Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation
| Autor(a) principal: | |
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| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/46298 |
Resumo: | © Author(s) (or their employer(s)) 2020. Re- use permitted under CC BY- NC. No commercial re- use. See rights and permissions. Published by BMJ. |
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Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammationBiomarkersCellularHead and neck neoplasmsImmunityImmunoassayMyeloid-derived suppressor cellsTumor© Author(s) (or their employer(s)) 2020. Re- use permitted under CC BY- NC. No commercial re- use. See rights and permissions. Published by BMJ.Background: Myeloid-derived suppressor cells (MDSC) are a functional myeloid cell subset that includes myeloid cells with immune suppressive properties. The presence of MDSC has been reported in the peripheral blood of patients with several malignant and non-malignant diseases. So far, direct comparison of MDSC across different diseases and Centers is hindered by technical pitfalls and a lack of standardized methodology. To overcome this issue, we formed a network through the COST Action Mye-EUNITER (www.mye-euniter.eu) with the goal to standardize and facilitate the comparative analysis of human circulating MDSC in cancer, inflammation and infection. In this manuscript, we present the results of the multicenter study Mye-EUNITER MDSC Monitoring Initiative, that involved 13 laboratories and compared circulating MDSC subsets across multiple diseases, using a common protocol for the isolation, identification and characterization of these cells. Methods: We developed, tested, executed and optimized a standard operating procedure for the isolation and immunophenotyping of MDSC using blood from healthy donors. We applied this procedure to the blood of almost 400 patients and controls with different solid tumors and non-malignant diseases. The latter included viral infections such as HIV and hepatitis B virus, but also psoriasis and cardiovascular disorders. Results: We observed that the frequency of MDSC in healthy donors varied substantially between centers and was influenced by technical aspects such as the anticoagulant and separation method used. Expansion of polymorphonuclear (PMN)-MDSC exceeded the expansion of monocytic MDSC (M-MDSC) in five out of six solid tumors. PMN-MDSC expansion was more pronounced in cancer compared with infection and inflammation. Programmed death-ligand 1 was primarily expressed in M-MDSC and e-MDSC and was not upregulated as a consequence of disease. LOX-1 expression was confined to PMN-MDSC. Conclusions: This study provides improved technical protocols and workflows for the multi-center analysis of circulating human MDSC subsets. Application of these workflows revealed a predominant expansion of PMN-MDSC in solid tumors that exceeds expansion in chronic infection and inflammation.Lab10: This work was funded by the following grants: PTDC/MED- IMU/30474/2017 – project cofunded by FEDER LISBOA-01–0145- FEDER-030474, through Programa Operacional Regional de Lisboa, do PORTUGAL 2020, and Fundação para a Ciência e a Tecnologia and by Gilead Genesis to AES.BMJ Publishing Group Ltd.Repositório da Universidade de LisboaCassetta, LucaBruderek, KirstenSkrzeczynska-Moncznik, JoannaOsiecka, OktawiaHu, XiaoyingRundgren, Ida MarieLin, AngSantegoets, KimHorzum, UtkuGodinho-Santos, AnaZelinskyy, GennadiyGarcia-Tellez, ThaliaBjelica, SunčicaTaciak, BartłomiejKittang, Astrid OlsnesHöing, BenediktLang, StephanDixon, MichaelMüller, VerenaUtikal, Jochen SvenKarakoç, DeryaYilmaz, Kerim BoraGórka, EmiliaBodnar, LubomirAnastasiou, Olympia EvdoxiaBourgeois, ChristineBadura, RobertKapinska-Mrowiecka, MonikaGotic, MirjanaTer Laan, MarkKers-Rebel, EstherKról, MagdalenaSantibañez, Juan FranciscoMüller-Trutwin, MichaelaDittmer, UlfSousa, Ana E.Esendağlı, GüneşAdema, GosseLoré, KarinErsvær, ElisabethUmansky, ViktorPollard, Jeffrey W.Cichy, JoannaBrandau, Sven2021-02-12T12:04:18Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/46298engJ Immunother Cancer. 2020 Sep;8(2):e00122310.1136/jitc-2020-0012232051-1426info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:48:33Zoai:repositorio.ul.pt:10451/46298Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:58:32.067672Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation |
