Interlaboratory validation of a multiplex qPCR method for the detection of listeria monocytogenes in a ready-to-eat seafood product
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/83902 |
Resumo: | Listeria monocytogenes is a major foodborne pathogen which mainly infects susceptible individuals through the consumption of contaminated foods. To this end, ready-to-eat (RTE) food products are of particular concern as this microorganism is widely distributed, can survive, and even grow, under adverse conditions, and thus must be carefully controlled. In the present study, an interlaboratory ring trial was organized to evaluate an open formula qPCR-based method for the detection of L. monocytogenes. The molecular method was evaluated on a novel RTE seafood product, developed in the framework of a European project, the SEAFOODAGE (EAPA_758/2018). Six laboratories located in Spain and Portugal participated in the study, and the results obtained indicated that this new method presented high diagnostic sensitivity (100%) reaching a low limit of detection (<10CFU/25 g) with an overall agreement with the reference method, attending to the Cohen's k, of 0.97 that is interpreted as almost complete agreement. |
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Interlaboratory validation of a multiplex qPCR method for the detection of listeria monocytogenes in a ready-to-eat seafood productInterlaboratory validationqPCRReady-to-eatFish productsAlternative methodsListeria monocytogenesScience & TechnologyListeria monocytogenes is a major foodborne pathogen which mainly infects susceptible individuals through the consumption of contaminated foods. To this end, ready-to-eat (RTE) food products are of particular concern as this microorganism is widely distributed, can survive, and even grow, under adverse conditions, and thus must be carefully controlled. In the present study, an interlaboratory ring trial was organized to evaluate an open formula qPCR-based method for the detection of L. monocytogenes. The molecular method was evaluated on a novel RTE seafood product, developed in the framework of a European project, the SEAFOODAGE (EAPA_758/2018). Six laboratories located in Spain and Portugal participated in the study, and the results obtained indicated that this new method presented high diagnostic sensitivity (100%) reaching a low limit of detection (<10CFU/25 g) with an overall agreement with the reference method, attending to the Cohen's k, of 0.97 that is interpreted as almost complete agreement.This work was financially supported by the Seafood Age project, which was co-financed by the Interreg Atlantic Area Program (EAPA_758/2018) though the European Development Fund (ERDF). Mrs. Sarah Azinheiro was financed by a Ph.D. grant from the Fundação para a Ciência e a Tecnologia (SFRH/BD/140396/2018). Dr. Alejandro Garrido-Maestu and Luís D. R. Melo acknowledge funding from the Fundação para a Ciência e Tecnologia through the Scientific Employment Stimulus Program (2021.02810.CEECIND and 2021.00221.CEECIND, respectively). This study was supported by the Fundação para a Ciência e a Tecnologia (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoAzinheiro, SarahRodríguez-López, PedroLozano-León, AntonioGuedes, HugoRegal, PatriciaFranco, Carlos M.Cepeda, AlbertoTeixeira, Maria Pilar AraújoMelo, Luís Daniel RodriguesSilva, DanielaFernández, AnaFaria, MárciaRoumani, FoteiniJuan Herrera,Prado, MartaLópez-Cabo, MartaGarrido-Maestu, Alejandro2023-082023-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/83902engAzinheiro, Sarah; Rodríguez-López, Pedro; Lozano-León, Antonio; Guedes, Hugo; Regal, Patricia; Franco, Carlos M.; Cepeda, Alberto; Teixeira, Pilar; Melo, Luís Daniel Rodrigues; Silva, Daniela; Fernández, Ana; Faria, Márcia; Roumani, Foteini; Herrera, Juan; Prado, Marta; López-Cabo, Marta; Garrido-Maestu, Alejandro, Interlaboratory validation of a multiplex qPCR method for the detection of Listeria monocytogenes in a ready-to-eat seafood product. Food Control, 150(109769), 20230956-713510.1016/j.foodcont.2023.109769http://www.journals.elsevier.com/food-controlinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:45:39Zoai:repositorium.sdum.uminho.pt:1822/83902Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:43:31.700331Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Interlaboratory validation of a multiplex qPCR method for the detection of listeria monocytogenes in a ready-to-eat seafood product |
