Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in the measurement of saliva analytes
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/35137 https://doi.org/10.1038/s41598-022-13491-9. https://doi.org/10.1038/s41598-022-13491-9 |
Resumo: | The present study aims to assess the effects of thermal and chemical inactivating procedures, that can be used for SARS-CoV-2 inactivation, on different salivary analytes. SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) protein profile and a panel of 25 specific biomarkers of oxidative status, stress, metabolism and tissue damage were evaluated in samples subjected to different treatments: thermal (65 °C or 92 °C) and chemical with detergents [sodium dodecyl sulphate (SDS), Triton X-100 or NP-40]. Salivary SDS-PAGE profile was most affected by heating at 92 °C, with three and two protein bands decreasing and increasing their expression levels, respectively. This treatment also affected the results of several enzymes, with some of them being also affected by heating at 65 °C and incubation with SDS. The use of Triton X-100 or NP-40 resulted in increased values of cortisol, triglycerides and glucose, not affecting the other tested biomarkers. The present results will help researchers and clinicians to select the best protocols to work in safe conditions with saliva, taking into account the target analyte planned to be measured. |
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Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in the measurement of saliva analytesThermal and chemical treatmentsSARS-COV-2Saliva analytesThe present study aims to assess the effects of thermal and chemical inactivating procedures, that can be used for SARS-CoV-2 inactivation, on different salivary analytes. SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) protein profile and a panel of 25 specific biomarkers of oxidative status, stress, metabolism and tissue damage were evaluated in samples subjected to different treatments: thermal (65 °C or 92 °C) and chemical with detergents [sodium dodecyl sulphate (SDS), Triton X-100 or NP-40]. Salivary SDS-PAGE profile was most affected by heating at 92 °C, with three and two protein bands decreasing and increasing their expression levels, respectively. This treatment also affected the results of several enzymes, with some of them being also affected by heating at 65 °C and incubation with SDS. The use of Triton X-100 or NP-40 resulted in increased values of cortisol, triglycerides and glucose, not affecting the other tested biomarkers. The present results will help researchers and clinicians to select the best protocols to work in safe conditions with saliva, taking into account the target analyte planned to be measured.Springer Nature2023-05-17T13:09:04Z2023-05-172022-06-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/35137https://doi.org/10.1038/s41598-022-13491-9.http://hdl.handle.net/10174/35137https://doi.org/10.1038/s41598-022-13491-9porLamy E, Rubio CP, Carreira L, Capela E Silva F, Martinez-Subiela S, Tecles F, Lopez-Jornet P, Ceron JJ, Tvarijonaviciute A. Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in the measurement of saliva analytes. Sci Rep. 2022 Jun 8;12(1):9434. doi: 10.1038/s41598-022-13491-9.https://www.nature.com/articles/s41598-022-13491-9Scientific ReportsMEDecsl@uevora.ptndlrec@uevora.ptfcs@uevora.ptndndndndnd235Lamy, ElsaRubio, CamilaCarreira, LauraCapela e Silva, FernandoMartinez-Subiela, SilviaTecles, FernandoLopez-Jornet, PiaCéron, JoseTvarijonaviciute, Astainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:38:21Zoai:dspace.uevora.pt:10174/35137Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:23:34.289271Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in the measurement of saliva analytes |
title |
Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in the measurement of saliva analytes |
spellingShingle |
Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in the measurement of saliva analytes Lamy, Elsa Thermal and chemical treatments SARS-COV-2 Saliva analytes |
title_short |
Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in the measurement of saliva analytes |
title_full |
Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in the measurement of saliva analytes |
title_fullStr |
Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in the measurement of saliva analytes |
title_full_unstemmed |
Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in the measurement of saliva analytes |
title_sort |
Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in the measurement of saliva analytes |
author |
Lamy, Elsa |
author_facet |
Lamy, Elsa Rubio, Camila Carreira, Laura Capela e Silva, Fernando Martinez-Subiela, Silvia Tecles, Fernando Lopez-Jornet, Pia Céron, Jose Tvarijonaviciute, Asta |
author_role |
author |
author2 |
Rubio, Camila Carreira, Laura Capela e Silva, Fernando Martinez-Subiela, Silvia Tecles, Fernando Lopez-Jornet, Pia Céron, Jose Tvarijonaviciute, Asta |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lamy, Elsa Rubio, Camila Carreira, Laura Capela e Silva, Fernando Martinez-Subiela, Silvia Tecles, Fernando Lopez-Jornet, Pia Céron, Jose Tvarijonaviciute, Asta |
dc.subject.por.fl_str_mv |
Thermal and chemical treatments SARS-COV-2 Saliva analytes |
topic |
Thermal and chemical treatments SARS-COV-2 Saliva analytes |
description |
The present study aims to assess the effects of thermal and chemical inactivating procedures, that can be used for SARS-CoV-2 inactivation, on different salivary analytes. SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) protein profile and a panel of 25 specific biomarkers of oxidative status, stress, metabolism and tissue damage were evaluated in samples subjected to different treatments: thermal (65 °C or 92 °C) and chemical with detergents [sodium dodecyl sulphate (SDS), Triton X-100 or NP-40]. Salivary SDS-PAGE profile was most affected by heating at 92 °C, with three and two protein bands decreasing and increasing their expression levels, respectively. This treatment also affected the results of several enzymes, with some of them being also affected by heating at 65 °C and incubation with SDS. The use of Triton X-100 or NP-40 resulted in increased values of cortisol, triglycerides and glucose, not affecting the other tested biomarkers. The present results will help researchers and clinicians to select the best protocols to work in safe conditions with saliva, taking into account the target analyte planned to be measured. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-06-08T00:00:00Z 2023-05-17T13:09:04Z 2023-05-17 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/35137 https://doi.org/10.1038/s41598-022-13491-9. http://hdl.handle.net/10174/35137 https://doi.org/10.1038/s41598-022-13491-9 |
url |
http://hdl.handle.net/10174/35137 https://doi.org/10.1038/s41598-022-13491-9. https://doi.org/10.1038/s41598-022-13491-9 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Lamy E, Rubio CP, Carreira L, Capela E Silva F, Martinez-Subiela S, Tecles F, Lopez-Jornet P, Ceron JJ, Tvarijonaviciute A. Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in the measurement of saliva analytes. Sci Rep. 2022 Jun 8;12(1):9434. doi: 10.1038/s41598-022-13491-9. https://www.nature.com/articles/s41598-022-13491-9 Scientific Reports MED ecsl@uevora.pt nd lrec@uevora.pt fcs@uevora.pt nd nd nd nd nd 235 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Springer Nature |
publisher.none.fl_str_mv |
Springer Nature |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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