The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.21/16386 |
Resumo: | Centrosomes are organelles consisting of two structurally and functionally distinct centrioles, with the mother centriole having complex distal (DA) and subdistal appendages (SDA). Despite their importance, how appendages are assembled and maintained remains unclear. This study investigated human TBCCD1, a centrosomal protein essential for centrosome positioning, to uncover its localization and role at centrioles. We found that TBCCD1 localizes at both proximal and distal regions of the two centrioles, forming a complex structure spanning from SDA to DA and extending inside and outside the centriole lumen. TBCCD1 depletion caused centrosome mispositioning, which was partially rescued by taxol, and the loss of microtubules (MTs) anchored to centrosomes. TBCCD1 depletion also reduced levels of SDA proteins involved in MT anchoring such as Centriolin/CEP110, Ninein, and CEP170. Additionally, TBCCD1 was essential for the correct positioning of motile cilia basal bodies and associated structures in Paramecium. This study reveals that TBCCD1 is an evolutionarily conserved protein essential for centriole and basal body localization and appendage assembly and maintenance. A BioID screening also linked TBCCD1 to ciliopathy-associated protein networks. |
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The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1TBCCD1CentrosomeCentriole appendagesCiliaBasal bodyMicrotubulesParameciumCentrosomes are organelles consisting of two structurally and functionally distinct centrioles, with the mother centriole having complex distal (DA) and subdistal appendages (SDA). Despite their importance, how appendages are assembled and maintained remains unclear. This study investigated human TBCCD1, a centrosomal protein essential for centrosome positioning, to uncover its localization and role at centrioles. We found that TBCCD1 localizes at both proximal and distal regions of the two centrioles, forming a complex structure spanning from SDA to DA and extending inside and outside the centriole lumen. TBCCD1 depletion caused centrosome mispositioning, which was partially rescued by taxol, and the loss of microtubules (MTs) anchored to centrosomes. TBCCD1 depletion also reduced levels of SDA proteins involved in MT anchoring such as Centriolin/CEP110, Ninein, and CEP170. Additionally, TBCCD1 was essential for the correct positioning of motile cilia basal bodies and associated structures in Paramecium. This study reveals that TBCCD1 is an evolutionarily conserved protein essential for centriole and basal body localization and appendage assembly and maintenance. A BioID screening also linked TBCCD1 to ciliopathy-associated protein networks.RCIPLCarmona, BrunoCamelo, CarolinaMehraz, ManonLemullois, MichelFaria, Mariana LinceCoyaud, ÉtienneMarinho, H. SusanaGonçalves, JoãoNolasco, SofiaPinto, FranciscoRaught, BrianTassin, Anne-MarieKoll, FranceSoares, Helena2023-08-22T13:52:43Z2023-072023-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/16386engCarmona B, Camelo C, Mehraz M, Lemullois M, Nolasco S, Soares H, et al. The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1. BioRxiv [preprint]. 2023 July 26.10.1101/2023.07.26.549647info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-23T02:15:36Zoai:repositorio.ipl.pt:10400.21/16386Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:27:24.665493Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1 |
title |
The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1 |
spellingShingle |
The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1 Carmona, Bruno TBCCD1 Centrosome Centriole appendages Cilia Basal body Microtubules Paramecium |
title_short |
The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1 |
title_full |
The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1 |
title_fullStr |
The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1 |
title_full_unstemmed |
The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1 |
title_sort |
The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1 |
author |
Carmona, Bruno |
author_facet |
Carmona, Bruno Camelo, Carolina Mehraz, Manon Lemullois, Michel Faria, Mariana Lince Coyaud, Étienne Marinho, H. Susana Gonçalves, João Nolasco, Sofia Pinto, Francisco Raught, Brian Tassin, Anne-Marie Koll, France Soares, Helena |
author_role |
author |
author2 |
Camelo, Carolina Mehraz, Manon Lemullois, Michel Faria, Mariana Lince Coyaud, Étienne Marinho, H. Susana Gonçalves, João Nolasco, Sofia Pinto, Francisco Raught, Brian Tassin, Anne-Marie Koll, France Soares, Helena |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
RCIPL |
dc.contributor.author.fl_str_mv |
Carmona, Bruno Camelo, Carolina Mehraz, Manon Lemullois, Michel Faria, Mariana Lince Coyaud, Étienne Marinho, H. Susana Gonçalves, João Nolasco, Sofia Pinto, Francisco Raught, Brian Tassin, Anne-Marie Koll, France Soares, Helena |
dc.subject.por.fl_str_mv |
TBCCD1 Centrosome Centriole appendages Cilia Basal body Microtubules Paramecium |
topic |
TBCCD1 Centrosome Centriole appendages Cilia Basal body Microtubules Paramecium |
description |
Centrosomes are organelles consisting of two structurally and functionally distinct centrioles, with the mother centriole having complex distal (DA) and subdistal appendages (SDA). Despite their importance, how appendages are assembled and maintained remains unclear. This study investigated human TBCCD1, a centrosomal protein essential for centrosome positioning, to uncover its localization and role at centrioles. We found that TBCCD1 localizes at both proximal and distal regions of the two centrioles, forming a complex structure spanning from SDA to DA and extending inside and outside the centriole lumen. TBCCD1 depletion caused centrosome mispositioning, which was partially rescued by taxol, and the loss of microtubules (MTs) anchored to centrosomes. TBCCD1 depletion also reduced levels of SDA proteins involved in MT anchoring such as Centriolin/CEP110, Ninein, and CEP170. Additionally, TBCCD1 was essential for the correct positioning of motile cilia basal bodies and associated structures in Paramecium. This study reveals that TBCCD1 is an evolutionarily conserved protein essential for centriole and basal body localization and appendage assembly and maintenance. A BioID screening also linked TBCCD1 to ciliopathy-associated protein networks. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-08-22T13:52:43Z 2023-07 2023-07-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.21/16386 |
url |
http://hdl.handle.net/10400.21/16386 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Carmona B, Camelo C, Mehraz M, Lemullois M, Nolasco S, Soares H, et al. The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1. BioRxiv [preprint]. 2023 July 26. 10.1101/2023.07.26.549647 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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