Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/10691 |
Resumo: | Aims: Salmonella is a worldwide foodborne pathogen causing acute enteric infections in humans. In the recent years, the use of bacteriophages has been suggested as a possible tool to combat this zoonotic pathogen in poultry farms. This work aims to isolate and perform comparative studies of a group of phages active against a collection of specific Salmonella Enteritidis strains from Portugal and England. Also, suitable phage candidates for therapy of poultry will be selected. Methods and Results: The Salm. Enteritidis strains studied were shown to have a significantly high occurrence of defective (cryptic) prophages; however, no live phages were found in the strains. Bacteriophages isolated from different environments lysed all except one of the tested Salm. Enteritidis strains. The bacteriophages studied were divided into different groups according to their genetic homology, RFLP profiles and phenotypic features, and most of them showed no DNA homology with the bacterial hosts. The bacteriophage lytic efficacy proved to be highly dependent on the propagation host strain. Conclusions: Despite the evidences shown in this work that the Salm. Enteritidis strains used did not produce viable phages, we have confirmed that some phages, when grown on particular hosts, behaved as complexes of phages. This is most likely because of the presence of inactive phage-related genomes (or their parts) in the bacterial strains which are capable of being reactivated or which can recombine with lytic phages. Furthermore, changes of the bacterial hosts used for maintenance of phages must be avoided as these can drastically modify the parameters of the phage preparations, including host range and lytic activity. Significance and Impact of the Study: This work shows that the optimal host and growth conditions must be carefully studied and selected for the production of each bacteriophage candidate for animal therapy. |
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Salmonella Enteritidis bacteriophage candidates for phage therapy of poultryBacteriophagePoultrySalmonella EnteritidisTherapyScience & TechnologyAims: Salmonella is a worldwide foodborne pathogen causing acute enteric infections in humans. In the recent years, the use of bacteriophages has been suggested as a possible tool to combat this zoonotic pathogen in poultry farms. This work aims to isolate and perform comparative studies of a group of phages active against a collection of specific Salmonella Enteritidis strains from Portugal and England. Also, suitable phage candidates for therapy of poultry will be selected. Methods and Results: The Salm. Enteritidis strains studied were shown to have a significantly high occurrence of defective (cryptic) prophages; however, no live phages were found in the strains. Bacteriophages isolated from different environments lysed all except one of the tested Salm. Enteritidis strains. The bacteriophages studied were divided into different groups according to their genetic homology, RFLP profiles and phenotypic features, and most of them showed no DNA homology with the bacterial hosts. The bacteriophage lytic efficacy proved to be highly dependent on the propagation host strain. Conclusions: Despite the evidences shown in this work that the Salm. Enteritidis strains used did not produce viable phages, we have confirmed that some phages, when grown on particular hosts, behaved as complexes of phages. This is most likely because of the presence of inactive phage-related genomes (or their parts) in the bacterial strains which are capable of being reactivated or which can recombine with lytic phages. Furthermore, changes of the bacterial hosts used for maintenance of phages must be avoided as these can drastically modify the parameters of the phage preparations, including host range and lytic activity. Significance and Impact of the Study: This work shows that the optimal host and growth conditions must be carefully studied and selected for the production of each bacteriophage candidate for animal therapy.The authors kindly acknowledge the Instituto Nacional Ricardo Jorge (INRJ) (Portugal) and also Tom Humphrey from the Division of Farm Animal Science, School of Clinical Veterinary Science of the University of Bristol (UK) for providing the Salm. Enteritidis isolates used in this work.The Society for Applied MicrobiologyUniversidade do MinhoSillankorva, SannaPleteneva, E. A.Shaburova, O. V.Santos, Sílvio Roberto BrancoCarvalho, Carla A. O. C. M.Azeredo, JoanaKrylov, V. N.2010-042010-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/10691eng"Journal of Applied Microbiology". ISSN 1364-5072. 108:4 (2010) 1175-1186.1364-507210.1111/j.1365-2672.2009.04549.x19796092info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:40:42Zoai:repositorium.sdum.uminho.pt:1822/10691Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:37:33.324522Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry |
title |
Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry |
spellingShingle |
Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry Sillankorva, Sanna Bacteriophage Poultry Salmonella Enteritidis Therapy Science & Technology |
title_short |
Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry |
title_full |
Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry |
title_fullStr |
Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry |
title_full_unstemmed |
Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry |
title_sort |
Salmonella Enteritidis bacteriophage candidates for phage therapy of poultry |
author |
Sillankorva, Sanna |
author_facet |
Sillankorva, Sanna Pleteneva, E. A. Shaburova, O. V. Santos, Sílvio Roberto Branco Carvalho, Carla A. O. C. M. Azeredo, Joana Krylov, V. N. |
author_role |
author |
author2 |
Pleteneva, E. A. Shaburova, O. V. Santos, Sílvio Roberto Branco Carvalho, Carla A. O. C. M. Azeredo, Joana Krylov, V. N. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Sillankorva, Sanna Pleteneva, E. A. Shaburova, O. V. Santos, Sílvio Roberto Branco Carvalho, Carla A. O. C. M. Azeredo, Joana Krylov, V. N. |
dc.subject.por.fl_str_mv |
Bacteriophage Poultry Salmonella Enteritidis Therapy Science & Technology |
topic |
Bacteriophage Poultry Salmonella Enteritidis Therapy Science & Technology |
description |
Aims: Salmonella is a worldwide foodborne pathogen causing acute enteric infections in humans. In the recent years, the use of bacteriophages has been suggested as a possible tool to combat this zoonotic pathogen in poultry farms. This work aims to isolate and perform comparative studies of a group of phages active against a collection of specific Salmonella Enteritidis strains from Portugal and England. Also, suitable phage candidates for therapy of poultry will be selected. Methods and Results: The Salm. Enteritidis strains studied were shown to have a significantly high occurrence of defective (cryptic) prophages; however, no live phages were found in the strains. Bacteriophages isolated from different environments lysed all except one of the tested Salm. Enteritidis strains. The bacteriophages studied were divided into different groups according to their genetic homology, RFLP profiles and phenotypic features, and most of them showed no DNA homology with the bacterial hosts. The bacteriophage lytic efficacy proved to be highly dependent on the propagation host strain. Conclusions: Despite the evidences shown in this work that the Salm. Enteritidis strains used did not produce viable phages, we have confirmed that some phages, when grown on particular hosts, behaved as complexes of phages. This is most likely because of the presence of inactive phage-related genomes (or their parts) in the bacterial strains which are capable of being reactivated or which can recombine with lytic phages. Furthermore, changes of the bacterial hosts used for maintenance of phages must be avoided as these can drastically modify the parameters of the phage preparations, including host range and lytic activity. Significance and Impact of the Study: This work shows that the optimal host and growth conditions must be carefully studied and selected for the production of each bacteriophage candidate for animal therapy. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-04 2010-04-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/10691 |
url |
http://hdl.handle.net/1822/10691 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Journal of Applied Microbiology". ISSN 1364-5072. 108:4 (2010) 1175-1186. 1364-5072 10.1111/j.1365-2672.2009.04549.x 19796092 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
The Society for Applied Microbiology |
publisher.none.fl_str_mv |
The Society for Applied Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132909411500032 |