Coagulase-negative staphylococci clones are widely distributed in the hospital and community
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/pathogens10070792 http://hdl.handle.net/11449/229473 |
Resumo: | Coagulase-negative staphylococci (CoNS) may be considered contaminants when isolated from clinical specimens but may also be a cause of true infection. This study aimed to compare the clonality and SCCmec type of a collection of CoNS isolated from blood cultures of inpatients, nasal swabs of healthy individuals, and patients with chronic wounds, all from the same community, using SCCmec typing, pulsed-field gel electrophoresis (PFGE), and MLST. Staphylococcus epidermidis, exhibited high clonal diversity, but hospital and community clusters were observed. Nosocomial S. epidermidis clones belonged to sequence types ST2, ST6, and ST23. Some Staphylococcus haemolyticus clones were found to circulate in the hospital and community, while Staphylococcus saprophyticus exhibited very high clonal diversity. Staphylococcus lugdunensis, Staphylococcus warneri, and Staphylococcus capitis revealed several isolates belonging to the same clone in the hospital and community. The detection of different SCCmec types within the same cluster indicated high diversity. S. epidermidis was associated with SCCmec I and III, S. haemolyticus with I and II, S. capitis with type V, Staphylococcus hominis with mec complex type A and ccr1, and S. warneri and S. saprophyticus with SCCmec I. The generation of elements and new combinations of cassette genes were highly associated with CoNS isolates, suggesting that SCCmec may not be a good marker of clonality in these bacteria. |
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Coagulase-negative staphylococci clones are widely distributed in the hospital and communityCoagulase-negative staphylococciMLSTPFGESCCmecCoagulase-negative staphylococci (CoNS) may be considered contaminants when isolated from clinical specimens but may also be a cause of true infection. This study aimed to compare the clonality and SCCmec type of a collection of CoNS isolated from blood cultures of inpatients, nasal swabs of healthy individuals, and patients with chronic wounds, all from the same community, using SCCmec typing, pulsed-field gel electrophoresis (PFGE), and MLST. Staphylococcus epidermidis, exhibited high clonal diversity, but hospital and community clusters were observed. Nosocomial S. epidermidis clones belonged to sequence types ST2, ST6, and ST23. Some Staphylococcus haemolyticus clones were found to circulate in the hospital and community, while Staphylococcus saprophyticus exhibited very high clonal diversity. Staphylococcus lugdunensis, Staphylococcus warneri, and Staphylococcus capitis revealed several isolates belonging to the same clone in the hospital and community. The detection of different SCCmec types within the same cluster indicated high diversity. S. epidermidis was associated with SCCmec I and III, S. haemolyticus with I and II, S. capitis with type V, Staphylococcus hominis with mec complex type A and ccr1, and S. warneri and S. saprophyticus with SCCmec I. The generation of elements and new combinations of cassette genes were highly associated with CoNS isolates, suggesting that SCCmec may not be a good marker of clonality in these bacteria.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Chemical and Biological Sciences Microbiology and Immunology Sector Institute of Biosciences of Botucatu Universidade Estadual Paulista-UNESPDepartment of Anatomic Pathology Instituto Lauro de Souza LimaDepartment of Chemical and Biological Sciences Microbiology and Immunology Sector Institute of Biosciences of Botucatu Universidade Estadual Paulista-UNESPCNPq: 303603/2020-8Universidade Estadual Paulista (UNESP)Instituto Lauro de Souza LimaPinheiro-Hubinger, Luiza [UNESP]Riboli, Danilo Flávio Moraes [UNESP]Abraão, Lígia Maria [UNESP]Pereira Franchi, Eliane Patricia Lino [UNESP]Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP]2022-04-29T08:32:42Z2022-04-29T08:32:42Z2021-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/pathogens10070792Pathogens, v. 10, n. 7, 2021.2076-0817http://hdl.handle.net/11449/22947310.3390/pathogens100707922-s2.0-85114385390Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPathogensinfo:eu-repo/semantics/openAccess2022-04-29T08:32:42Zoai:repositorio.unesp.br:11449/229473Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:56:37.496445Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Coagulase-negative staphylococci clones are widely distributed in the hospital and community |
