Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line

Detalhes bibliográficos
Autor(a) principal: Luís, Carla
Data de Publicação: 2019
Outros Autores: Castaño-Guerrero, Yuselis, Soares, Raquel, Sales, Goreti, Fernandes, Rúben
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/18737
Resumo: Doxorubicin (DOXO) is an adjuvant chemotherapy agent and is also commonly used in cell biology research. Cytotoxic assays in cell culture are frequently used in order to stablish drug concentrations that are useful for controlling cell proliferation. One common cytotoxic method used is 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT). Our present research aims to support future studies in engaging MTT assay using DOXO that exhibits a strong red coloration and fluorescence, and so it is assumed that DOXO may interfere with commonly used colorimetric assays such as MTT. The interference of DOXO in the MTT determination was evaluated in a Breast Cancer cell line Michigan Cancer Foundation-7 (MCF-7). The interference was evaluated by means of spectroscopic methods in particular spectrophometry and fluorescence spectroscopy of MTT and DOXO. We postulate that the medium and the MTT reagent itself can interfere on the metabolic activity method, so in order to achieve better results, DMEM was replaced by a neutral buffer like Phosphate-buffered saline (PBS). This protocol may be extremely useful in future studies involving DOXO.
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spelling Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell lineMTTDoxorubicinChemotherapyBreast cancerMCF-7Doxorubicin (DOXO) is an adjuvant chemotherapy agent and is also commonly used in cell biology research. Cytotoxic assays in cell culture are frequently used in order to stablish drug concentrations that are useful for controlling cell proliferation. One common cytotoxic method used is 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT). Our present research aims to support future studies in engaging MTT assay using DOXO that exhibits a strong red coloration and fluorescence, and so it is assumed that DOXO may interfere with commonly used colorimetric assays such as MTT. The interference of DOXO in the MTT determination was evaluated in a Breast Cancer cell line Michigan Cancer Foundation-7 (MCF-7). The interference was evaluated by means of spectroscopic methods in particular spectrophometry and fluorescence spectroscopy of MTT and DOXO. We postulate that the medium and the MTT reagent itself can interfere on the metabolic activity method, so in order to achieve better results, DMEM was replaced by a neutral buffer like Phosphate-buffered saline (PBS). This protocol may be extremely useful in future studies involving DOXO.MDPIRepositório Científico do Instituto Politécnico do PortoLuís, CarlaCastaño-Guerrero, YuselisSoares, RaquelSales, GoretiFernandes, Rúben2021-10-19T13:49:03Z2019-042019-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/18737engLuís, C., Castaño-Guerrero, Y., Soares, R., Sales, G., & Fernandes, R. (2019). Avoiding the Interference of Doxorubicin with MTT Measurements on the MCF-7 Breast Cancer Cell Line. Methods and Protocols, 2(2), 29. https://www.mdpi.com/2409-9279/2/2/292409-927910.3390/mps2020029info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:11:28Zoai:recipp.ipp.pt:10400.22/18737Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:38:48.200480Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line
title Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line
spellingShingle Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line
Luís, Carla
MTT
Doxorubicin
Chemotherapy
Breast cancer
MCF-7
title_short Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line
title_full Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line
title_fullStr Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line
title_full_unstemmed Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line
title_sort Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line
author Luís, Carla
author_facet Luís, Carla
Castaño-Guerrero, Yuselis
Soares, Raquel
Sales, Goreti
Fernandes, Rúben
author_role author
author2 Castaño-Guerrero, Yuselis
Soares, Raquel
Sales, Goreti
Fernandes, Rúben
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Luís, Carla
Castaño-Guerrero, Yuselis
Soares, Raquel
Sales, Goreti
Fernandes, Rúben
dc.subject.por.fl_str_mv MTT
Doxorubicin
Chemotherapy
Breast cancer
MCF-7
topic MTT
Doxorubicin
Chemotherapy
Breast cancer
MCF-7
description Doxorubicin (DOXO) is an adjuvant chemotherapy agent and is also commonly used in cell biology research. Cytotoxic assays in cell culture are frequently used in order to stablish drug concentrations that are useful for controlling cell proliferation. One common cytotoxic method used is 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT). Our present research aims to support future studies in engaging MTT assay using DOXO that exhibits a strong red coloration and fluorescence, and so it is assumed that DOXO may interfere with commonly used colorimetric assays such as MTT. The interference of DOXO in the MTT determination was evaluated in a Breast Cancer cell line Michigan Cancer Foundation-7 (MCF-7). The interference was evaluated by means of spectroscopic methods in particular spectrophometry and fluorescence spectroscopy of MTT and DOXO. We postulate that the medium and the MTT reagent itself can interfere on the metabolic activity method, so in order to achieve better results, DMEM was replaced by a neutral buffer like Phosphate-buffered saline (PBS). This protocol may be extremely useful in future studies involving DOXO.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
2019-04-01T00:00:00Z
2021-10-19T13:49:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/18737
url http://hdl.handle.net/10400.22/18737
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Luís, C., Castaño-Guerrero, Y., Soares, R., Sales, G., & Fernandes, R. (2019). Avoiding the Interference of Doxorubicin with MTT Measurements on the MCF-7 Breast Cancer Cell Line. Methods and Protocols, 2(2), 29. https://www.mdpi.com/2409-9279/2/2/29
2409-9279
10.3390/mps2020029
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dc.publisher.none.fl_str_mv MDPI
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