Methods to Study Centrosomes and Cilia in Drosophila

Detalhes bibliográficos
Autor(a) principal: Jana, Swadhin Chandra
Data de Publicação: 2016
Outros Autores: Mendonça, Susana, Werner, Sascha, Bettencourt-Dias, Monica
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.7/832
Resumo: Centrioles and cilia are highly conserved eukaryotic organelles. Drosophila melanogaster is a powerful genetic and cell biology model organism, extensively used to discover underlying mechanisms of centrosome and cilia biogenesis and function. Defects in centrosomes and cilia reduce fertility and affect different sensory functions, such as proprioception, olfaction, and hearing. The fly possesses a large diversity of ciliary structures and assembly modes, such as motile, immotile, and intraflagellar transport (IFT)-independent or IFT-dependent assembly. Moreover, all the diverse ciliated cells harbor centrioles at the base of the cilia, called basal bodies, making the fly an attractive model to better understand the biology of this organelle. This chapter describes protocols to visualize centrosomes and cilia by fluorescence and electron microscopy.
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spelling Methods to Study Centrosomes and Cilia in DrosophilaDrosophilaCentrosomeBasal bodyCiliaIntraflagellar transportSensory neuronSpermCentrioles and cilia are highly conserved eukaryotic organelles. Drosophila melanogaster is a powerful genetic and cell biology model organism, extensively used to discover underlying mechanisms of centrosome and cilia biogenesis and function. Defects in centrosomes and cilia reduce fertility and affect different sensory functions, such as proprioception, olfaction, and hearing. The fly possesses a large diversity of ciliary structures and assembly modes, such as motile, immotile, and intraflagellar transport (IFT)-independent or IFT-dependent assembly. Moreover, all the diverse ciliated cells harbor centrioles at the base of the cilia, called basal bodies, making the fly an attractive model to better understand the biology of this organelle. This chapter describes protocols to visualize centrosomes and cilia by fluorescence and electron microscopy.Humana PressARCAJana, Swadhin ChandraMendonça, SusanaWerner, SaschaBettencourt-Dias, Monica2018-02-07T17:16:59Z2016-08-122016-08-12T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/832eng10.1007/978-1-4939-3789-9_14info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-21T14:21:09Zoai:arca.igc.gulbenkian.pt:10400.7/832Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-21T14:21:09Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Methods to Study Centrosomes and Cilia in Drosophila
title Methods to Study Centrosomes and Cilia in Drosophila
spellingShingle Methods to Study Centrosomes and Cilia in Drosophila
Jana, Swadhin Chandra
Drosophila
Centrosome
Basal body
Cilia
Intraflagellar transport
Sensory neuron
Sperm
title_short Methods to Study Centrosomes and Cilia in Drosophila
title_full Methods to Study Centrosomes and Cilia in Drosophila
title_fullStr Methods to Study Centrosomes and Cilia in Drosophila
title_full_unstemmed Methods to Study Centrosomes and Cilia in Drosophila
title_sort Methods to Study Centrosomes and Cilia in Drosophila
author Jana, Swadhin Chandra
author_facet Jana, Swadhin Chandra
Mendonça, Susana
Werner, Sascha
Bettencourt-Dias, Monica
author_role author
author2 Mendonça, Susana
Werner, Sascha
Bettencourt-Dias, Monica
author2_role author
author
author
dc.contributor.none.fl_str_mv ARCA
dc.contributor.author.fl_str_mv Jana, Swadhin Chandra
Mendonça, Susana
Werner, Sascha
Bettencourt-Dias, Monica
dc.subject.por.fl_str_mv Drosophila
Centrosome
Basal body
Cilia
Intraflagellar transport
Sensory neuron
Sperm
topic Drosophila
Centrosome
Basal body
Cilia
Intraflagellar transport
Sensory neuron
Sperm
description Centrioles and cilia are highly conserved eukaryotic organelles. Drosophila melanogaster is a powerful genetic and cell biology model organism, extensively used to discover underlying mechanisms of centrosome and cilia biogenesis and function. Defects in centrosomes and cilia reduce fertility and affect different sensory functions, such as proprioception, olfaction, and hearing. The fly possesses a large diversity of ciliary structures and assembly modes, such as motile, immotile, and intraflagellar transport (IFT)-independent or IFT-dependent assembly. Moreover, all the diverse ciliated cells harbor centrioles at the base of the cilia, called basal bodies, making the fly an attractive model to better understand the biology of this organelle. This chapter describes protocols to visualize centrosomes and cilia by fluorescence and electron microscopy.
publishDate 2016
dc.date.none.fl_str_mv 2016-08-12
2016-08-12T00:00:00Z
2018-02-07T17:16:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.7/832
url http://hdl.handle.net/10400.7/832
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1007/978-1-4939-3789-9_14
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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