Controlling bacteriological contamination of environmental surfaces at the biological isolation and containment unit of a veterinary teaching hospital

Detalhes bibliográficos
Autor(a) principal: Verdial, C
Data de Publicação: 2021
Outros Autores: Carneiro, Carla, Machado, Inês, Tavares, Luis, Almeida, Virgilio, Oliveira, Manuela, Gil, Solange
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/22457
Resumo: Research Areas: Veterinary Sciences
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spelling Controlling bacteriological contamination of environmental surfaces at the biological isolation and containment unit of a veterinary teaching hospitalNosocomial infectionSurveillanceBacteriological controlHospital environmentResearch Areas: Veterinary SciencesBackground: The Biological Isolation and Containment Unit (BICU) is a subunit of the Teaching Hospital of the Faculty of Veterinary Medicine of the University of Lisbon, Portugal, for the admission of animals with confirmed infectious diseases or under clinical suspicion and waiting for a diagnosis. As a high-risk environment for the transmission of infectious agents, it is extremely important to implement programs for the surveillance of nosocomial microorganisms in these facilities. The purpose of this study was to evaluate the level of bacterial contamination of the BICU environmental surfaces and to implement corrective actions on disinfection protocols. Swab samples were collected from selected environmental surfaces in 3 different areas of the BICU (isolation, work, and preparatory rooms) to evaluate the total aerobic bacterial load and investigate the presence of 4 nosocomial microorganisms: vancomycin-resistant Enterococcus spp., methicillin-resistant Staphylococcus aureus, 3rd-generation cephalosporin-resistant Escherichia coli, and carbapenem-resistant Pseudomonas aeruginosa. Bacterial quantification was performed by using non-selective media, while specific selective media were used for the isolation of the target microorganisms. Isolates were identified based on their macro and microscopic characteristics and their biochemical profile. Subsequently, new disinfection protocols were implemented, and their effectiveness evaluated. Results: The surfaces with the highest bacterial load in the isolation, preparatory, and worker’s rooms were the cages, hand-held sponge, and telephone, respectively. Regarding the 4 pathogens investigated, Enterococcus spp. were the most frequently isolated (11.3%), followed by E. coli (1.5%) and P. aeruginosa (1.5%). One of the P. aeruginosa isolates obtained was resistant to imipenem. In the end, new disinfection protocols were implemented, which proved to be effective in reducing bacterial counts by 99.99% in cages and the sponge, and by 90 to 99% on the telephone. Conclusions: This study allows to conclude that the cages and the human contact surfaces were the most contaminated in the isolation rooms. Nevertheless, the new disinfection strategies seemed to be effective in reducing environmental contamination, including by some potentially nosocomial agents, although more samples must be analyzed for definitive conclusions. These results may contribute to highlight the importance of infection prevention and control measures, as fundamental tools to reduce the spread of infectious agents in the hospital environment.BMCRepositório da Universidade de LisboaVerdial, CCarneiro, CarlaMachado, InêsTavares, LuisAlmeida, VirgilioOliveira, ManuelaGil, Solange2021-11-01T18:44:46Z2021-06-282021-06-28T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22457engVerdial C, Carneiro C, Machado I, Tavares L, Almeida V, Oliveira M, Gil S. 2021. Controlling bacteriological contamination of environmental surfaces at the biological isolation and containment unit of a veterinary teaching hospital. Irish Veterinary Journal 74(1):18. DOI:10.1186/s13620-021-00197-z0368-076210.1186/s13620-021-00197-z2046-0481info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:51:59Zoai:www.repository.utl.pt:10400.5/22457Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:52.709616Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Controlling bacteriological contamination of environmental surfaces at the biological isolation and containment unit of a veterinary teaching hospital
title Controlling bacteriological contamination of environmental surfaces at the biological isolation and containment unit of a veterinary teaching hospital
spellingShingle Controlling bacteriological contamination of environmental surfaces at the biological isolation and containment unit of a veterinary teaching hospital
Verdial, C
Nosocomial infection
Surveillance
Bacteriological control
Hospital environment
title_short Controlling bacteriological contamination of environmental surfaces at the biological isolation and containment unit of a veterinary teaching hospital
title_full Controlling bacteriological contamination of environmental surfaces at the biological isolation and containment unit of a veterinary teaching hospital
title_fullStr Controlling bacteriological contamination of environmental surfaces at the biological isolation and containment unit of a veterinary teaching hospital
title_full_unstemmed Controlling bacteriological contamination of environmental surfaces at the biological isolation and containment unit of a veterinary teaching hospital
title_sort Controlling bacteriological contamination of environmental surfaces at the biological isolation and containment unit of a veterinary teaching hospital
author Verdial, C
author_facet Verdial, C
Carneiro, Carla
Machado, Inês
Tavares, Luis
Almeida, Virgilio
Oliveira, Manuela
Gil, Solange
author_role author
author2 Carneiro, Carla
Machado, Inês
Tavares, Luis
Almeida, Virgilio
Oliveira, Manuela
Gil, Solange
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Verdial, C
Carneiro, Carla
Machado, Inês
Tavares, Luis
Almeida, Virgilio
Oliveira, Manuela
Gil, Solange
dc.subject.por.fl_str_mv Nosocomial infection
Surveillance
Bacteriological control
Hospital environment
topic Nosocomial infection
Surveillance
Bacteriological control
Hospital environment
description Research Areas: Veterinary Sciences
publishDate 2021
dc.date.none.fl_str_mv 2021-11-01T18:44:46Z
2021-06-28
2021-06-28T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/22457
url http://hdl.handle.net/10400.5/22457
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Verdial C, Carneiro C, Machado I, Tavares L, Almeida V, Oliveira M, Gil S. 2021. Controlling bacteriological contamination of environmental surfaces at the biological isolation and containment unit of a veterinary teaching hospital. Irish Veterinary Journal 74(1):18. DOI:10.1186/s13620-021-00197-z
0368-0762
10.1186/s13620-021-00197-z
2046-0481
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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