The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.1371/journal.pone.0178856 |
Resumo: | Fundacao para a Ciencia e a Tecnologia (FCT) through grant ERA-PTG/0005/2010 (in the frame of ERA-NET PathoGenoMics) to LJM and by Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), which is financed by national funds from FCT (UID/Multi/04378/2013) and co-financed by the European Regional Development Fund (ERDF) under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). MdC was supported by PhD fellowship SFRH/BD/62728/2009 from FCT. SVP and JNB hold PhD fellowships PD/BD/52210/2013 and PD/BD/128214/2016, respectively, within the scope of the PhD program Molecular Biosciences (PD/00133/2012) funded by FCT. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusionTRANSCRIPTIONAL REGULATORYERSINIA-ENTEROCOLITICATRANSLOCATED PROTEININC PROTEINSBiochemistry, Genetics and Molecular Biology(all)Agricultural and Biological Sciences(all)Fundacao para a Ciencia e a Tecnologia (FCT) through grant ERA-PTG/0005/2010 (in the frame of ERA-NET PathoGenoMics) to LJM and by Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), which is financed by national funds from FCT (UID/Multi/04378/2013) and co-financed by the European Regional Development Fund (ERDF) under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). MdC was supported by PhD fellowship SFRH/BD/62728/2009 from FCT. SVP and JNB hold PhD fellowships PD/BD/52210/2013 and PD/BD/128214/2016, respectively, within the scope of the PhD program Molecular Biosciences (PD/00133/2012) funded by FCT.Chlamydia trachomatis is a human bacterial pathogen causing ocular and genital infections. It multiplies exclusively within an intracellular membrane-bound vacuole, the inclusion, and uses a type III secretion system to manipulate host cells by injecting them with bacteriallyencoded effector proteins. In this work, we characterized the expression and subcellular localization in infected host cells of the C. trachomatis CT142, CT143, and CT144 proteins, which we previously showed to be type III secretion substrates. Transcriptional analyses in C. trachomatis confirmed the prediction that ct142, ct143 and ct144 are organized in an operon and revealed that their expression is likely driven by the main σ factor, σ66. In host cells infected by C. trachomatis, production of CT142 and CT143 could be detected by immunoblotting from 20-26 h post-infection. Immunofluorescence microscopy of infected cells revealed that from 20 h post-infection CT143 appeared mostly as globular structures outside of the bacterial cells but within the lumen of the inclusion. Furthermore, immunofluorescence microscopy of cells infected by C. trachomatis strains carrying plasmids producing CT142, CT143, or CT144 under the control of the ct142 promoter and with a C-terminal double hemagglutinin (2HA) epitope tag revealed that CT142-2HA, CT143-2HA or CT144-2HA showed an identical localization to chromosomally-encoded CT143. Moreover, CT142-2HA or CT144-2HA and CT143 produced by the same bacteria co-localized in the lumen of the inclusion. Overall, these data suggest that the CT142, CT143, and CT144 type III secretion substrates are secreted into the lumen of the inclusion where they might form a protein complex.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaInstituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNCunha, Maria daPais, Sara V.Bugalhão, Joana N.Mota, Luís Jaime2018-05-07T22:14:27Z2017-06-012017-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1371/journal.pone.0178856eng1932-6203PURE: 3847560http://www.scopus.com/inward/record.url?scp=85020873659&partnerID=8YFLogxKhttps://doi.org/10.1371/journal.pone.0178856info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:19:36Zoai:run.unl.pt:10362/36187Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:24.317293Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion |
title |
The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion |
spellingShingle |
The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion Cunha, Maria da TRANSCRIPTIONAL REGULATOR YERSINIA-ENTEROCOLITICA TRANSLOCATED PROTEIN INC PROTEINS Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) |
title_short |
The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion |
title_full |
The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion |
title_fullStr |
The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion |
title_full_unstemmed |
The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion |
title_sort |
The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion |
author |
Cunha, Maria da |
author_facet |
Cunha, Maria da Pais, Sara V. Bugalhão, Joana N. Mota, Luís Jaime |
author_role |
author |
author2 |
Pais, Sara V. Bugalhão, Joana N. Mota, Luís Jaime |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
UCIBIO - Applied Molecular Biosciences Unit DCV - Departamento de Ciências da Vida Instituto de Tecnologia Química e Biológica António Xavier (ITQB) RUN |
dc.contributor.author.fl_str_mv |
Cunha, Maria da Pais, Sara V. Bugalhão, Joana N. Mota, Luís Jaime |
dc.subject.por.fl_str_mv |
TRANSCRIPTIONAL REGULATOR YERSINIA-ENTEROCOLITICA TRANSLOCATED PROTEIN INC PROTEINS Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) |
topic |
TRANSCRIPTIONAL REGULATOR YERSINIA-ENTEROCOLITICA TRANSLOCATED PROTEIN INC PROTEINS Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) |
description |
Fundacao para a Ciencia e a Tecnologia (FCT) through grant ERA-PTG/0005/2010 (in the frame of ERA-NET PathoGenoMics) to LJM and by Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), which is financed by national funds from FCT (UID/Multi/04378/2013) and co-financed by the European Regional Development Fund (ERDF) under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). MdC was supported by PhD fellowship SFRH/BD/62728/2009 from FCT. SVP and JNB hold PhD fellowships PD/BD/52210/2013 and PD/BD/128214/2016, respectively, within the scope of the PhD program Molecular Biosciences (PD/00133/2012) funded by FCT. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-01 2017-06-01T00:00:00Z 2018-05-07T22:14:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1371/journal.pone.0178856 |
url |
https://doi.org/10.1371/journal.pone.0178856 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1932-6203 PURE: 3847560 http://www.scopus.com/inward/record.url?scp=85020873659&partnerID=8YFLogxK https://doi.org/10.1371/journal.pone.0178856 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137928529575936 |