The involvement of CDH1 in cancer angiogenesis

Detalhes bibliográficos
Autor(a) principal: Marques, Filipa Gil
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/6645
Resumo: Dissertação para obtenção do Grau de Mestre em Biotecnologia
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spelling The involvement of CDH1 in cancer angiogenesisCancerE-cadherin(Tumour-) AngiogenesisVEGF-ADissertação para obtenção do Grau de Mestre em BiotecnologiaCancer is one of the leading causes of death worldwide. Biological hallmarks such as induced and sustained angiogenesis are implicated in tumour progression, as well as invasion and metastasis which are the major causes of cancer-related mortality. E-Cadherin impairment on the cell membrane is intimately related with invasion and metastasis. Also, increased levels of vascular endothelial growth factor (VEGF), an angiogenic marker, and its receptor on the plasma membrane can be implicated in tumour progression. This work was focused on how the inactivation of E-Cadherin, a molecule associated to an invasive phenotype can be related with angiogenesis, probably through VEGF-A expression. Two different cell lines without expression of E-Cadherin and stably transduced to express wild-type (WT) E-Cadherin were used to carry out this study: AGS Par/WT (from stomach) and MDA-435 Mock/WT (from breast). Immunohistochemical staining was performed to determine the cellular localization and western blot analysis was performed to assess the expression levels of E-Cadherin. VEGFA mRNA levels were assessed by quantitative Real-time PCR. Additionally, we determined the levels phosphorylated (phospho) ERK1/2, as well as the expression levels of total ERK1/2. To study the angiogenic role of E-cadherin the chick embryo Chorioallantoic Membrane (CAM) assay was used. We characterise in vivo the different cell lines concerning both angiogenic and tumorigenic responses dependent on E-Cadherin. Only cell lines stably expressing WT human E-Cadherin showed levels of expression of this protein at the cell membrane regardless of their tissue of origin. In vitro, AGS and MDA-435 cells expressing WT E-Cadherin revealed an increased expression of VEGFA in comparison to the control although not statically significant. In addition, both phospho-ERK1/2 and total ERK1/2 presented similar levels of expression regardless of the tissue of origin and E-Cadherin expression. Both angiogenic and tumorigenic responses in AGS WT was significantly increased in comparison to the control. The MDA-435 WT cells revealed increased tumorigenic response in comparison to the control. Overall, these results suggest that E-Cadherin expression is important for micro-tumour formation as well as for neovascularisation but this effect is dependent on the in vivo context.Faculdade de Ciências e TecnologiaSeruca, RaquelRUNMarques, Filipa Gil2012-01-10T12:41:50Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/6645enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:37:49Zoai:run.unl.pt:10362/6645Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:16:54.896326Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The involvement of CDH1 in cancer angiogenesis
title The involvement of CDH1 in cancer angiogenesis
spellingShingle The involvement of CDH1 in cancer angiogenesis
Marques, Filipa Gil
Cancer
E-cadherin
(Tumour-) Angiogenesis
VEGF-A
title_short The involvement of CDH1 in cancer angiogenesis
title_full The involvement of CDH1 in cancer angiogenesis
title_fullStr The involvement of CDH1 in cancer angiogenesis
title_full_unstemmed The involvement of CDH1 in cancer angiogenesis
title_sort The involvement of CDH1 in cancer angiogenesis
author Marques, Filipa Gil
author_facet Marques, Filipa Gil
author_role author
dc.contributor.none.fl_str_mv Seruca, Raquel
RUN
dc.contributor.author.fl_str_mv Marques, Filipa Gil
dc.subject.por.fl_str_mv Cancer
E-cadherin
(Tumour-) Angiogenesis
VEGF-A
topic Cancer
E-cadherin
(Tumour-) Angiogenesis
VEGF-A
description Dissertação para obtenção do Grau de Mestre em Biotecnologia
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
2012-01-10T12:41:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/6645
url http://hdl.handle.net/10362/6645
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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