Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/58894 |
Resumo: | Bacteriophages (phages) or viruses that specifically infect bacteria have widely been studied as biocontrol agents against animal and plant bacterial diseases. They offer many advantages compared to antibiotics. The American Foulbrood (AFB) is a bacterial disease affecting honeybee larvae caused by Paenibacillus larvae. Phages can be very significant in fighting it mostly due to European restrictions to the use of antibiotics in beekeeping. New phages able to control P. larvae in hives have already been reported with satisfactory results. However, the efficacy and feasibility of administering phages indirectly to larvae through their adult workers only by providing phages in bees feeders has never been evaluated. This strategy is considered herein the most feasible as far as hive management is concerned. This in vivo study investigated the ability of a phage to reach larvae in an infective state after oral administration to honeybees. The screening (by direct PFU count) and quantification (by quantitative PCR) of the phage in bee organs and in larvae after ingestion allowed us to conclude that despite 104 phages reaching larvae only an average of 32 were available to control the spread of the disease. The fast inactivation of many phages in royal jelly could compromise this therapeutic approach. The protection of phages from hive-derived conditions should be thus considered in further developments for AFB treatment. |
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Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage modelScience & TechnologyBacteriophages (phages) or viruses that specifically infect bacteria have widely been studied as biocontrol agents against animal and plant bacterial diseases. They offer many advantages compared to antibiotics. The American Foulbrood (AFB) is a bacterial disease affecting honeybee larvae caused by Paenibacillus larvae. Phages can be very significant in fighting it mostly due to European restrictions to the use of antibiotics in beekeeping. New phages able to control P. larvae in hives have already been reported with satisfactory results. However, the efficacy and feasibility of administering phages indirectly to larvae through their adult workers only by providing phages in bees feeders has never been evaluated. This strategy is considered herein the most feasible as far as hive management is concerned. This in vivo study investigated the ability of a phage to reach larvae in an infective state after oral administration to honeybees. The screening (by direct PFU count) and quantification (by quantitative PCR) of the phage in bee organs and in larvae after ingestion allowed us to conclude that despite 104 phages reaching larvae only an average of 32 were available to control the spread of the disease. The fast inactivation of many phages in royal jelly could compromise this therapeutic approach. The protection of phages from hive-derived conditions should be thus considered in further developments for AFB treatment.This study was supported by the project APILYSE, PTDC/CVT-EPI/4008/2014 - POCI-01-0145-FEDER-016598, - funded by FEDER through COMPETE 2020 - Programa Operacional Competitividade e Internacionalização (POCI) and by national funds trough FCT - Fundação para a Ciência e a Tecnologia, I.P. The work was also supported by the strategic funding of UID/BIO/04469/2013 unit, COMPETE 2020 (POCI-01-0145FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004), funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. HR was supported by FCT through the grant SFRH/BD/128859/2017. RC was founded by FCT and FEDER (POCI-010145-FEDER-007274).info:eu-repo/semantics/publishedVersionNature ResearchUniversidade do MinhoRibeiro, HenriqueCorreia, RossanaMoreira, TiagoBoas, Diana Patrícia Andrade VilasAzeredo, JoanaOliveira, A.2019-01-242019-01-24T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/58894engRibeiro, Henrique; Correia, Rossana; Moreira, Tiago; Vilas Boas, Diana; Azeredo, Joana; Oliveira, A., Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model. Scientific Reports, 9(1), 620-620, 20192045-23222045-232210.1038/s41598-018-36432-x30679452http://www.nature.com/srep/index.htmlinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:10:18Zoai:repositorium.sdum.uminho.pt:1822/58894Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:01:53.589702Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model |
title |
Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model |
spellingShingle |
Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model Ribeiro, Henrique Science & Technology |
title_short |
Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model |
title_full |
Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model |
title_fullStr |
Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model |
title_full_unstemmed |
Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model |
title_sort |
Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model |
author |
Ribeiro, Henrique |
author_facet |
Ribeiro, Henrique Correia, Rossana Moreira, Tiago Boas, Diana Patrícia Andrade Vilas Azeredo, Joana Oliveira, A. |
author_role |
author |
author2 |
Correia, Rossana Moreira, Tiago Boas, Diana Patrícia Andrade Vilas Azeredo, Joana Oliveira, A. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Ribeiro, Henrique Correia, Rossana Moreira, Tiago Boas, Diana Patrícia Andrade Vilas Azeredo, Joana Oliveira, A. |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
Bacteriophages (phages) or viruses that specifically infect bacteria have widely been studied as biocontrol agents against animal and plant bacterial diseases. They offer many advantages compared to antibiotics. The American Foulbrood (AFB) is a bacterial disease affecting honeybee larvae caused by Paenibacillus larvae. Phages can be very significant in fighting it mostly due to European restrictions to the use of antibiotics in beekeeping. New phages able to control P. larvae in hives have already been reported with satisfactory results. However, the efficacy and feasibility of administering phages indirectly to larvae through their adult workers only by providing phages in bees feeders has never been evaluated. This strategy is considered herein the most feasible as far as hive management is concerned. This in vivo study investigated the ability of a phage to reach larvae in an infective state after oral administration to honeybees. The screening (by direct PFU count) and quantification (by quantitative PCR) of the phage in bee organs and in larvae after ingestion allowed us to conclude that despite 104 phages reaching larvae only an average of 32 were available to control the spread of the disease. The fast inactivation of many phages in royal jelly could compromise this therapeutic approach. The protection of phages from hive-derived conditions should be thus considered in further developments for AFB treatment. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-24 2019-01-24T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/58894 |
url |
http://hdl.handle.net/1822/58894 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ribeiro, Henrique; Correia, Rossana; Moreira, Tiago; Vilas Boas, Diana; Azeredo, Joana; Oliveira, A., Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model. Scientific Reports, 9(1), 620-620, 2019 2045-2322 2045-2322 10.1038/s41598-018-36432-x 30679452 http://www.nature.com/srep/index.html |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature Research |
publisher.none.fl_str_mv |
Nature Research |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132419225288704 |