Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/18737 |
Resumo: | Doxorubicin (DOXO) is an adjuvant chemotherapy agent and is also commonly used in cell biology research. Cytotoxic assays in cell culture are frequently used in order to stablish drug concentrations that are useful for controlling cell proliferation. One common cytotoxic method used is 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT). Our present research aims to support future studies in engaging MTT assay using DOXO that exhibits a strong red coloration and fluorescence, and so it is assumed that DOXO may interfere with commonly used colorimetric assays such as MTT. The interference of DOXO in the MTT determination was evaluated in a Breast Cancer cell line Michigan Cancer Foundation-7 (MCF-7). The interference was evaluated by means of spectroscopic methods in particular spectrophometry and fluorescence spectroscopy of MTT and DOXO. We postulate that the medium and the MTT reagent itself can interfere on the metabolic activity method, so in order to achieve better results, DMEM was replaced by a neutral buffer like Phosphate-buffered saline (PBS). This protocol may be extremely useful in future studies involving DOXO. |
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Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell lineMTTDoxorubicinChemotherapyBreast cancerMCF-7Doxorubicin (DOXO) is an adjuvant chemotherapy agent and is also commonly used in cell biology research. Cytotoxic assays in cell culture are frequently used in order to stablish drug concentrations that are useful for controlling cell proliferation. One common cytotoxic method used is 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT). Our present research aims to support future studies in engaging MTT assay using DOXO that exhibits a strong red coloration and fluorescence, and so it is assumed that DOXO may interfere with commonly used colorimetric assays such as MTT. The interference of DOXO in the MTT determination was evaluated in a Breast Cancer cell line Michigan Cancer Foundation-7 (MCF-7). The interference was evaluated by means of spectroscopic methods in particular spectrophometry and fluorescence spectroscopy of MTT and DOXO. We postulate that the medium and the MTT reagent itself can interfere on the metabolic activity method, so in order to achieve better results, DMEM was replaced by a neutral buffer like Phosphate-buffered saline (PBS). This protocol may be extremely useful in future studies involving DOXO.MDPIRepositório Científico do Instituto Politécnico do PortoLuís, CarlaCastaño-Guerrero, YuselisSoares, RaquelSales, GoretiFernandes, Rúben2021-10-19T13:49:03Z2019-042019-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/18737engLuís, C., Castaño-Guerrero, Y., Soares, R., Sales, G., & Fernandes, R. (2019). Avoiding the Interference of Doxorubicin with MTT Measurements on the MCF-7 Breast Cancer Cell Line. Methods and Protocols, 2(2), 29. https://www.mdpi.com/2409-9279/2/2/292409-927910.3390/mps2020029info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:11:28Zoai:recipp.ipp.pt:10400.22/18737Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:38:48.200480Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line |
title |
Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line |
spellingShingle |
Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line Luís, Carla MTT Doxorubicin Chemotherapy Breast cancer MCF-7 |
title_short |
Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line |
title_full |
Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line |
title_fullStr |
Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line |
title_full_unstemmed |
Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line |
title_sort |
Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line |
author |
Luís, Carla |
author_facet |
Luís, Carla Castaño-Guerrero, Yuselis Soares, Raquel Sales, Goreti Fernandes, Rúben |
author_role |
author |
author2 |
Castaño-Guerrero, Yuselis Soares, Raquel Sales, Goreti Fernandes, Rúben |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Luís, Carla Castaño-Guerrero, Yuselis Soares, Raquel Sales, Goreti Fernandes, Rúben |
dc.subject.por.fl_str_mv |
MTT Doxorubicin Chemotherapy Breast cancer MCF-7 |
topic |
MTT Doxorubicin Chemotherapy Breast cancer MCF-7 |
description |
Doxorubicin (DOXO) is an adjuvant chemotherapy agent and is also commonly used in cell biology research. Cytotoxic assays in cell culture are frequently used in order to stablish drug concentrations that are useful for controlling cell proliferation. One common cytotoxic method used is 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT). Our present research aims to support future studies in engaging MTT assay using DOXO that exhibits a strong red coloration and fluorescence, and so it is assumed that DOXO may interfere with commonly used colorimetric assays such as MTT. The interference of DOXO in the MTT determination was evaluated in a Breast Cancer cell line Michigan Cancer Foundation-7 (MCF-7). The interference was evaluated by means of spectroscopic methods in particular spectrophometry and fluorescence spectroscopy of MTT and DOXO. We postulate that the medium and the MTT reagent itself can interfere on the metabolic activity method, so in order to achieve better results, DMEM was replaced by a neutral buffer like Phosphate-buffered saline (PBS). This protocol may be extremely useful in future studies involving DOXO. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-04 2019-04-01T00:00:00Z 2021-10-19T13:49:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/18737 |
url |
http://hdl.handle.net/10400.22/18737 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Luís, C., Castaño-Guerrero, Y., Soares, R., Sales, G., & Fernandes, R. (2019). Avoiding the Interference of Doxorubicin with MTT Measurements on the MCF-7 Breast Cancer Cell Line. Methods and Protocols, 2(2), 29. https://www.mdpi.com/2409-9279/2/2/29 2409-9279 10.3390/mps2020029 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131475965116416 |