Cherry extract from prunus avium L. To improve the resistance of endothelial cells to oxidative stress: Mucoadhesive chitosan vs. poly(lactic-co-glycolic acid) nanoparticles

Detalhes bibliográficos
Autor(a) principal: Beconcini, D
Data de Publicação: 2019
Outros Autores: Fabiano, A, Stefano, R, Macedo, MH, Felice, F, Zambito, Y, Sarmento, B
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/136322
Resumo: Polyphenolic compounds contained in cherry extract (CE) are well known for their antioxidant and anti-inflammatory properties. Unfortunately, most of these natural compounds have low oral bioavailability, reducing their widespread use. Here, different concentrations of polyphenol-rich CE from Tuscany (Italy), encapsulated in poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs), were compared with those encapsulated in two NP types, different from each other in terms of mucoadhesivity, obtained with chitosan derivatives (Ch-der), regarding CE gastrointestinal (GI) permeability and protective effect on oxidative stress. Different NP systems were physico-chemically characterized, and the antioxidant GI permeability was evaluated in a triple-cell co-culture model (Caco-2/HT29-MTX/Raji B), resembling the intestine. PLGA NPs efficiently entrapped CE (up to 840 µg gallic acid equivalent (GAE)/mL) without altering size (210 nm), polydispersity index (0.05), or zeta potential (-10.7 mV). Such NPs promoted permeation of encapsulated CE at a CE polyphenolic concentration of at least 2 µg GAE/mL. More mucoadhesive NPs from Ch-der, coded quaternary ammonium S-protected thiolated chitosan (QA-Ch-S-pro) NP, promoted CE GI permeation of 0.5 µg GAE/mL. At higher concentrations of Ch-der polymers, the resulting NPs containing CE were toxic toward Caco-2 and HT29-MTX cells. CE protected human umbilical vein endothelial cells (HUVECs) from oxidative stress and maintained its activity when entrapped in PLGA NPs. CE encapsulated in QA-Ch-S-pro NP protected HUVECs from oxidative stress, even more effectively than non-encapsulated CE. Furthermore, mucoadhesive NPs from Ch-der were more effective antioxidant protectors than PLGA NPs, but less cytotoxic PLGA NPs could be more useful when comparatively high therapeutic antioxidant doses are needed.
id RCAP_a7e8fd1170b19531960d6fc822056bf3
oai_identifier_str oai:repositorio-aberto.up.pt:10216/136322
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Cherry extract from prunus avium L. To improve the resistance of endothelial cells to oxidative stress: Mucoadhesive chitosan vs. poly(lactic-co-glycolic acid) nanoparticlesChitosan nanoparticlesHUVECsIntestinal permeabilityOxidative stressPLGA nanoparticlesPolyphenolsSweet cherry (Prunus avium L.)Polyphenolic compounds contained in cherry extract (CE) are well known for their antioxidant and anti-inflammatory properties. Unfortunately, most of these natural compounds have low oral bioavailability, reducing their widespread use. Here, different concentrations of polyphenol-rich CE from Tuscany (Italy), encapsulated in poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs), were compared with those encapsulated in two NP types, different from each other in terms of mucoadhesivity, obtained with chitosan derivatives (Ch-der), regarding CE gastrointestinal (GI) permeability and protective effect on oxidative stress. Different NP systems were physico-chemically characterized, and the antioxidant GI permeability was evaluated in a triple-cell co-culture model (Caco-2/HT29-MTX/Raji B), resembling the intestine. PLGA NPs efficiently entrapped CE (up to 840 µg gallic acid equivalent (GAE)/mL) without altering size (210 nm), polydispersity index (0.05), or zeta potential (-10.7 mV). Such NPs promoted permeation of encapsulated CE at a CE polyphenolic concentration of at least 2 µg GAE/mL. More mucoadhesive NPs from Ch-der, coded quaternary ammonium S-protected thiolated chitosan (QA-Ch-S-pro) NP, promoted CE GI permeation of 0.5 µg GAE/mL. At higher concentrations of Ch-der polymers, the resulting NPs containing CE were toxic toward Caco-2 and HT29-MTX cells. CE protected human umbilical vein endothelial cells (HUVECs) from oxidative stress and maintained its activity when entrapped in PLGA NPs. CE encapsulated in QA-Ch-S-pro NP protected HUVECs from oxidative stress, even more effectively than non-encapsulated CE. Furthermore, mucoadhesive NPs from Ch-der were more effective antioxidant protectors than PLGA NPs, but less cytotoxic PLGA NPs could be more useful when comparatively high therapeutic antioxidant doses are needed.MDPI20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/136322eng1661-659610.3390/ijms20071759Beconcini, DFabiano, AStefano, RMacedo, MHFelice, FZambito, YSarmento, Binfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T15:51:25Zoai:repositorio-aberto.up.pt:10216/136322Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:33:50.452153Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cherry extract from prunus avium L. To improve the resistance of endothelial cells to oxidative stress: Mucoadhesive chitosan vs. poly(lactic-co-glycolic acid) nanoparticles
title Cherry extract from prunus avium L. To improve the resistance of endothelial cells to oxidative stress: Mucoadhesive chitosan vs. poly(lactic-co-glycolic acid) nanoparticles
spellingShingle Cherry extract from prunus avium L. To improve the resistance of endothelial cells to oxidative stress: Mucoadhesive chitosan vs. poly(lactic-co-glycolic acid) nanoparticles
Beconcini, D
Chitosan nanoparticles
HUVECs
Intestinal permeability
Oxidative stress
PLGA nanoparticles
Polyphenols
Sweet cherry (Prunus avium L.)
