Genetic Diversity and Antibiotic Resistance Among Coagulase-Negative Staphylococci Recovered from Birds of Prey in Portugal

Detalhes bibliográficos
Autor(a) principal: Sousa, Margarida
Data de Publicação: 2016
Outros Autores: Silva, Nuno, Igrejas, Gilberto, Sargo, Roberto, Benito, Daniel, Gómez, Paula, Lozano, Carmen, Manageiro, Vera, Torres, Carmen, Caniça, Manuela, Poeta, Patrícia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/4243
Resumo: Wild animal populations in contact with antimicrobials and antimicrobial resistant bacteria that are daily released into the environment are able to become unintentional hosts of these resistant microorganisms. To clarify this issue, our study evaluated the presence of antibiotic resistance determinants on coagulase-negative staphylococci recovered from birds of prey and studied their genetic relatedness by pulsed-field gel electrophoresis (PFGE). The unusual vga(A) and erm(T) genes, which confer resistance to clindamycin and erythromycin, respectively, were detected in Staphylococcus sciuri or Staphylococcus xylosus strains and the tet(K) gene in Staphylococcus kloosii. The PFGE patterns showed that three S. xylosus (isolated of Strix aluco and Otus scops) and two S. sciuri (recovered from Strix aluco and Milvus migrans) were clonally indistinguishable. These animals could be a source of unusual antimicrobial resistance determinants for highly used antibiotics in veterinary clinical practice.
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spelling Genetic Diversity and Antibiotic Resistance Among Coagulase-Negative Staphylococci Recovered from Birds of Prey in PortugalStaphylococcus spp.Antimicrobial Resistant BacteriaPFGEPortugalBirds of PreyCoagulase-negative StaphylococciResistência aos AntimicrobianosWild animal populations in contact with antimicrobials and antimicrobial resistant bacteria that are daily released into the environment are able to become unintentional hosts of these resistant microorganisms. To clarify this issue, our study evaluated the presence of antibiotic resistance determinants on coagulase-negative staphylococci recovered from birds of prey and studied their genetic relatedness by pulsed-field gel electrophoresis (PFGE). The unusual vga(A) and erm(T) genes, which confer resistance to clindamycin and erythromycin, respectively, were detected in Staphylococcus sciuri or Staphylococcus xylosus strains and the tet(K) gene in Staphylococcus kloosii. The PFGE patterns showed that three S. xylosus (isolated of Strix aluco and Otus scops) and two S. sciuri (recovered from Strix aluco and Milvus migrans) were clonally indistinguishable. These animals could be a source of unusual antimicrobial resistance determinants for highly used antibiotics in veterinary clinical practice.This work was supported by the Unidade de Ciências Biomoleculares Aplicadas-UCIBIO, which is financed by national funds from FCT/MEC (UID/Multi/04378/2013) and cofinanced by the ERDF under the PT2020 Partnership Agreement (POCI-01–0145-FEDER-007728). Margarida Andrade Sousa has her Ph.D. fellowship granted by FCT (Fundacção para a Ciência e a Tecnologia, Portugal) with the reference SFRH/BD/87302/2012; Vera Manageiro has her Pos-Doc fellowship granted by FCT with the reference SFRH/BPD/77486/2011, both cofinanced by the Social European Union Fund and the Operational Program for Human Potential and National Board for Reference and Strategic Programs (POPH/QREN), Portugal. Nuno Silva was supported by ‘‘Programa Cieˆncia 2008’’ cofinanced by POPH/QREN Type 4.2: Employment Promotion Scientific subsidized by the European Social Fund and National Funds of the Ministry of Science and Technology for Higher Education (MCTES). Paula Go´mez has a Ph.D. fellowship from the Universidad de La Rioja (Spain). Part of this work was supported by Project SAF2012-35474 from the Ministerio de Economı´a y Competitividad of Spain and the Fondo Europeo de Desarrollo Regional (FEDER).Mary Ann LiebertRepositório Científico do Instituto Nacional de SaúdeSousa, MargaridaSilva, NunoIgrejas, GilbertoSargo, RobertoBenito, DanielGómez, PaulaLozano, CarmenManageiro, VeraTorres, CarmenCaniça, ManuelaPoeta, Patrícia2017-03-19T01:30:11Z2016-122016-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/4243engMicrob Drug Resist. 2016 Dec;22(8):727-730. Epub 2016 Mar 181076-629410.1089/mdr.2015.0266info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:40:18Zoai:repositorio.insa.pt:10400.18/4243Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:39:08.308906Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genetic Diversity and Antibiotic Resistance Among Coagulase-Negative Staphylococci Recovered from Birds of Prey in Portugal
title Genetic Diversity and Antibiotic Resistance Among Coagulase-Negative Staphylococci Recovered from Birds of Prey in Portugal
spellingShingle Genetic Diversity and Antibiotic Resistance Among Coagulase-Negative Staphylococci Recovered from Birds of Prey in Portugal
Sousa, Margarida
Staphylococcus spp.