title |
Interlaboratory validation of a multiplex qPCR method for the detection of listeria monocytogenes in a ready-to-eat seafood product |
spellingShingle |
Interlaboratory validation of a multiplex qPCR method for the detection of listeria monocytogenes in a ready-to-eat seafood product Azinheiro, Sarah Interlaboratory validation qPCR Ready-to-eat Fish products Alternative methods Listeria monocytogenes Science & Technology |
title_short |
Interlaboratory validation of a multiplex qPCR method for the detection of listeria monocytogenes in a ready-to-eat seafood product |
title_full |
Interlaboratory validation of a multiplex qPCR method for the detection of listeria monocytogenes in a ready-to-eat seafood product |
title_fullStr |
Interlaboratory validation of a multiplex qPCR method for the detection of listeria monocytogenes in a ready-to-eat seafood product |
title_full_unstemmed |
Interlaboratory validation of a multiplex qPCR method for the detection of listeria monocytogenes in a ready-to-eat seafood product |
title_sort |
Interlaboratory validation of a multiplex qPCR method for the detection of listeria monocytogenes in a ready-to-eat seafood product |
author |
Azinheiro, Sarah |
author_facet |
Azinheiro, Sarah Rodríguez-López, Pedro Lozano-León, Antonio Guedes, Hugo Regal, Patricia Franco, Carlos M. Cepeda, Alberto Teixeira, Maria Pilar Araújo Melo, Luís Daniel Rodrigues Silva, Daniela Fernández, Ana Faria, Márcia Roumani, Foteini Juan Herrera, Prado, Marta López-Cabo, Marta Garrido-Maestu, Alejandro |
author_role |
author |
author2 |
Rodríguez-López, Pedro Lozano-León, Antonio Guedes, Hugo Regal, Patricia Franco, Carlos M. Cepeda, Alberto Teixeira, Maria Pilar Araújo Melo, Luís Daniel Rodrigues Silva, Daniela Fernández, Ana Faria, Márcia Roumani, Foteini Juan Herrera, Prado, Marta López-Cabo, Marta Garrido-Maestu, Alejandro |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Azinheiro, Sarah Rodríguez-López, Pedro Lozano-León, Antonio Guedes, Hugo Regal, Patricia Franco, Carlos M. Cepeda, Alberto Teixeira, Maria Pilar Araújo Melo, Luís Daniel Rodrigues Silva, Daniela Fernández, Ana Faria, Márcia Roumani, Foteini Juan Herrera, Prado, Marta López-Cabo, Marta Garrido-Maestu, Alejandro |
dc.subject.por.fl_str_mv |
Interlaboratory validation qPCR Ready-to-eat Fish products Alternative methods Listeria monocytogenes Science & Technology |
topic |
Interlaboratory validation qPCR Ready-to-eat Fish products Alternative methods Listeria monocytogenes Science & Technology |
description |
Listeria monocytogenes is a major foodborne pathogen which mainly infects susceptible individuals through the consumption of contaminated foods. To this end, ready-to-eat (RTE) food products are of particular concern as this microorganism is widely distributed, can survive, and even grow, under adverse conditions, and thus must be carefully controlled. In the present study, an interlaboratory ring trial was organized to evaluate an open formula qPCR-based method for the detection of L. monocytogenes. The molecular method was evaluated on a novel RTE seafood product, developed in the framework of a European project, the SEAFOODAGE (EAPA_758/2018). Six laboratories located in Spain and Portugal participated in the study, and the results obtained indicated that this new method presented high diagnostic sensitivity (100%) reaching a low limit of detection (<10CFU/25 g) with an overall agreement with the reference method, attending to the Cohen's k, of 0.97 that is interpreted as almost complete agreement. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-08 2023-08-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/83902 |
url |
https://hdl.handle.net/1822/83902 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Azinheiro, Sarah; Rodríguez-López, Pedro; Lozano-León, Antonio; Guedes, Hugo; Regal, Patricia; Franco, Carlos M.; Cepeda, Alberto; Teixeira, Pilar; Melo, Luís Daniel Rodrigues; Silva, Daniela; Fernández, Ana; Faria, Márcia; Roumani, Foteini; Herrera, Juan; Prado, Marta; López-Cabo, Marta; Garrido-Maestu, Alejandro, Interlaboratory validation of a multiplex qPCR method for the detection of Listeria monocytogenes in a ready-to-eat seafood product. Food Control, 150(109769), 2023 0956-7135 10.1016/j.foodcont.2023.109769 http://www.journals.elsevier.com/food-control |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132992187138048 |