title_short Cherry extract from prunus avium L. To improve the resistance of endothelial cells to oxidative stress: Mucoadhesive chitosan vs. poly(lactic-co-glycolic acid) nanoparticles
title_full Cherry extract from prunus avium L. To improve the resistance of endothelial cells to oxidative stress: Mucoadhesive chitosan vs. poly(lactic-co-glycolic acid) nanoparticles
title_fullStr Cherry extract from prunus avium L. To improve the resistance of endothelial cells to oxidative stress: Mucoadhesive chitosan vs. poly(lactic-co-glycolic acid) nanoparticles
title_full_unstemmed Cherry extract from prunus avium L. To improve the resistance of endothelial cells to oxidative stress: Mucoadhesive chitosan vs. poly(lactic-co-glycolic acid) nanoparticles
title_sort Cherry extract from prunus avium L. To improve the resistance of endothelial cells to oxidative stress: Mucoadhesive chitosan vs. poly(lactic-co-glycolic acid) nanoparticles
author Beconcini, D
author_facet Beconcini, D
Fabiano, A
Stefano, R
Macedo, MH
Felice, F
Zambito, Y
Sarmento, B
author_role author
author2 Fabiano, A
Stefano, R
Macedo, MH
Felice, F
Zambito, Y
Sarmento, B
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Beconcini, D
Fabiano, A
Stefano, R
Macedo, MH
Felice, F
Zambito, Y
Sarmento, B
dc.subject.por.fl_str_mv Chitosan nanoparticles
HUVECs
Intestinal permeability
Oxidative stress
PLGA nanoparticles
Polyphenols
Sweet cherry (Prunus avium L.)
topic Chitosan nanoparticles
HUVECs
Intestinal permeability
Oxidative stress
PLGA nanoparticles
Polyphenols
Sweet cherry (Prunus avium L.)
description Polyphenolic compounds contained in cherry extract (CE) are well known for their antioxidant and anti-inflammatory properties. Unfortunately, most of these natural compounds have low oral bioavailability, reducing their widespread use. Here, different concentrations of polyphenol-rich CE from Tuscany (Italy), encapsulated in poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs), were compared with those encapsulated in two NP types, different from each other in terms of mucoadhesivity, obtained with chitosan derivatives (Ch-der), regarding CE gastrointestinal (GI) permeability and protective effect on oxidative stress. Different NP systems were physico-chemically characterized, and the antioxidant GI permeability was evaluated in a triple-cell co-culture model (Caco-2/HT29-MTX/Raji B), resembling the intestine. PLGA NPs efficiently entrapped CE (up to 840 µg gallic acid equivalent (GAE)/mL) without altering size (210 nm), polydispersity index (0.05), or zeta potential (-10.7 mV). Such NPs promoted permeation of encapsulated CE at a CE polyphenolic concentration of at least 2 µg GAE/mL. More mucoadhesive NPs from Ch-der, coded quaternary ammonium S-protected thiolated chitosan (QA-Ch-S-pro) NP, promoted CE GI permeation of 0.5 µg GAE/mL. At higher concentrations of Ch-der polymers, the resulting NPs containing CE were toxic toward Caco-2 and HT29-MTX cells. CE protected human umbilical vein endothelial cells (HUVECs) from oxidative stress and maintained its activity when entrapped in PLGA NPs. CE encapsulated in QA-Ch-S-pro NP protected HUVECs from oxidative stress, even more effectively than non-encapsulated CE. Furthermore, mucoadhesive NPs from Ch-der were more effective antioxidant protectors than PLGA NPs, but less cytotoxic PLGA NPs could be more useful when comparatively high therapeutic antioxidant doses are needed.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/136322
url https://hdl.handle.net/10216/136322
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1661-6596
10.3390/ijms20071759
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799136247418978304