Antimicrobial Resistant Bacteria
PFGE
Portugal
Birds of Prey
Coagulase-negative Staphylococci
Resistência aos Antimicrobianos
title_short Genetic Diversity and Antibiotic Resistance Among Coagulase-Negative Staphylococci Recovered from Birds of Prey in Portugal
title_full Genetic Diversity and Antibiotic Resistance Among Coagulase-Negative Staphylococci Recovered from Birds of Prey in Portugal
title_fullStr Genetic Diversity and Antibiotic Resistance Among Coagulase-Negative Staphylococci Recovered from Birds of Prey in Portugal
title_full_unstemmed Genetic Diversity and Antibiotic Resistance Among Coagulase-Negative Staphylococci Recovered from Birds of Prey in Portugal
title_sort Genetic Diversity and Antibiotic Resistance Among Coagulase-Negative Staphylococci Recovered from Birds of Prey in Portugal
author Sousa, Margarida
author_facet Sousa, Margarida
Silva, Nuno
Igrejas, Gilberto
Sargo, Roberto
Benito, Daniel
Gómez, Paula
Lozano, Carmen
Manageiro, Vera
Torres, Carmen
Caniça, Manuela
Poeta, Patrícia
author_role author
author2 Silva, Nuno
Igrejas, Gilberto
Sargo, Roberto
Benito, Daniel
Gómez, Paula
Lozano, Carmen
Manageiro, Vera
Torres, Carmen
Caniça, Manuela
Poeta, Patrícia
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Sousa, Margarida
Silva, Nuno
Igrejas, Gilberto
Sargo, Roberto
Benito, Daniel
Gómez, Paula
Lozano, Carmen
Manageiro, Vera
Torres, Carmen
Caniça, Manuela
Poeta, Patrícia
dc.subject.por.fl_str_mv Staphylococcus spp.
Antimicrobial Resistant Bacteria
PFGE
Portugal
Birds of Prey
Coagulase-negative Staphylococci
Resistência aos Antimicrobianos
topic Staphylococcus spp.
Antimicrobial Resistant Bacteria
PFGE
Portugal
Birds of Prey
Coagulase-negative Staphylococci
Resistência aos Antimicrobianos
description Wild animal populations in contact with antimicrobials and antimicrobial resistant bacteria that are daily released into the environment are able to become unintentional hosts of these resistant microorganisms. To clarify this issue, our study evaluated the presence of antibiotic resistance determinants on coagulase-negative staphylococci recovered from birds of prey and studied their genetic relatedness by pulsed-field gel electrophoresis (PFGE). The unusual vga(A) and erm(T) genes, which confer resistance to clindamycin and erythromycin, respectively, were detected in Staphylococcus sciuri or Staphylococcus xylosus strains and the tet(K) gene in Staphylococcus kloosii. The PFGE patterns showed that three S. xylosus (isolated of Strix aluco and Otus scops) and two S. sciuri (recovered from Strix aluco and Milvus migrans) were clonally indistinguishable. These animals could be a source of unusual antimicrobial resistance determinants for highly used antibiotics in veterinary clinical practice.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
2016-12-01T00:00:00Z
2017-03-19T01:30:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/4243
url http://hdl.handle.net/10400.18/4243
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microb Drug Resist. 2016 Dec;22(8):727-730. Epub 2016 Mar 18
1076-6294
10.1089/mdr.2015.0266
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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