title |
Coagulase-negative staphylococci clones are widely distributed in the hospital and community |
spellingShingle |
Coagulase-negative staphylococci clones are widely distributed in the hospital and community Pinheiro-Hubinger, Luiza [UNESP] Coagulase-negative staphylococci MLST PFGE SCCmec |
title_short |
Coagulase-negative staphylococci clones are widely distributed in the hospital and community |
title_full |
Coagulase-negative staphylococci clones are widely distributed in the hospital and community |
title_fullStr |
Coagulase-negative staphylococci clones are widely distributed in the hospital and community |
title_full_unstemmed |
Coagulase-negative staphylococci clones are widely distributed in the hospital and community |
title_sort |
Coagulase-negative staphylococci clones are widely distributed in the hospital and community |
author |
Pinheiro-Hubinger, Luiza [UNESP] |
author_facet |
Pinheiro-Hubinger, Luiza [UNESP] Riboli, Danilo Flávio Moraes [UNESP] Abraão, Lígia Maria [UNESP] Pereira Franchi, Eliane Patricia Lino [UNESP] Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP] |
author_role |
author |
author2 |
Riboli, Danilo Flávio Moraes [UNESP] Abraão, Lígia Maria [UNESP] Pereira Franchi, Eliane Patricia Lino [UNESP] Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Instituto Lauro de Souza Lima |
dc.contributor.author.fl_str_mv |
Pinheiro-Hubinger, Luiza [UNESP] Riboli, Danilo Flávio Moraes [UNESP] Abraão, Lígia Maria [UNESP] Pereira Franchi, Eliane Patricia Lino [UNESP] Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP] |
dc.subject.por.fl_str_mv |
Coagulase-negative staphylococci MLST PFGE SCCmec |
topic |
Coagulase-negative staphylococci MLST PFGE SCCmec |
description |
Coagulase-negative staphylococci (CoNS) may be considered contaminants when isolated from clinical specimens but may also be a cause of true infection. This study aimed to compare the clonality and SCCmec type of a collection of CoNS isolated from blood cultures of inpatients, nasal swabs of healthy individuals, and patients with chronic wounds, all from the same community, using SCCmec typing, pulsed-field gel electrophoresis (PFGE), and MLST. Staphylococcus epidermidis, exhibited high clonal diversity, but hospital and community clusters were observed. Nosocomial S. epidermidis clones belonged to sequence types ST2, ST6, and ST23. Some Staphylococcus haemolyticus clones were found to circulate in the hospital and community, while Staphylococcus saprophyticus exhibited very high clonal diversity. Staphylococcus lugdunensis, Staphylococcus warneri, and Staphylococcus capitis revealed several isolates belonging to the same clone in the hospital and community. The detection of different SCCmec types within the same cluster indicated high diversity. S. epidermidis was associated with SCCmec I and III, S. haemolyticus with I and II, S. capitis with type V, Staphylococcus hominis with mec complex type A and ccr1, and S. warneri and S. saprophyticus with SCCmec I. The generation of elements and new combinations of cassette genes were highly associated with CoNS isolates, suggesting that SCCmec may not be a good marker of clonality in these bacteria. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-01 2022-04-29T08:32:42Z 2022-04-29T08:32:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/pathogens10070792 Pathogens, v. 10, n. 7, 2021. 2076-0817 http://hdl.handle.net/11449/229473 10.3390/pathogens10070792 2-s2.0-85114385390 |
url |
http://dx.doi.org/10.3390/pathogens10070792 http://hdl.handle.net/11449/229473 |
identifier_str_mv |
Pathogens, v. 10, n. 7, 2021. 2076-0817 10.3390/pathogens10070792 2-s2.0-85114385390 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pathogens |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129376533872640 |