Coagulase-negative staphylococci clones are widely distributed in the hospital and community

Detalhes bibliográficos
Autor(a) principal: Pinheiro-Hubinger, Luiza [UNESP]
Data de Publicação: 2021
Outros Autores: Riboli, Danilo Flávio Moraes [UNESP], Abraão, Lígia Maria [UNESP], Pereira Franchi, Eliane Patricia Lino [UNESP], Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/pathogens10070792
http://hdl.handle.net/11449/229473
Resumo: Coagulase-negative staphylococci (CoNS) may be considered contaminants when isolated from clinical specimens but may also be a cause of true infection. This study aimed to compare the clonality and SCCmec type of a collection of CoNS isolated from blood cultures of inpatients, nasal swabs of healthy individuals, and patients with chronic wounds, all from the same community, using SCCmec typing, pulsed-field gel electrophoresis (PFGE), and MLST. Staphylococcus epidermidis, exhibited high clonal diversity, but hospital and community clusters were observed. Nosocomial S. epidermidis clones belonged to sequence types ST2, ST6, and ST23. Some Staphylococcus haemolyticus clones were found to circulate in the hospital and community, while Staphylococcus saprophyticus exhibited very high clonal diversity. Staphylococcus lugdunensis, Staphylococcus warneri, and Staphylococcus capitis revealed several isolates belonging to the same clone in the hospital and community. The detection of different SCCmec types within the same cluster indicated high diversity. S. epidermidis was associated with SCCmec I and III, S. haemolyticus with I and II, S. capitis with type V, Staphylococcus hominis with mec complex type A and ccr1, and S. warneri and S. saprophyticus with SCCmec I. The generation of elements and new combinations of cassette genes were highly associated with CoNS isolates, suggesting that SCCmec may not be a good marker of clonality in these bacteria.
id UNSP_07460e3d0e3f13d005de912b14bb4eec
oai_identifier_str oai:repositorio.unesp.br:11449/229473
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Coagulase-negative staphylococci clones are widely distributed in the hospital and communityCoagulase-negative staphylococciMLSTPFGESCCmecCoagulase-negative staphylococci (CoNS) may be considered contaminants when isolated from clinical specimens but may also be a cause of true infection. This study aimed to compare the clonality and SCCmec type of a collection of CoNS isolated from blood cultures of inpatients, nasal swabs of healthy individuals, and patients with chronic wounds, all from the same community, using SCCmec typing, pulsed-field gel electrophoresis (PFGE), and MLST. Staphylococcus epidermidis, exhibited high clonal diversity, but hospital and community clusters were observed. Nosocomial S. epidermidis clones belonged to sequence types ST2, ST6, and ST23. Some Staphylococcus haemolyticus clones were found to circulate in the hospital and community, while Staphylococcus saprophyticus exhibited very high clonal diversity. Staphylococcus lugdunensis, Staphylococcus warneri, and Staphylococcus capitis revealed several isolates belonging to the same clone in the hospital and community. The detection of different SCCmec types within the same cluster indicated high diversity. S. epidermidis was associated with SCCmec I and III, S. haemolyticus with I and II, S. capitis with type V, Staphylococcus hominis with mec complex type A and ccr1, and S. warneri and S. saprophyticus with SCCmec I. The generation of elements and new combinations of cassette genes were highly associated with CoNS isolates, suggesting that SCCmec may not be a good marker of clonality in these bacteria.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Chemical and Biological Sciences Microbiology and Immunology Sector Institute of Biosciences of Botucatu Universidade Estadual Paulista-UNESPDepartment of Anatomic Pathology Instituto Lauro de Souza LimaDepartment of Chemical and Biological Sciences Microbiology and Immunology Sector Institute of Biosciences of Botucatu Universidade Estadual Paulista-UNESPCNPq: 303603/2020-8Universidade Estadual Paulista (UNESP)Instituto Lauro de Souza LimaPinheiro-Hubinger, Luiza [UNESP]Riboli, Danilo Flávio Moraes [UNESP]Abraão, Lígia Maria [UNESP]Pereira Franchi, Eliane Patricia Lino [UNESP]Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP]2022-04-29T08:32:42Z2022-04-29T08:32:42Z2021-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/pathogens10070792Pathogens, v. 10, n. 7, 2021.2076-0817http://hdl.handle.net/11449/22947310.3390/pathogens100707922-s2.0-85114385390Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPathogensinfo:eu-repo/semantics/openAccess2022-04-29T08:32:42Zoai:repositorio.unesp.br:11449/229473Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:32:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Coagulase-negative staphylococci clones are widely distributed in the hospital and community