| title |
Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation |
| spellingShingle |
Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation Cassetta, Luca Biomarkers Cellular Head and neck neoplasms Immunity Immunoassay Myeloid-derived suppressor cells Tumor |
| title_short |
Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation |
| title_full |
Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation |
| title_fullStr |
Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation |
| title_full_unstemmed |
Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation |
| title_sort |
Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation |
| author |
Cassetta, Luca |
| author_facet |
Cassetta, Luca Bruderek, Kirsten Skrzeczynska-Moncznik, Joanna Osiecka, Oktawia Hu, Xiaoying Rundgren, Ida Marie Lin, Ang Santegoets, Kim Horzum, Utku Godinho-Santos, Ana Zelinskyy, Gennadiy Garcia-Tellez, Thalia Bjelica, Sunčica Taciak, Bartłomiej Kittang, Astrid Olsnes Höing, Benedikt Lang, Stephan Dixon, Michael Müller, Verena Utikal, Jochen Sven Karakoç, Derya Yilmaz, Kerim Bora Górka, Emilia Bodnar, Lubomir Anastasiou, Olympia Evdoxia Bourgeois, Christine Badura, Robert Kapinska-Mrowiecka, Monika Gotic, Mirjana Ter Laan, Mark Kers-Rebel, Esther Król, Magdalena Santibañez, Juan Francisco Müller-Trutwin, Michaela Dittmer, Ulf Sousa, Ana E. Esendağlı, Güneş Adema, Gosse Loré, Karin Ersvær, Elisabeth Umansky, Viktor Pollard, Jeffrey W. Cichy, Joanna Brandau, Sven |
| author_role |
author |
| author2 |
Bruderek, Kirsten Skrzeczynska-Moncznik, Joanna Osiecka, Oktawia Hu, Xiaoying Rundgren, Ida Marie Lin, Ang Santegoets, Kim Horzum, Utku Godinho-Santos, Ana Zelinskyy, Gennadiy Garcia-Tellez, Thalia Bjelica, Sunčica Taciak, Bartłomiej Kittang, Astrid Olsnes Höing, Benedikt Lang, Stephan Dixon, Michael Müller, Verena Utikal, Jochen Sven Karakoç, Derya Yilmaz, Kerim Bora Górka, Emilia Bodnar, Lubomir Anastasiou, Olympia Evdoxia Bourgeois, Christine Badura, Robert Kapinska-Mrowiecka, Monika Gotic, Mirjana Ter Laan, Mark Kers-Rebel, Esther Król, Magdalena Santibañez, Juan Francisco Müller-Trutwin, Michaela Dittmer, Ulf Sousa, Ana E. Esendağlı, Güneş Adema, Gosse Loré, Karin Ersvær, Elisabeth Umansky, Viktor Pollard, Jeffrey W. Cichy, Joanna Brandau, Sven |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Cassetta, Luca Bruderek, Kirsten Skrzeczynska-Moncznik, Joanna Osiecka, Oktawia Hu, Xiaoying Rundgren, Ida Marie Lin, Ang Santegoets, Kim Horzum, Utku Godinho-Santos, Ana Zelinskyy, Gennadiy Garcia-Tellez, Thalia Bjelica, Sunčica Taciak, Bartłomiej Kittang, Astrid Olsnes Höing, Benedikt Lang, Stephan Dixon, Michael Müller, Verena Utikal, Jochen Sven Karakoç, Derya Yilmaz, Kerim Bora Górka, Emilia Bodnar, Lubomir Anastasiou, Olympia Evdoxia Bourgeois, Christine Badura, Robert Kapinska-Mrowiecka, Monika Gotic, Mirjana Ter Laan, Mark Kers-Rebel, Esther Król, Magdalena Santibañez, Juan Francisco Müller-Trutwin, Michaela Dittmer, Ulf Sousa, Ana E. Esendağlı, Güneş Adema, Gosse Loré, Karin Ersvær, Elisabeth Umansky, Viktor Pollard, Jeffrey W. Cichy, Joanna Brandau, Sven |
| dc.subject.por.fl_str_mv |
Biomarkers Cellular Head and neck neoplasms Immunity Immunoassay Myeloid-derived suppressor cells Tumor |
| topic |
Biomarkers Cellular Head and neck neoplasms Immunity Immunoassay Myeloid-derived suppressor cells Tumor |
| description |
© Author(s) (or their employer(s)) 2020. Re- use permitted under CC BY- NC. No commercial re- use. See rights and permissions. Published by BMJ. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z 2021-02-12T12:04:18Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10451/46298 |
| url |
http://hdl.handle.net/10451/46298 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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J Immunother Cancer. 2020 Sep;8(2):e001223 10.1136/jitc-2020-001223 2051-1426 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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BMJ Publishing Group Ltd. |
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BMJ Publishing Group Ltd. |
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