DNA methylation changes in fibromyalgia suggest the role of the immune-inflammatory response and central sensitization |
title |
DNA methylation changes in fibromyalgia suggest the role of the immune-inflammatory response and central sensitization |
spellingShingle |
DNA methylation changes in fibromyalgia suggest the role of the immune-inflammatory response and central sensitization Gerra, Maria Carla Fibromyalgia Epigenetics Blood Biomarkers DNA methylation Depression Immune system Pain management Science & Technology |
title_short |
DNA methylation changes in fibromyalgia suggest the role of the immune-inflammatory response and central sensitization |
title_full |
DNA methylation changes in fibromyalgia suggest the role of the immune-inflammatory response and central sensitization |
title_fullStr |
DNA methylation changes in fibromyalgia suggest the role of the immune-inflammatory response and central sensitization |
title_full_unstemmed |
DNA methylation changes in fibromyalgia suggest the role of the immune-inflammatory response and central sensitization |
title_sort |
DNA methylation changes in fibromyalgia suggest the role of the immune-inflammatory response and central sensitization |
author |
Gerra, Maria Carla |
author_facet |
Gerra, Maria Carla Carnevali, Davide Ossola, Paolo González-Villar, Alberto J. Pedersen, Inge Søkilde Triñanes, Yolanda Donnini, Claudia Manfredini, Matteo Arendt-Nielsen, Lars Carrillo-de-la-Peña, Maria Teresa |
author_role |
author |
author2 |
Carnevali, Davide Ossola, Paolo González-Villar, Alberto J. Pedersen, Inge Søkilde Triñanes, Yolanda Donnini, Claudia Manfredini, Matteo Arendt-Nielsen, Lars Carrillo-de-la-Peña, Maria Teresa |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Gerra, Maria Carla Carnevali, Davide Ossola, Paolo González-Villar, Alberto J. Pedersen, Inge Søkilde Triñanes, Yolanda Donnini, Claudia Manfredini, Matteo Arendt-Nielsen, Lars Carrillo-de-la-Peña, Maria Teresa |
dc.subject.por.fl_str_mv |
Fibromyalgia Epigenetics Blood Biomarkers DNA methylation Depression Immune system Pain management Science & Technology |
topic |
Fibromyalgia Epigenetics Blood Biomarkers DNA methylation Depression Immune system Pain management Science & Technology |
description |
Fibromyalgia (FM) has been explained as a result of gene-environment interactions. The present study aims to verify DNA methylation differences in eleven candidate genome regions previously associated to FM, evaluating DNA methylation patterns as potential disease biomarkers. DNA methylation was analyzed through bisulfite sequencing, comparing 42 FM women and their 42 healthy sisters. The associations between the level of methylation in these regions were further explored through a network analysis. Lastly, a logistic regression model investigated the regions potentially associated with FM, when controlling for sociodemographic variables and depressive symptoms. The analysis highlighted significant differences in the <i>GCSAML</i> region methylation between patients and controls. Moreover, seventeen single CpGs, belonging to other genes, were significantly different, however, only one cytosine related to <i>GCSAML</i> survived the correction for multiple comparisons. The network structure of methylation sites was different for each group; <i>GRM2</i> methylation represented a central node only for FM patients. Logistic regression revealed that depressive symptoms and DNA methylation in the <i>GRM2</i> region were significantly associated with FM risk. Our study encourages better exploration of <i>GCSAML</i> and <i>GRM2</i> functions and their possible role in FM affecting immune, inflammatory response, and central sensitization of pain. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-27 2021-10-27T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/75921 |
url |
http://hdl.handle.net/1822/75921 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gerra, M.C.; Carnevali, D.; Ossola, P.; González-Villar, A.; Pedersen, I.S.; Triñanes, Y.; Donnini, C.; Manfredini, M.; Arendt-Nielsen, L.; Carrillo-de-la-Peña, M.T. DNA Methylation Changes in Fibromyalgia Suggest the Role of the Immune-Inflammatory Response and Central Sensitization. J. Clin. Med. 2021, 10, 4992. https://doi.org/10.3390/jcm10214992 2077-0383 10.3390/jcm10214992 4992 https://www.mdpi.com/2077-0383/10/21/4992 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132511665651712 |