Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis

Detalhes bibliográficos
Autor(a) principal: Feijão, Tália
Data de Publicação: 2022
Outros Autores: Marques, Bruno, Silva, Rui D., Carvalho, Célia, Sobral, Daniel, Matos, Ricardo, Tan, Tian, Pereira, António, Morais-de-Sá, Eurico, Maiato, Hélder, DeLuca, Steven Z., Martinho, Rui Gonçalo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/152211
Resumo: We are grateful to Torcato Martins for insightful scientific discussions. We also thank Cláudia Florindo for assistance in fluorescence microscopy, Margarida Neto for maintenance of Drosophila stock collection and technical support, and Alexandra Tavares for technical support. We acknowledge the TRiP collection at Harvard Medical School and BDSC for providing several of the Drosophila stocks used in this study. We acknowledge and thank Claudio E. Sunkel for antibodies. B.M. and R.D.S. are both supported by Portuguese national funding through Fundação para a Ciência e a Tecnologia (within the scope of the ProRegeM PhD program; PD/ 00117/2012, CRM:0027030) and DL 57/2016/CP1361/CT0019. The Light Microscopy Unit of ABC-RI was partially supported by Portuguese national funding (FCT: PPBI-POCI-01-0145-FEDER-022122). This work was developed with the support of the research infrastructure Congento (project LISBOA-01-0145-FEDER-022170). The Transgenic RNAi Project (TRiP) collection at Harvard Medical School was supported by NIH/NIGMS R01-GM084947. The funding bodies had no role in the design of this study; collection, analysis, and interpretation of data; and manuscript writing. Publisher Copyright: Copyright © 2022 the Author(s).
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spelling Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosismeiosisPolycomb group proteinspolycomplexessynaptonemal complextranscriptionGeneralWe are grateful to Torcato Martins for insightful scientific discussions. We also thank Cláudia Florindo for assistance in fluorescence microscopy, Margarida Neto for maintenance of Drosophila stock collection and technical support, and Alexandra Tavares for technical support. We acknowledge the TRiP collection at Harvard Medical School and BDSC for providing several of the Drosophila stocks used in this study. We acknowledge and thank Claudio E. Sunkel for antibodies. B.M. and R.D.S. are both supported by Portuguese national funding through Fundação para a Ciência e a Tecnologia (within the scope of the ProRegeM PhD program; PD/ 00117/2012, CRM:0027030) and DL 57/2016/CP1361/CT0019. The Light Microscopy Unit of ABC-RI was partially supported by Portuguese national funding (FCT: PPBI-POCI-01-0145-FEDER-022122). This work was developed with the support of the research infrastructure Congento (project LISBOA-01-0145-FEDER-022170). The Transgenic RNAi Project (TRiP) collection at Harvard Medical School was supported by NIH/NIGMS R01-GM084947. The funding bodies had no role in the design of this study; collection, analysis, and interpretation of data; and manuscript writing. Publisher Copyright: Copyright © 2022 the Author(s).The synaptonemal complex (SC) is a proteinaceous scaffold that is assembled between paired homologous chromosomes during the onset of meiosis. Timely expression of SC coding genes is essential for SC assembly and successful meiosis. However, SC components have an intrinsic tendency to self-organize into abnormal repetitive structures, which are not assembled between the paired homologs and whose formation is potentially deleterious for meiosis and gametogenesis. This creates an interesting conundrum, where SC genes need to be robustly expressed during meiosis, but their expression must be carefully regulated to prevent the formation of anomalous SC structures. In this manuscript, we show that the Polycomb group protein Sfmbt, the Drosophila ortholog of human MBTD1 and L3MBTL2, is required to avoid excessive expression of SC genes during prophase I. Although SC assembly is normal after Sfmbt depletion, SC disassembly is abnormal with the formation of multiple synaptonemal complexes (polycomplexes) within the oocyte. Overexpression of the SC gene corona and depletion of other Polycomb group proteins are similarly associated with polycomplex formation during SC disassembly. These polycomplexes are highly dynamic and have a well-defined periodic structure. Further confirming the importance of Sfmbt, germ line depletion of this protein is associated with significant metaphase I defects and a reduction in female fertility. Since transcription of SC genes mostly occurs during early prophase I, our results suggest a role of Sfmbt and other Polycomb group proteins in downregulating the expression of these and other early prophase I genes during later stages of meiosis.