Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance

Detalhes bibliográficos
Autor(a) principal: Vicente-Martins, Sofia
Data de Publicação: 2018
Outros Autores: Oleastro, Mónica, Domingues, Fernanda C., Ferreira, Susana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/6338
Resumo: Arcobacter genus is considered a foodborne emergent pathogen able to cause disease among humans and animals. The wide distribution and high prevalence of Arcobacter in food is becoming a concern, since consumption of contaminated food and water is considered the most probable route of Arcobacter transmission to humans. In this work, ready-to-eat packaged vegetables, poultry, pork, beef and fish meat were purchased from several retail markets and supermarkets and tested for the presence of Arcobacter spp. Suspected colonies were isolated and identified, characterized through ERIC-PCR and resistance to nine antibiotics commonly used to treat infections by this microorganism was assessed; in parallel, Arcobacter spp. was directly detected on enrichment broth by multiplex PCR. The results show a high prevalence of Arcobacter spp. among retail food, with an overall prevalence of 60.5% obtained by both molecular and culture detection. A. butzleri was the most frequently isolated species (58.5%) followed by A. cryaerophilus (35.8%) and A. skirrowii (5.7%). A high genetic diversity among the isolates was observed, despite the detection of a possible cross-contamination between food samples. Also, a high rate of multidrug resistance among the isolates (85.7%) was observed. Taken together, our results suggest that the consumption of Arcobacter-contaminated food products is of public health concern.
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spelling Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistanceGastrointestinal InfectionsArcobacter spp.Retail Food ProductsPortugalPrevalenceGenotypingCross-contaminationReady-to-eat FoodAntimicrobial ResistanceInfecções GastrointestinaisArcobacter genus is considered a foodborne emergent pathogen able to cause disease among humans and animals. The wide distribution and high prevalence of Arcobacter in food is becoming a concern, since consumption of contaminated food and water is considered the most probable route of Arcobacter transmission to humans. In this work, ready-to-eat packaged vegetables, poultry, pork, beef and fish meat were purchased from several retail markets and supermarkets and tested for the presence of Arcobacter spp. Suspected colonies were isolated and identified, characterized through ERIC-PCR and resistance to nine antibiotics commonly used to treat infections by this microorganism was assessed; in parallel, Arcobacter spp. was directly detected on enrichment broth by multiplex PCR. The results show a high prevalence of Arcobacter spp. among retail food, with an overall prevalence of 60.5% obtained by both molecular and culture detection. A. butzleri was the most frequently isolated species (58.5%) followed by A. cryaerophilus (35.8%) and A. skirrowii (5.7%). A high genetic diversity among the isolates was observed, despite the detection of a possible cross-contamination between food samples. Also, a high rate of multidrug resistance among the isolates (85.7%) was observed. Taken together, our results suggest that the consumption of Arcobacter-contaminated food products is of public health concern.Susana Ferreira was supported by a fellowship (SFRH/BPD/101959/2014) from Fundação para a Ciência e Tecnologia and co-financed by Fundo Social Europeu. This work was supported by FEDER funds through the POCI - COMPETE 2020 - Operational Programme Competitiveness and Internationalisation in Axis I - Strengthening research, technological development and innovation (Project POCI-01-0145-FEDER-007491) and National Funds by FCT - Foundation for Science and Technology (Project UID/Multi/00709/2013).Elsevier/ European Federation of Food Science and Technology (EFFoST)Repositório Científico do Instituto Nacional de SaúdeVicente-Martins, SofiaOleastro, MónicaDomingues, Fernanda C.Ferreira, Susana2019-03-28T18:37:34Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6338engFood Control. 2018;85:107-112. doi:10.1016/j.foodcont.2017.09.0240956-713510.1016/j.foodcont.2017.09.024info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:22Zoai:repositorio.insa.pt:10400.18/6338Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:40:59.266250Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance
title Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance
spellingShingle Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance
Vicente-Martins, Sofia
Gastrointestinal Infections
Arcobacter spp.
Retail Food Products
Portugal
Prevalence
Genotyping
Cross-contamination
Ready-to-eat Food
Antimicrobial Resistance
Infecções Gastrointestinais
title_short Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance
title_full Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance
title_fullStr Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance
title_full_unstemmed Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance
title_sort Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance
author Vicente-Martins, Sofia
author_facet Vicente-Martins, Sofia
Oleastro, Mónica
Domingues, Fernanda C.
Ferreira, Susana
author_role author
author2 Oleastro, Mónica
Domingues, Fernanda C.
Ferreira, Susana
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Vicente-Martins, Sofia
Oleastro, Mónica
Domingues, Fernanda C.
Ferreira, Susana
dc.subject.por.fl_str_mv Gastrointestinal Infections
Arcobacter spp.
Retail Food Products
Portugal
Prevalence
Genotyping
Cross-contamination
Ready-to-eat Food
Antimicrobial Resistance
Infecções Gastrointestinais
topic Gastrointestinal Infections
Arcobacter spp.
Retail Food Products
Portugal
Prevalence
Genotyping
Cross-contamination
Ready-to-eat Food
Antimicrobial Resistance
Infecções Gastrointestinais
description Arcobacter genus is considered a foodborne emergent pathogen able to cause disease among humans and animals. The wide distribution and high prevalence of Arcobacter in food is becoming a concern, since consumption of contaminated food and water is considered the most probable route of Arcobacter transmission to humans. In this work, ready-to-eat packaged vegetables, poultry, pork, beef and fish meat were purchased from several retail markets and supermarkets and tested for the presence of Arcobacter spp. Suspected colonies were isolated and identified, characterized through ERIC-PCR and resistance to nine antibiotics commonly used to treat infections by this microorganism was assessed; in parallel, Arcobacter spp. was directly detected on enrichment broth by multiplex PCR. The results show a high prevalence of Arcobacter spp. among retail food, with an overall prevalence of 60.5% obtained by both molecular and culture detection. A. butzleri was the most frequently isolated species (58.5%) followed by A. cryaerophilus (35.8%) and A. skirrowii (5.7%). A high genetic diversity among the isolates was observed, despite the detection of a possible cross-contamination between food samples. Also, a high rate of multidrug resistance among the isolates (85.7%) was observed. Taken together, our results suggest that the consumption of Arcobacter-contaminated food products is of public health concern.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
2019-03-28T18:37:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/6338
url http://hdl.handle.net/10400.18/6338
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Food Control. 2018;85:107-112. doi:10.1016/j.foodcont.2017.09.024
0956-7135
10.1016/j.foodcont.2017.09.024
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier/ European Federation of Food Science and Technology (EFFoST)
publisher.none.fl_str_mv Elsevier/ European Federation of Food Science and Technology (EFFoST)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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