3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/89276 |
Resumo: | Extracellular matrix (ECM)-based bioinks has attracted much attention in recent years for 3D printing of native-like tissue constructs. Due to organ unavailability, human placental ECM can be an alternative source for the construction of 3D print composite scaffolds for the treatment of deep wounds. In this study, we use different concentrations (1.5%, 3% and 5%w/v) of ECM derived from the placenta, sodium-alginate and gelatin to prepare a printable bioink biomimicking natural skin. The printed hydrogels' morphology, physical structure, mechanical behavior, biocompatibility, and angiogenic property are investigated. The optimized ECM (5%w/v) 3D printed scaffold is applied on full-thickness wounds created in a mouse model. Due to their unique native-like structure, the ECM-based scaffolds provide a non-cytotoxic microenvironment for cell adhesion, infiltration, angiogenesis, and proliferation. In contrast, they do not show any sign of immune response to the host. Notably, the biodegradation, swelling rate, mechanical property, cell adhesion and angiogenesis properties increase with the increase of ECM concentrations in the construct. The ECM 3D printed scaffold implanted into deep wounds increases granulation tissue formation, angiogenesis, and re-epithelialization due to the presence of ECM components in the construct, when compared with printed scaffold with no ECM and no treatment wound. Overall, our findings demonstrate that the 5% ECM 3D scaffold supports the best deep wound regeneration in vivo, produces a skin replacement with a cellular structure comparable to native skin. |
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3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties3D printed scaffoldAlginate/gelatinECM bioinkExtracellular matrixPlacentaWound healingExtracellular matrix (ECM)-based bioinks has attracted much attention in recent years for 3D printing of native-like tissue constructs. Due to organ unavailability, human placental ECM can be an alternative source for the construction of 3D print composite scaffolds for the treatment of deep wounds. In this study, we use different concentrations (1.5%, 3% and 5%w/v) of ECM derived from the placenta, sodium-alginate and gelatin to prepare a printable bioink biomimicking natural skin. The printed hydrogels' morphology, physical structure, mechanical behavior, biocompatibility, and angiogenic property are investigated. The optimized ECM (5%w/v) 3D printed scaffold is applied on full-thickness wounds created in a mouse model. Due to their unique native-like structure, the ECM-based scaffolds provide a non-cytotoxic microenvironment for cell adhesion, infiltration, angiogenesis, and proliferation. In contrast, they do not show any sign of immune response to the host. Notably, the biodegradation, swelling rate, mechanical property, cell adhesion and angiogenesis properties increase with the increase of ECM concentrations in the construct. The ECM 3D printed scaffold implanted into deep wounds increases granulation tissue formation, angiogenesis, and re-epithelialization due to the presence of ECM components in the construct, when compared with printed scaffold with no ECM and no treatment wound. Overall, our findings demonstrate that the 5% ECM 3D scaffold supports the best deep wound regeneration in vivo, produces a skin replacement with a cellular structure comparable to native skin.BPUMS -Bushehr University of Medical Sciences(IR.BPUMS.REC.1400.183)ElsevierUniversidade do MinhoBashiri, ZahraFomeshi, Motahareh RajabiHamidabadi, Hatef GhasemiJafari, DavodAlizadeh, SanazBojnordi, Maryam NazmOrive, GorkaDolatshahi-Pirouz, AlirezaZahiri, MariaReis, R. L.Kundu, Subhas CGholipourmalekabadi, Mazaher2023-052023-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/89276engBashiri Z., Fomeshi M. R., Hamidabadi H. G., Jafari D., Alizadeh S., Bojnordi M. N., Orive G., Dolatshahi-Pirouz A., Zahiri M., Reis R. L., Kundu S. C., Gholipourmalekabadi M. 3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties, Materials Today Bio, Vol. 20, pp. 100666, doi:10.1016/j.mtbio.2023.100666, 20232590-006410.1016/j.mtbio.2023.100666https://www.sciencedirect.com/science/article/pii/S2590006423001266info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-09T01:20:57Zoai:repositorium.sdum.uminho.pt:1822/89276Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:14:04.068033Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties |
title |
3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties |
spellingShingle |
3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties Bashiri, Zahra 3D printed scaffold Alginate/gelatin ECM bioink Extracellular matrix Placenta Wound healing |
title_short |
3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties |
title_full |
3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties |
title_fullStr |
3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties |
title_full_unstemmed |
3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties |
title_sort |
3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties |
author |
Bashiri, Zahra |
author_facet |
Bashiri, Zahra Fomeshi, Motahareh Rajabi Hamidabadi, Hatef Ghasemi Jafari, Davod Alizadeh, Sanaz Bojnordi, Maryam Nazm Orive, Gorka Dolatshahi-Pirouz, Alireza Zahiri, Maria Reis, R. L. Kundu, Subhas C Gholipourmalekabadi, Mazaher |
author_role |
author |
author2 |
Fomeshi, Motahareh Rajabi Hamidabadi, Hatef Ghasemi Jafari, Davod Alizadeh, Sanaz Bojnordi, Maryam Nazm Orive, Gorka Dolatshahi-Pirouz, Alireza Zahiri, Maria Reis, R. L. Kundu, Subhas C Gholipourmalekabadi, Mazaher |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Bashiri, Zahra Fomeshi, Motahareh Rajabi Hamidabadi, Hatef Ghasemi Jafari, Davod Alizadeh, Sanaz Bojnordi, Maryam Nazm Orive, Gorka Dolatshahi-Pirouz, Alireza Zahiri, Maria Reis, R. L. Kundu, Subhas C Gholipourmalekabadi, Mazaher |
dc.subject.por.fl_str_mv |
3D printed scaffold Alginate/gelatin ECM bioink Extracellular matrix Placenta Wound healing |
topic |
3D printed scaffold Alginate/gelatin ECM bioink Extracellular matrix Placenta Wound healing |
description |
Extracellular matrix (ECM)-based bioinks has attracted much attention in recent years for 3D printing of native-like tissue constructs. Due to organ unavailability, human placental ECM can be an alternative source for the construction of 3D print composite scaffolds for the treatment of deep wounds. In this study, we use different concentrations (1.5%, 3% and 5%w/v) of ECM derived from the placenta, sodium-alginate and gelatin to prepare a printable bioink biomimicking natural skin. The printed hydrogels' morphology, physical structure, mechanical behavior, biocompatibility, and angiogenic property are investigated. The optimized ECM (5%w/v) 3D printed scaffold is applied on full-thickness wounds created in a mouse model. Due to their unique native-like structure, the ECM-based scaffolds provide a non-cytotoxic microenvironment for cell adhesion, infiltration, angiogenesis, and proliferation. In contrast, they do not show any sign of immune response to the host. Notably, the biodegradation, swelling rate, mechanical property, cell adhesion and angiogenesis properties increase with the increase of ECM concentrations in the construct. The ECM 3D printed scaffold implanted into deep wounds increases granulation tissue formation, angiogenesis, and re-epithelialization due to the presence of ECM components in the construct, when compared with printed scaffold with no ECM and no treatment wound. Overall, our findings demonstrate that the 5% ECM 3D scaffold supports the best deep wound regeneration in vivo, produces a skin replacement with a cellular structure comparable to native skin. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05 2023-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/89276 |
url |
https://hdl.handle.net/1822/89276 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bashiri Z., Fomeshi M. R., Hamidabadi H. G., Jafari D., Alizadeh S., Bojnordi M. N., Orive G., Dolatshahi-Pirouz A., Zahiri M., Reis R. L., Kundu S. C., Gholipourmalekabadi M. 3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties, Materials Today Bio, Vol. 20, pp. 100666, doi:10.1016/j.mtbio.2023.100666, 2023 2590-0064 10.1016/j.mtbio.2023.100666 https://www.sciencedirect.com/science/article/pii/S2590006423001266 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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