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNFeijão, TáliaMarques, BrunoSilva, Rui D.Carvalho, CéliaSobral, DanielMatos, RicardoTan, TianPereira, AntónioMorais-de-Sá, EuricoMaiato, HélderDeLuca, Steven Z.Martinho, Rui Gonçalo2023-04-27T22:18:53Z2022-10-182022-10-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/152211engFeijão, T., Marques, B., Silva, R. D., Carvalho, C., Sobral, D., Matos, R., Tan, T., Pereira, A., Morais-de-Sá, E., Maiato, H., DeLuca, S. Z., & Martinho, R. G. (2022). Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis. Proceedings of the National Academy of Sciences of the United States of America, 119(42), [e2204701119]. https://doi.org/10.1073/pnas.22047011190027-8424PURE: 56524273https://doi.org/10.1073/pnas.2204701119info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:34:33Zoai:run.unl.pt:10362/152211Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:54:49.877031Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis
title Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis
spellingShingle Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis
Feijão, Tália
meiosis
Polycomb group proteins
polycomplexes
synaptonemal complex
transcription
General
title_short Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis
title_full Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis
title_fullStr Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis
title_full_unstemmed Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis
title_sort Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis
author Feijão, Tália
author_facet Feijão, Tália
Marques, Bruno
Silva, Rui D.
Carvalho, Célia
Sobral, Daniel
Matos, Ricardo
Tan, Tian
Pereira, António
Morais-de-Sá, Eurico
Maiato, Hélder
DeLuca, Steven Z.
Martinho, Rui Gonçalo
author_role author
author2 Marques, Bruno
Silva, Rui D.
Carvalho, Célia
Sobral, Daniel
Matos, Ricardo
Tan, Tian
Pereira, António
Morais-de-Sá, Eurico
Maiato, Hélder
DeLuca, Steven Z.
Martinho, Rui Gonçalo
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Feijão, Tália
Marques, Bruno
Silva, Rui D.
Carvalho, Célia
Sobral, Daniel
Matos, Ricardo
Tan, Tian
Pereira, António
Morais-de-Sá, Eurico
Maiato, Hélder
DeLuca, Steven Z.
Martinho, Rui Gonçalo
dc.subject.por.fl_str_mv meiosis
Polycomb group proteins
polycomplexes
synaptonemal complex
transcription
General
topic meiosis
Polycomb group proteins
polycomplexes
synaptonemal complex
transcription
General
description We are grateful to Torcato Martins for insightful scientific discussions. We also thank Cláudia Florindo for assistance in fluorescence microscopy, Margarida Neto for maintenance of Drosophila stock collection and technical support, and Alexandra Tavares for technical support. We acknowledge the TRiP collection at Harvard Medical School and BDSC for providing several of the Drosophila stocks used in this study. We acknowledge and thank Claudio E. Sunkel for antibodies. B.M. and R.D.S. are both supported by Portuguese national funding through Fundação para a Ciência e a Tecnologia (within the scope of the ProRegeM PhD program; PD/ 00117/2012, CRM:0027030) and DL 57/2016/CP1361/CT0019. The Light Microscopy Unit of ABC-RI was partially supported by Portuguese national funding (FCT: PPBI-POCI-01-0145-FEDER-022122). This work was developed with the support of the research infrastructure Congento (project LISBOA-01-0145-FEDER-022170). The Transgenic RNAi Project (TRiP) collection at Harvard Medical School was supported by NIH/NIGMS R01-GM084947. The funding bodies had no role in the design of this study; collection, analysis, and interpretation of data; and manuscript writing. Publisher Copyright: Copyright © 2022 the Author(s).
publishDate 2022
dc.date.none.fl_str_mv 2022-10-18
2022-10-18T00:00:00Z
2023-04-27T22:18:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/152211
url http://hdl.handle.net/10362/152211
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Feijão, T., Marques, B., Silva, R. D., Carvalho, C., Sobral, D., Matos, R., Tan, T., Pereira, A., Morais-de-Sá, E., Maiato, H., DeLuca, S. Z., & Martinho, R. G. (2022). Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis. Proceedings of the National Academy of Sciences of the United States of America, 119(42), [e2204701119]. https://doi.org/10.1073/pnas.2204701119
0027-8424
PURE: 56524273
https://doi.org/10.1073/pnas.2204701119
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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