title Coagulase-negative staphylococci clones are widely distributed in the hospital and community
spellingShingle Coagulase-negative staphylococci clones are widely distributed in the hospital and community
Pinheiro-Hubinger, Luiza [UNESP]
Coagulase-negative staphylococci
MLST
PFGE
SCCmec
title_short Coagulase-negative staphylococci clones are widely distributed in the hospital and community
title_full Coagulase-negative staphylococci clones are widely distributed in the hospital and community
title_fullStr Coagulase-negative staphylococci clones are widely distributed in the hospital and community
title_full_unstemmed Coagulase-negative staphylococci clones are widely distributed in the hospital and community
title_sort Coagulase-negative staphylococci clones are widely distributed in the hospital and community
author Pinheiro-Hubinger, Luiza [UNESP]
author_facet Pinheiro-Hubinger, Luiza [UNESP]
Riboli, Danilo Flávio Moraes [UNESP]
Abraão, Lígia Maria [UNESP]
Pereira Franchi, Eliane Patricia Lino [UNESP]
Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP]
author_role author
author2 Riboli, Danilo Flávio Moraes [UNESP]
Abraão, Lígia Maria [UNESP]
Pereira Franchi, Eliane Patricia Lino [UNESP]
Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Instituto Lauro de Souza Lima
dc.contributor.author.fl_str_mv Pinheiro-Hubinger, Luiza [UNESP]
Riboli, Danilo Flávio Moraes [UNESP]
Abraão, Lígia Maria [UNESP]
Pereira Franchi, Eliane Patricia Lino [UNESP]
Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP]
dc.subject.por.fl_str_mv Coagulase-negative staphylococci
MLST
PFGE
SCCmec
topic Coagulase-negative staphylococci
MLST
PFGE
SCCmec
description Coagulase-negative staphylococci (CoNS) may be considered contaminants when isolated from clinical specimens but may also be a cause of true infection. This study aimed to compare the clonality and SCCmec type of a collection of CoNS isolated from blood cultures of inpatients, nasal swabs of healthy individuals, and patients with chronic wounds, all from the same community, using SCCmec typing, pulsed-field gel electrophoresis (PFGE), and MLST. Staphylococcus epidermidis, exhibited high clonal diversity, but hospital and community clusters were observed. Nosocomial S. epidermidis clones belonged to sequence types ST2, ST6, and ST23. Some Staphylococcus haemolyticus clones were found to circulate in the hospital and community, while Staphylococcus saprophyticus exhibited very high clonal diversity. Staphylococcus lugdunensis, Staphylococcus warneri, and Staphylococcus capitis revealed several isolates belonging to the same clone in the hospital and community. The detection of different SCCmec types within the same cluster indicated high diversity. S. epidermidis was associated with SCCmec I and III, S. haemolyticus with I and II, S. capitis with type V, Staphylococcus hominis with mec complex type A and ccr1, and S. warneri and S. saprophyticus with SCCmec I. The generation of elements and new combinations of cassette genes were highly associated with CoNS isolates, suggesting that SCCmec may not be a good marker of clonality in these bacteria.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-01
2022-04-29T08:32:42Z
2022-04-29T08:32:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/pathogens10070792
Pathogens, v. 10, n. 7, 2021.
2076-0817
http://hdl.handle.net/11449/229473
10.3390/pathogens10070792
2-s2.0-85114385390
url http://dx.doi.org/10.3390/pathogens10070792
http://hdl.handle.net/11449/229473
identifier_str_mv Pathogens, v. 10, n. 7, 2021.
2076-0817
10.3390/pathogens10070792
2-s2.0-85114385390
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pathogens
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799965490645303296