Functional purification of the monocarboxylate transporter of the yeast Candida utilis

Detalhes bibliográficos
Autor(a) principal: Baltazar, Fátima
Data de Publicação: 2006
Outros Autores: Cássio, Fernanda, Leão, Cecília
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/5680
Resumo: Plasma membranes of the yeast, Candida utilis, were solubilized with octyl-b-D- lucopyranoside and a fraction enriched in the lactate carrier was obtained with DEAE-Sepharose anion- exchange chromatography, after elution with 0.4 M NaCl. The uptake of lactic acid into proteoliposomes, containing the purified protein fraction and cytochrome c oxidase, was dependent on a proton-motive force and the transport specificity was consistent with the one of C. utilis intact cells. Overall, we have obtained a plasma membrane fraction enriched in the lactate carrier of C. utilis in which the transport properties were preserved. Given the similarities between the lactate transport of C. utilis and the one of mammalian cells, this purified system could be further explored to screen for specific lactate inhibitors, with potential therapeutic applications.
id RCAP_a94506b2e5a29ccfd6ad6744b03d7863
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/5680
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Functional purification of the monocarboxylate transporter of the yeast Candida utilisCandida utilisFunctional reconstitutionLactate carrierProtein purificationScience & TechnologyPlasma membranes of the yeast, Candida utilis, were solubilized with octyl-b-D- lucopyranoside and a fraction enriched in the lactate carrier was obtained with DEAE-Sepharose anion- exchange chromatography, after elution with 0.4 M NaCl. The uptake of lactic acid into proteoliposomes, containing the purified protein fraction and cytochrome c oxidase, was dependent on a proton-motive force and the transport specificity was consistent with the one of C. utilis intact cells. Overall, we have obtained a plasma membrane fraction enriched in the lactate carrier of C. utilis in which the transport properties were preserved. Given the similarities between the lactate transport of C. utilis and the one of mammalian cells, this purified system could be further explored to screen for specific lactate inhibitors, with potential therapeutic applications.SpringerUniversidade do MinhoBaltazar, FátimaCássio, FernandaLeão, Cecília20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/5680eng"Biotechnol lett". ISSN 0141-5492. 28:16 (Aug. 2006) 1221-1226.0141-549210.1007/s10529-006-9088-5168020970141-5492info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:50:54Zoai:repositorium.sdum.uminho.pt:1822/5680Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:49:40.066585Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Functional purification of the monocarboxylate transporter of the yeast Candida utilis
title Functional purification of the monocarboxylate transporter of the yeast Candida utilis
spellingShingle Functional purification of the monocarboxylate transporter of the yeast Candida utilis
Baltazar, Fátima
Candida utilis
Functional reconstitution
Lactate carrier
Protein purification
Science & Technology
title_short Functional purification of the monocarboxylate transporter of the yeast Candida utilis
title_full Functional purification of the monocarboxylate transporter of the yeast Candida utilis
title_fullStr Functional purification of the monocarboxylate transporter of the yeast Candida utilis
title_full_unstemmed Functional purification of the monocarboxylate transporter of the yeast Candida utilis
title_sort Functional purification of the monocarboxylate transporter of the yeast Candida utilis
author Baltazar, Fátima
author_facet Baltazar, Fátima
Cássio, Fernanda
Leão, Cecília
author_role author
author2 Cássio, Fernanda
Leão, Cecília
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Baltazar, Fátima
Cássio, Fernanda
Leão, Cecília
dc.subject.por.fl_str_mv Candida utilis
Functional reconstitution
Lactate carrier
Protein purification
Science & Technology
topic Candida utilis
Functional reconstitution
Lactate carrier
Protein purification
Science & Technology
description Plasma membranes of the yeast, Candida utilis, were solubilized with octyl-b-D- lucopyranoside and a fraction enriched in the lactate carrier was obtained with DEAE-Sepharose anion- exchange chromatography, after elution with 0.4 M NaCl. The uptake of lactic acid into proteoliposomes, containing the purified protein fraction and cytochrome c oxidase, was dependent on a proton-motive force and the transport specificity was consistent with the one of C. utilis intact cells. Overall, we have obtained a plasma membrane fraction enriched in the lactate carrier of C. utilis in which the transport properties were preserved. Given the similarities between the lactate transport of C. utilis and the one of mammalian cells, this purified system could be further explored to screen for specific lactate inhibitors, with potential therapeutic applications.
publishDate 2006
dc.date.none.fl_str_mv 2006
2006-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/5680
url http://hdl.handle.net/1822/5680
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Biotechnol lett". ISSN 0141-5492. 28:16 (Aug. 2006) 1221-1226.
0141-5492
10.1007/s10529-006-9088-5
16802097
0141-5492
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799133079776788480

Registros relacionados