Discovery and characterization of auxiliary proteins encoded by type 3 simian T-cell lymphotropic viruses

Detalhes bibliográficos
Autor(a) principal: Turpin, Jocelyn
Data de Publicação: 2015
Outros Autores: Journo, Chloé, Ko, Nga Ling, Sinet, Flore, Carpentier, Alexandre, Galioot, Amandine, Edwards, Dustin, Vandamme, Anne Mieke, Gazzolo, Louis, Dodon, Madeleine Duc, Gessain, Antoine, Kashanchi, Fatah, Balansard, Ivan, Lacoste, Romain, Mahieux, Renaud
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1128/JVI.02150-14
Resumo: WOS:000347178900005 PMID:25355890
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spelling Discovery and characterization of auxiliary proteins encoded by type 3 simian T-cell lymphotropic virusesImmunologyVirologyMedicine(all)WOS:000347178900005 PMID:25355890Human T-cell lymphotropic virus type 1 (HTLV-1) and HTLV-2 encode auxiliary proteins that play important roles in viral replication, viral latency, and immune escape. The presence of auxiliary protein-encoding open reading frames (ORFs) in HTLV-3, the latest HTLV to be discovered, is unknown. Simian T-cell lymphotropic virus type 3 (STLV-3) is almost identical to HTLV-3. Given the lack of HTLV-3-infected cell lines, we took advantage of STLV-3-infected cells and of an STLV-3 molecular clone to search for the presence of auxiliary transcripts. Using reverse transcriptase PCR (RT-PCR), we first uncovered the presence of three unknown viral mRNAs encoding putative proteins of 5, 8, and 9 kDa and confirmed the presence of the previously reported RorfII transcript. The existence of these viral mRNAs was confirmed by using splice site-specific RT-PCR with ex vivo samples.Weshowed that p5 is distributed throughout the cell and does not colocalize with a specific organelle. The p9 localization is similar to that of HTLV-1 p12 and induced a strong decrease in the calreticulin signal, similarly to HTLV-1 p12. Although p8, RorfII, and Rex-3 share an N-terminal sequence that is predicted to contain a nucleolar localization signal (NoLS), only p8 is found in the nucleolus. The p8 location in the nucleolus is linked to a bipartite NoLS. p8 and, to a lesser extent, p9 repressed viral expression but did not alter Rex-3-dependent mRNA export. Using a transformation assay, we finally showed that none of the STLV-3 auxiliary proteins had the ability to induce colony formation, while both Tax-3 and antisense protein of HTLV-3 (APH-3) promoted cellular transformation. Altogether, these results complete the characterization of the newly described primate T-lymphotropic virus type 3 (PTLV-3).Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)TB, HIV and opportunistic diseases and pathogens (THOP)RUNTurpin, JocelynJourno, ChloéKo, Nga LingSinet, FloreCarpentier, AlexandreGalioot, AmandineEdwards, DustinVandamme, Anne MiekeGazzolo, LouisDodon, Madeleine DucGessain, AntoineKashanchi, FatahBalansard, IvanLacoste, RomainMahieux, Renaud2018-05-14T22:02:38Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article21application/pdfhttps://doi.org/10.1128/JVI.02150-14eng0022-538XPURE: 1732973http://www.scopus.com/inward/record.url?scp=84920825212&partnerID=8YFLogxKhttps://doi.org/10.1128/JVI.02150-14info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:20:31Zoai:run.unl.pt:10362/36950Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:43.592983Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Discovery and characterization of auxiliary proteins encoded by type 3 simian T-cell lymphotropic viruses
title Discovery and characterization of auxiliary proteins encoded by type 3 simian T-cell lymphotropic viruses
spellingShingle Discovery and characterization of auxiliary proteins encoded by type 3 simian T-cell lymphotropic viruses
Turpin, Jocelyn
Immunology
Virology
Medicine(all)
title_short Discovery and characterization of auxiliary proteins encoded by type 3 simian T-cell lymphotropic viruses
title_full Discovery and characterization of auxiliary proteins encoded by type 3 simian T-cell lymphotropic viruses
title_fullStr Discovery and characterization of auxiliary proteins encoded by type 3 simian T-cell lymphotropic viruses
title_full_unstemmed Discovery and characterization of auxiliary proteins encoded by type 3 simian T-cell lymphotropic viruses
title_sort Discovery and characterization of auxiliary proteins encoded by type 3 simian T-cell lymphotropic viruses
author Turpin, Jocelyn
author_facet Turpin, Jocelyn
Journo, Chloé
Ko, Nga Ling
Sinet, Flore
Carpentier, Alexandre
Galioot, Amandine
Edwards, Dustin
Vandamme, Anne Mieke
Gazzolo, Louis
Dodon, Madeleine Duc
Gessain, Antoine
Kashanchi, Fatah
Balansard, Ivan
Lacoste, Romain
Mahieux, Renaud
author_role author
author2 Journo, Chloé
Ko, Nga Ling
Sinet, Flore
Carpentier, Alexandre
Galioot, Amandine
Edwards, Dustin
Vandamme, Anne Mieke
Gazzolo, Louis
Dodon, Madeleine Duc
Gessain, Antoine
Kashanchi, Fatah
Balansard, Ivan
Lacoste, Romain
Mahieux, Renaud
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
Global Health and Tropical Medicine (GHTM)
TB, HIV and opportunistic diseases and pathogens (THOP)
RUN
dc.contributor.author.fl_str_mv Turpin, Jocelyn
Journo, Chloé
Ko, Nga Ling
Sinet, Flore
Carpentier, Alexandre
Galioot, Amandine
Edwards, Dustin
Vandamme, Anne Mieke
Gazzolo, Louis
Dodon, Madeleine Duc
Gessain, Antoine
Kashanchi, Fatah
Balansard, Ivan
Lacoste, Romain
Mahieux, Renaud
dc.subject.por.fl_str_mv Immunology
Virology
Medicine(all)
topic Immunology
Virology
Medicine(all)
description WOS:000347178900005 PMID:25355890
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2018-05-14T22:02:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1128/JVI.02150-14
url https://doi.org/10.1128/JVI.02150-14
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0022-538X
PURE: 1732973
http://www.scopus.com/inward/record.url?scp=84920825212&partnerID=8YFLogxK
https://doi.org/10.1128/JVI.02150-14
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 21
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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