Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/6338 |
Resumo: | Arcobacter genus is considered a foodborne emergent pathogen able to cause disease among humans and animals. The wide distribution and high prevalence of Arcobacter in food is becoming a concern, since consumption of contaminated food and water is considered the most probable route of Arcobacter transmission to humans. In this work, ready-to-eat packaged vegetables, poultry, pork, beef and fish meat were purchased from several retail markets and supermarkets and tested for the presence of Arcobacter spp. Suspected colonies were isolated and identified, characterized through ERIC-PCR and resistance to nine antibiotics commonly used to treat infections by this microorganism was assessed; in parallel, Arcobacter spp. was directly detected on enrichment broth by multiplex PCR. The results show a high prevalence of Arcobacter spp. among retail food, with an overall prevalence of 60.5% obtained by both molecular and culture detection. A. butzleri was the most frequently isolated species (58.5%) followed by A. cryaerophilus (35.8%) and A. skirrowii (5.7%). A high genetic diversity among the isolates was observed, despite the detection of a possible cross-contamination between food samples. Also, a high rate of multidrug resistance among the isolates (85.7%) was observed. Taken together, our results suggest that the consumption of Arcobacter-contaminated food products is of public health concern. |
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Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistanceGastrointestinal InfectionsArcobacter spp.Retail Food ProductsPortugalPrevalenceGenotypingCross-contaminationReady-to-eat FoodAntimicrobial ResistanceInfecções GastrointestinaisArcobacter genus is considered a foodborne emergent pathogen able to cause disease among humans and animals. The wide distribution and high prevalence of Arcobacter in food is becoming a concern, since consumption of contaminated food and water is considered the most probable route of Arcobacter transmission to humans. In this work, ready-to-eat packaged vegetables, poultry, pork, beef and fish meat were purchased from several retail markets and supermarkets and tested for the presence of Arcobacter spp. Suspected colonies were isolated and identified, characterized through ERIC-PCR and resistance to nine antibiotics commonly used to treat infections by this microorganism was assessed; in parallel, Arcobacter spp. was directly detected on enrichment broth by multiplex PCR. The results show a high prevalence of Arcobacter spp. among retail food, with an overall prevalence of 60.5% obtained by both molecular and culture detection. A. butzleri was the most frequently isolated species (58.5%) followed by A. cryaerophilus (35.8%) and A. skirrowii (5.7%). A high genetic diversity among the isolates was observed, despite the detection of a possible cross-contamination between food samples. Also, a high rate of multidrug resistance among the isolates (85.7%) was observed. Taken together, our results suggest that the consumption of Arcobacter-contaminated food products is of public health concern.Susana Ferreira was supported by a fellowship (SFRH/BPD/101959/2014) from Fundação para a Ciência e Tecnologia and co-financed by Fundo Social Europeu. This work was supported by FEDER funds through the POCI - COMPETE 2020 - Operational Programme Competitiveness and Internationalisation in Axis I - Strengthening research, technological development and innovation (Project POCI-01-0145-FEDER-007491) and National Funds by FCT - Foundation for Science and Technology (Project UID/Multi/00709/2013).Elsevier/ European Federation of Food Science and Technology (EFFoST)Repositório Científico do Instituto Nacional de SaúdeVicente-Martins, SofiaOleastro, MónicaDomingues, Fernanda C.Ferreira, Susana2019-03-28T18:37:34Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6338engFood Control. 2018;85:107-112. doi:10.1016/j.foodcont.2017.09.0240956-713510.1016/j.foodcont.2017.09.024info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:22Zoai:repositorio.insa.pt:10400.18/6338Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:40:59.266250Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance |
title |
Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance |
spellingShingle |
Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance Vicente-Martins, Sofia Gastrointestinal Infections Arcobacter spp. Retail Food Products Portugal Prevalence Genotyping Cross-contamination Ready-to-eat Food Antimicrobial Resistance Infecções Gastrointestinais |
title_short |
Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance |
title_full |
Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance |
title_fullStr |
Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance |
title_full_unstemmed |
Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance |
title_sort |
Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance |
author |
Vicente-Martins, Sofia |
author_facet |
Vicente-Martins, Sofia Oleastro, Mónica Domingues, Fernanda C. Ferreira, Susana |
author_role |
author |
author2 |
Oleastro, Mónica Domingues, Fernanda C. Ferreira, Susana |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Vicente-Martins, Sofia Oleastro, Mónica Domingues, Fernanda C. Ferreira, Susana |
dc.subject.por.fl_str_mv |
Gastrointestinal Infections Arcobacter spp. Retail Food Products Portugal Prevalence Genotyping Cross-contamination Ready-to-eat Food Antimicrobial Resistance Infecções Gastrointestinais |
topic |
Gastrointestinal Infections Arcobacter spp. Retail Food Products Portugal Prevalence Genotyping Cross-contamination Ready-to-eat Food Antimicrobial Resistance Infecções Gastrointestinais |
description |
Arcobacter genus is considered a foodborne emergent pathogen able to cause disease among humans and animals. The wide distribution and high prevalence of Arcobacter in food is becoming a concern, since consumption of contaminated food and water is considered the most probable route of Arcobacter transmission to humans. In this work, ready-to-eat packaged vegetables, poultry, pork, beef and fish meat were purchased from several retail markets and supermarkets and tested for the presence of Arcobacter spp. Suspected colonies were isolated and identified, characterized through ERIC-PCR and resistance to nine antibiotics commonly used to treat infections by this microorganism was assessed; in parallel, Arcobacter spp. was directly detected on enrichment broth by multiplex PCR. The results show a high prevalence of Arcobacter spp. among retail food, with an overall prevalence of 60.5% obtained by both molecular and culture detection. A. butzleri was the most frequently isolated species (58.5%) followed by A. cryaerophilus (35.8%) and A. skirrowii (5.7%). A high genetic diversity among the isolates was observed, despite the detection of a possible cross-contamination between food samples. Also, a high rate of multidrug resistance among the isolates (85.7%) was observed. Taken together, our results suggest that the consumption of Arcobacter-contaminated food products is of public health concern. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2018-01-01T00:00:00Z 2019-03-28T18:37:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/6338 |
url |
http://hdl.handle.net/10400.18/6338 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Food Control. 2018;85:107-112. doi:10.1016/j.foodcont.2017.09.024 0956-7135 10.1016/j.foodcont.2017.09.024 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier/ European Federation of Food Science and Technology (EFFoST) |
publisher.none.fl_str_mv |
Elsevier/ European Federation of Food Science and Technology (EFFoST) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132153062096896 |