Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/54839 |
Resumo: | Copyright © 2022 the Author(s). Published by PNAS. This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND). |
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Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosisPolycomb group proteinsMeiosisPolycomplexesSynaptonemal complexTranscriptionCopyright © 2022 the Author(s). Published by PNAS. This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).The synaptonemal complex (SC) is a proteinaceous scaffold that is assembled between paired homologous chromosomes during the onset of meiosis. Timely expression of SC coding genes is essential for SC assembly and successful meiosis. However, SC components have an intrinsic tendency to self-organize into abnormal repetitive structures, which are not assembled between the paired homologs and whose formation is potentially deleterious for meiosis and gametogenesis. This creates an interesting conundrum, where SC genes need to be robustly expressed during meiosis, but their expression must be carefully regulated to prevent the formation of anomalous SC structures. In this manuscript, we show that the Polycomb group protein Sfmbt, the Drosophila ortholog of human MBTD1 and L3MBTL2, is required to avoid excessive expression of SC genes during prophase I. Although SC assembly is normal after Sfmbt depletion, SC disassembly is abnormal with the formation of multiple synaptonemal complexes (polycomplexes) within the oocyte. Overexpression of the SC gene corona and depletion of other Polycomb group proteins are similarly associated with polycomplex formation during SC disassembly. These polycomplexes are highly dynamic and have a well-defined periodic structure. Further confirming the importance of Sfmbt, germ line depletion of this protein is associated with significant metaphase I defects and a reduction in female fertility. Since transcription of SC genes mostly occurs during early prophase I, our results suggest a role of Sfmbt and other Polycomb group proteins in downregulating the expression of these and other early prophase I genes during later stages of meiosis.R.G.M. is supported by Portuguese national funding through Fundação para a Ciência e a Tecnologia (FCT grant refs. PTDC/BIA-BID/28441/2017 and PTDC/BIA-BID/1606/2020). B.M. and R.D.S. are both supported by Portuguese national funding through Fundação para a Ciência e a Tecnologia, respectively, PD/BD/128342/2017 (within the scope of the ProRegeM PhD program; PD/00117/2012, CRM:0027030) and DL 57/2016/CP1361/CT0019. The Light Microscopy Unit of ABC-RI was partially supported by Portuguese national funding (FCT: PPBI-POCI-01-0145-FEDER-022122). This work was developed with the support of the research infrastructure Congento (project LISBOA-01-0145-FEDER-022170). The Transgenic RNAi Project (TRiP) collection at Harvard Medical School was supported by NIH/NIGMS R01-GM084947.National Academy of ScienceRepositório da Universidade de LisboaFeijão, TáliaMarques, BrunoSilva, Rui D.Carvalho, CéliaSobral, DanielMatos, RicardoTan, TianPereira, AntónioMorais de Sá, EuricoMaiato, HélderDeLuca, Steven Z.Martinho, Rui Goncalo2022-10-20T14:42:05Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/54839engProc Natl Acad Sci USA . 2022 Oct 18;119(42):e22047011190027-842410.1073/pnas.22047011191091-6490info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T18:17:13Zoai:repositorio.ul.pt:10451/54839Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T18:17:13Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis |
title |
Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis |
spellingShingle |
Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis Feijão, Tália Polycomb group proteins Meiosis Polycomplexes Synaptonemal complex Transcription |
title_short |
Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis |
title_full |
Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis |
title_fullStr |
Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis |
title_full_unstemmed |
Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis |
title_sort |
Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis |
author |
Feijão, Tália |
author_facet |
Feijão, Tália Marques, Bruno Silva, Rui D. Carvalho, Célia Sobral, Daniel Matos, Ricardo Tan, Tian Pereira, António Morais de Sá, Eurico Maiato, Hélder DeLuca, Steven Z. Martinho, Rui Goncalo |
author_role |
author |
author2 |
Marques, Bruno Silva, Rui D. Carvalho, Célia Sobral, Daniel Matos, Ricardo Tan, Tian Pereira, António Morais de Sá, Eurico Maiato, Hélder DeLuca, Steven Z. Martinho, Rui Goncalo |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Feijão, Tália Marques, Bruno Silva, Rui D. Carvalho, Célia Sobral, Daniel Matos, Ricardo Tan, Tian Pereira, António Morais de Sá, Eurico Maiato, Hélder DeLuca, Steven Z. Martinho, Rui Goncalo |
dc.subject.por.fl_str_mv |
Polycomb group proteins Meiosis Polycomplexes Synaptonemal complex Transcription |
topic |
Polycomb group proteins Meiosis Polycomplexes Synaptonemal complex Transcription |
description |
Copyright © 2022 the Author(s). Published by PNAS. This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND). |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-20T14:42:05Z 2022 2022-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/54839 |
url |
http://hdl.handle.net/10451/54839 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Proc Natl Acad Sci USA . 2022 Oct 18;119(42):e2204701119 0027-8424 10.1073/pnas.2204701119 1091-6490 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
National Academy of Science |
publisher.none.fl_str_mv |
National Academy of Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817549206957588480 |