Metabolite Profiling of Human Amniotic Fluid by Hyphenated Nuclear Magnetic Resonance Spectroscopy

Detalhes bibliográficos
Autor(a) principal: Graça, Gonçalo
Data de Publicação: 2008
Outros Autores: Duarte, Iola F., Goodfellow, Brian J., Carreira, Isabel M., Couceiro, Ana Bela, Domingues, Maria do Rosário, Spraul, Manfred, Tseng, Li-Hong, Gil, Ana M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/10562
https://doi.org/10.1021/ac800907f
Resumo: The metabolic profiling of human amniotic fluid (HAF) is of potential interest for the diagnosis of disorders in the mother or the fetus. In order to build a comprehensive metabolite database for HAF, hyphenated NMR has been used, for the first time, for systematic HAF profiling. Experiments were carried out using reverse-phase (RP) and ion-exchange liquid chromatography (LC), in order to detect less and more polar compounds, respectively. RP-LC conditions achieved good separation of amino acids, some sugars, and xanthines. Subsequent NMR and MS analysis enabled the rapid identification of 30 compounds, including 3-methyl-2-oxovalerate and 4-aminohippurate identified in HAF for the first time, to our knowledge. Under ion-exchange LC conditions, a different set of 30 compounds was detected, including sugars, organic acids, several derivatives of organic acids, and amino acids. In this experiment, five compounds were identified for the first time in HAF: d-xylitol, amino acid derivatives (N-acetylalanine, N-acetylglycine, 2-oxoleucine), and isovalerate. The nonendogenous nature of some metabolites (caffeine, paraxanthine, d-xylitol, sorbitol) is discussed. Hyphenated NMR has allowed the rapid detection of 60 metabolites in HAF, some of which are not detectable by standard NMR due to low abundance (μM) and signal overlap thus enabling an extended metabolite database to be built for HAF.
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spelling Metabolite Profiling of Human Amniotic Fluid by Hyphenated Nuclear Magnetic Resonance SpectroscopyThe metabolic profiling of human amniotic fluid (HAF) is of potential interest for the diagnosis of disorders in the mother or the fetus. In order to build a comprehensive metabolite database for HAF, hyphenated NMR has been used, for the first time, for systematic HAF profiling. Experiments were carried out using reverse-phase (RP) and ion-exchange liquid chromatography (LC), in order to detect less and more polar compounds, respectively. RP-LC conditions achieved good separation of amino acids, some sugars, and xanthines. Subsequent NMR and MS analysis enabled the rapid identification of 30 compounds, including 3-methyl-2-oxovalerate and 4-aminohippurate identified in HAF for the first time, to our knowledge. Under ion-exchange LC conditions, a different set of 30 compounds was detected, including sugars, organic acids, several derivatives of organic acids, and amino acids. In this experiment, five compounds were identified for the first time in HAF: d-xylitol, amino acid derivatives (N-acetylalanine, N-acetylglycine, 2-oxoleucine), and isovalerate. The nonendogenous nature of some metabolites (caffeine, paraxanthine, d-xylitol, sorbitol) is discussed. Hyphenated NMR has allowed the rapid detection of 60 metabolites in HAF, some of which are not detectable by standard NMR due to low abundance (μM) and signal overlap thus enabling an extended metabolite database to be built for HAF.American Chemical Society2008-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/10562http://hdl.handle.net/10316/10562https://doi.org/10.1021/ac800907fengAnalytical Chemistry. 80:15 (2008) 6085-60920003-2700Graça, GonçaloDuarte, Iola F.Goodfellow, Brian J.Carreira, Isabel M.Couceiro, Ana BelaDomingues, Maria do RosárioSpraul, ManfredTseng, Li-HongGil, Ana M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2019-12-10T12:05:17Zoai:estudogeral.uc.pt:10316/10562Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:38.101177Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Metabolite Profiling of Human Amniotic Fluid by Hyphenated Nuclear Magnetic Resonance Spectroscopy
title Metabolite Profiling of Human Amniotic Fluid by Hyphenated Nuclear Magnetic Resonance Spectroscopy
spellingShingle Metabolite Profiling of Human Amniotic Fluid by Hyphenated Nuclear Magnetic Resonance Spectroscopy
Graça, Gonçalo
title_short Metabolite Profiling of Human Amniotic Fluid by Hyphenated Nuclear Magnetic Resonance Spectroscopy
title_full Metabolite Profiling of Human Amniotic Fluid by Hyphenated Nuclear Magnetic Resonance Spectroscopy
title_fullStr Metabolite Profiling of Human Amniotic Fluid by Hyphenated Nuclear Magnetic Resonance Spectroscopy
title_full_unstemmed Metabolite Profiling of Human Amniotic Fluid by Hyphenated Nuclear Magnetic Resonance Spectroscopy
title_sort Metabolite Profiling of Human Amniotic Fluid by Hyphenated Nuclear Magnetic Resonance Spectroscopy
author Graça, Gonçalo
author_facet Graça, Gonçalo
Duarte, Iola F.
Goodfellow, Brian J.
Carreira, Isabel M.
Couceiro, Ana Bela
Domingues, Maria do Rosário
Spraul, Manfred
Tseng, Li-Hong
Gil, Ana M.
author_role author
author2 Duarte, Iola F.
Goodfellow, Brian J.
Carreira, Isabel M.
Couceiro, Ana Bela
Domingues, Maria do Rosário
Spraul, Manfred
Tseng, Li-Hong
Gil, Ana M.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Graça, Gonçalo
Duarte, Iola F.
Goodfellow, Brian J.
Carreira, Isabel M.
Couceiro, Ana Bela
Domingues, Maria do Rosário
Spraul, Manfred
Tseng, Li-Hong
Gil, Ana M.
description The metabolic profiling of human amniotic fluid (HAF) is of potential interest for the diagnosis of disorders in the mother or the fetus. In order to build a comprehensive metabolite database for HAF, hyphenated NMR has been used, for the first time, for systematic HAF profiling. Experiments were carried out using reverse-phase (RP) and ion-exchange liquid chromatography (LC), in order to detect less and more polar compounds, respectively. RP-LC conditions achieved good separation of amino acids, some sugars, and xanthines. Subsequent NMR and MS analysis enabled the rapid identification of 30 compounds, including 3-methyl-2-oxovalerate and 4-aminohippurate identified in HAF for the first time, to our knowledge. Under ion-exchange LC conditions, a different set of 30 compounds was detected, including sugars, organic acids, several derivatives of organic acids, and amino acids. In this experiment, five compounds were identified for the first time in HAF: d-xylitol, amino acid derivatives (N-acetylalanine, N-acetylglycine, 2-oxoleucine), and isovalerate. The nonendogenous nature of some metabolites (caffeine, paraxanthine, d-xylitol, sorbitol) is discussed. Hyphenated NMR has allowed the rapid detection of 60 metabolites in HAF, some of which are not detectable by standard NMR due to low abundance (μM) and signal overlap thus enabling an extended metabolite database to be built for HAF.
publishDate 2008
dc.date.none.fl_str_mv 2008-08-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/10562
http://hdl.handle.net/10316/10562
https://doi.org/10.1021/ac800907f
url http://hdl.handle.net/10316/10562
https://doi.org/10.1021/ac800907f
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Analytical Chemistry. 80:15 (2008) 6085-6092
0003-2700
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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