The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion

Detalhes bibliográficos
Autor(a) principal: Cunha, Maria da
Data de Publicação: 2017
Outros Autores: Pais, Sara V., Bugalhão, Joana N., Mota, Luís Jaime
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1371/journal.pone.0178856
Resumo: Fundacao para a Ciencia e a Tecnologia (FCT) through grant ERA-PTG/0005/2010 (in the frame of ERA-NET PathoGenoMics) to LJM and by Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), which is financed by national funds from FCT (UID/Multi/04378/2013) and co-financed by the European Regional Development Fund (ERDF) under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). MdC was supported by PhD fellowship SFRH/BD/62728/2009 from FCT. SVP and JNB hold PhD fellowships PD/BD/52210/2013 and PD/BD/128214/2016, respectively, within the scope of the PhD program Molecular Biosciences (PD/00133/2012) funded by FCT.
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spelling The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusionTRANSCRIPTIONAL REGULATORYERSINIA-ENTEROCOLITICATRANSLOCATED PROTEININC PROTEINSBiochemistry, Genetics and Molecular Biology(all)Agricultural and Biological Sciences(all)Fundacao para a Ciencia e a Tecnologia (FCT) through grant ERA-PTG/0005/2010 (in the frame of ERA-NET PathoGenoMics) to LJM and by Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), which is financed by national funds from FCT (UID/Multi/04378/2013) and co-financed by the European Regional Development Fund (ERDF) under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). MdC was supported by PhD fellowship SFRH/BD/62728/2009 from FCT. SVP and JNB hold PhD fellowships PD/BD/52210/2013 and PD/BD/128214/2016, respectively, within the scope of the PhD program Molecular Biosciences (PD/00133/2012) funded by FCT.Chlamydia trachomatis is a human bacterial pathogen causing ocular and genital infections. It multiplies exclusively within an intracellular membrane-bound vacuole, the inclusion, and uses a type III secretion system to manipulate host cells by injecting them with bacteriallyencoded effector proteins. In this work, we characterized the expression and subcellular localization in infected host cells of the C. trachomatis CT142, CT143, and CT144 proteins, which we previously showed to be type III secretion substrates. Transcriptional analyses in C. trachomatis confirmed the prediction that ct142, ct143 and ct144 are organized in an operon and revealed that their expression is likely driven by the main σ factor, σ66. In host cells infected by C. trachomatis, production of CT142 and CT143 could be detected by immunoblotting from 20-26 h post-infection. Immunofluorescence microscopy of infected cells revealed that from 20 h post-infection CT143 appeared mostly as globular structures outside of the bacterial cells but within the lumen of the inclusion. Furthermore, immunofluorescence microscopy of cells infected by C. trachomatis strains carrying plasmids producing CT142, CT143, or CT144 under the control of the ct142 promoter and with a C-terminal double hemagglutinin (2HA) epitope tag revealed that CT142-2HA, CT143-2HA or CT144-2HA showed an identical localization to chromosomally-encoded CT143. Moreover, CT142-2HA or CT144-2HA and CT143 produced by the same bacteria co-localized in the lumen of the inclusion. Overall, these data suggest that the CT142, CT143, and CT144 type III secretion substrates are secreted into the lumen of the inclusion where they might form a protein complex.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaInstituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNCunha, Maria daPais, Sara V.Bugalhão, Joana N.Mota, Luís Jaime2018-05-07T22:14:27Z2017-06-012017-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1371/journal.pone.0178856eng1932-6203PURE: 3847560http://www.scopus.com/inward/record.url?scp=85020873659&partnerID=8YFLogxKhttps://doi.org/10.1371/journal.pone.0178856info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:19:36Zoai:run.unl.pt:10362/36187Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:24.317293Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion
title The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion
spellingShingle The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion
Cunha, Maria da
TRANSCRIPTIONAL REGULATOR
YERSINIA-ENTEROCOLITICA
TRANSLOCATED PROTEIN
INC PROTEINS
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
title_short The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion
title_full The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion
title_fullStr The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion
title_full_unstemmed The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion
title_sort The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion
author Cunha, Maria da
author_facet Cunha, Maria da
Pais, Sara V.
Bugalhão, Joana N.
Mota, Luís Jaime
author_role author
author2 Pais, Sara V.
Bugalhão, Joana N.
Mota, Luís Jaime
author2_role author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Cunha, Maria da
Pais, Sara V.
Bugalhão, Joana N.
Mota, Luís Jaime
dc.subject.por.fl_str_mv TRANSCRIPTIONAL REGULATOR
YERSINIA-ENTEROCOLITICA
TRANSLOCATED PROTEIN
INC PROTEINS
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
topic TRANSCRIPTIONAL REGULATOR
YERSINIA-ENTEROCOLITICA
TRANSLOCATED PROTEIN
INC PROTEINS
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
description Fundacao para a Ciencia e a Tecnologia (FCT) through grant ERA-PTG/0005/2010 (in the frame of ERA-NET PathoGenoMics) to LJM and by Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), which is financed by national funds from FCT (UID/Multi/04378/2013) and co-financed by the European Regional Development Fund (ERDF) under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). MdC was supported by PhD fellowship SFRH/BD/62728/2009 from FCT. SVP and JNB hold PhD fellowships PD/BD/52210/2013 and PD/BD/128214/2016, respectively, within the scope of the PhD program Molecular Biosciences (PD/00133/2012) funded by FCT.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-01
2017-06-01T00:00:00Z
2018-05-07T22:14:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1371/journal.pone.0178856
url https://doi.org/10.1371/journal.pone.0178856
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1932-6203
PURE: 3847560
http://www.scopus.com/inward/record.url?scp=85020873659&partnerID=8YFLogxK
https://doi.org/10.1371/journal.pone.0178856
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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