Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry

Detalhes bibliográficos
Autor(a) principal: Sillankorva, Sanna
Data de Publicação: 2010
Outros Autores: Pleteneva, E. A., Shaburova, O. V., Santos, Sílvio Roberto Branco, Carvalho, Carla A. O. C. M., Azeredo, Joana, Krylov, V. N.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/10691
Resumo: Aims: Salmonella is a worldwide foodborne pathogen causing acute enteric infections in humans. In the recent years, the use of bacteriophages has been suggested as a possible tool to combat this zoonotic pathogen in poultry farms. This work aims to isolate and perform comparative studies of a group of phages active against a collection of specific Salmonella Enteritidis strains from Portugal and England. Also, suitable phage candidates for therapy of poultry will be selected. Methods and Results: The Salm. Enteritidis strains studied were shown to have a significantly high occurrence of defective (cryptic) prophages; however, no live phages were found in the strains. Bacteriophages isolated from different environments lysed all except one of the tested Salm. Enteritidis strains. The bacteriophages studied were divided into different groups according to their genetic homology, RFLP profiles and phenotypic features, and most of them showed no DNA homology with the bacterial hosts. The bacteriophage lytic efficacy proved to be highly dependent on the propagation host strain. Conclusions: Despite the evidences shown in this work that the Salm. Enteritidis strains used did not produce viable phages, we have confirmed that some phages, when grown on particular hosts, behaved as complexes of phages. This is most likely because of the presence of inactive phage-related genomes (or their parts) in the bacterial strains which are capable of being reactivated or which can recombine with lytic phages. Furthermore, changes of the bacterial hosts used for maintenance of phages must be avoided as these can drastically modify the parameters of the phage preparations, including host range and lytic activity. Significance and Impact of the Study: This work shows that the optimal host and growth conditions must be carefully studied and selected for the production of each bacteriophage candidate for animal therapy.
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spelling Salmonella Enteritidis bacteriophage candidates for phage therapy of poultryBacteriophagePoultrySalmonella EnteritidisTherapyScience & TechnologyAims: Salmonella is a worldwide foodborne pathogen causing acute enteric infections in humans. In the recent years, the use of bacteriophages has been suggested as a possible tool to combat this zoonotic pathogen in poultry farms. This work aims to isolate and perform comparative studies of a group of phages active against a collection of specific Salmonella Enteritidis strains from Portugal and England. Also, suitable phage candidates for therapy of poultry will be selected. Methods and Results: The Salm. Enteritidis strains studied were shown to have a significantly high occurrence of defective (cryptic) prophages; however, no live phages were found in the strains. Bacteriophages isolated from different environments lysed all except one of the tested Salm. Enteritidis strains. The bacteriophages studied were divided into different groups according to their genetic homology, RFLP profiles and phenotypic features, and most of them showed no DNA homology with the bacterial hosts. The bacteriophage lytic efficacy proved to be highly dependent on the propagation host strain. Conclusions: Despite the evidences shown in this work that the Salm. Enteritidis strains used did not produce viable phages, we have confirmed that some phages, when grown on particular hosts, behaved as complexes of phages. This is most likely because of the presence of inactive phage-related genomes (or their parts) in the bacterial strains which are capable of being reactivated or which can recombine with lytic phages. Furthermore, changes of the bacterial hosts used for maintenance of phages must be avoided as these can drastically modify the parameters of the phage preparations, including host range and lytic activity. Significance and Impact of the Study: This work shows that the optimal host and growth conditions must be carefully studied and selected for the production of each bacteriophage candidate for animal therapy.The authors kindly acknowledge the Instituto Nacional Ricardo Jorge (INRJ) (Portugal) and also Tom Humphrey from the Division of Farm Animal Science, School of Clinical Veterinary Science of the University of Bristol (UK) for providing the Salm. Enteritidis isolates used in this work.The Society for Applied MicrobiologyUniversidade do MinhoSillankorva, SannaPleteneva, E. A.Shaburova, O. V.Santos, Sílvio Roberto BrancoCarvalho, Carla A. O. C. M.Azeredo, JoanaKrylov, V. N.2010-042010-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/10691eng"Journal of Applied Microbiology". ISSN 1364-5072. 108:4 (2010) 1175-1186.1364-507210.1111/j.1365-2672.2009.04549.x19796092info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:40:42Zoai:repositorium.sdum.uminho.pt:1822/10691Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:37:33.324522Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry
title Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry
spellingShingle Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry
Sillankorva, Sanna
Bacteriophage
Poultry
Salmonella Enteritidis
Therapy
Science & Technology
title_short Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry
title_full Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry
title_fullStr Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry
title_full_unstemmed Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry
title_sort Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry
author Sillankorva, Sanna
author_facet Sillankorva, Sanna
Pleteneva, E. A.
Shaburova, O. V.
Santos, Sílvio Roberto Branco
Carvalho, Carla A. O. C. M.
Azeredo, Joana
Krylov, V. N.
author_role author
author2 Pleteneva, E. A.
Shaburova, O. V.
Santos, Sílvio Roberto Branco
Carvalho, Carla A. O. C. M.
Azeredo, Joana
Krylov, V. N.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Sillankorva, Sanna
Pleteneva, E. A.
Shaburova, O. V.
Santos, Sílvio Roberto Branco
Carvalho, Carla A. O. C. M.
Azeredo, Joana
Krylov, V. N.
dc.subject.por.fl_str_mv Bacteriophage
Poultry
Salmonella Enteritidis
Therapy
Science & Technology
topic Bacteriophage
Poultry
Salmonella Enteritidis
Therapy
Science & Technology
description Aims: Salmonella is a worldwide foodborne pathogen causing acute enteric infections in humans. In the recent years, the use of bacteriophages has been suggested as a possible tool to combat this zoonotic pathogen in poultry farms. This work aims to isolate and perform comparative studies of a group of phages active against a collection of specific Salmonella Enteritidis strains from Portugal and England. Also, suitable phage candidates for therapy of poultry will be selected. Methods and Results: The Salm. Enteritidis strains studied were shown to have a significantly high occurrence of defective (cryptic) prophages; however, no live phages were found in the strains. Bacteriophages isolated from different environments lysed all except one of the tested Salm. Enteritidis strains. The bacteriophages studied were divided into different groups according to their genetic homology, RFLP profiles and phenotypic features, and most of them showed no DNA homology with the bacterial hosts. The bacteriophage lytic efficacy proved to be highly dependent on the propagation host strain. Conclusions: Despite the evidences shown in this work that the Salm. Enteritidis strains used did not produce viable phages, we have confirmed that some phages, when grown on particular hosts, behaved as complexes of phages. This is most likely because of the presence of inactive phage-related genomes (or their parts) in the bacterial strains which are capable of being reactivated or which can recombine with lytic phages. Furthermore, changes of the bacterial hosts used for maintenance of phages must be avoided as these can drastically modify the parameters of the phage preparations, including host range and lytic activity. Significance and Impact of the Study: This work shows that the optimal host and growth conditions must be carefully studied and selected for the production of each bacteriophage candidate for animal therapy.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
2010-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/10691
url http://hdl.handle.net/1822/10691
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Journal of Applied Microbiology". ISSN 1364-5072. 108:4 (2010) 1175-1186.
1364-5072
10.1111/j.1365-2672.2009.04549.x
19796092
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Society for Applied Microbiology
publisher.none.fl_str_mv The Society for Applied Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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