Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples

Detalhes bibliográficos
Autor(a) principal: Pacífico, Cátia
Data de Publicação: 2019
Outros Autores: Hilbert, Miriam, Sofka, Dmitrij, Dinhopl, Nora, Pap, Ildiko-Julia, Aspöck, Christoph, Carrico, Joao Andre, Hilbert, Friederike
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/46406
Resumo: Copyright © 2019 Pacífico, Hilbert, Sofka, Dinhopl, Pap, Aspöck, Carriço and Hilbert. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
id RCAP_ac4b8b579c1826f3189432f37d9b363d
oai_identifier_str oai:repositorio.ul.pt:10451/46406
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samplesBacteriophagesUrineTracheal aspiratesBloodE. coliPeduovirusTunavirinaeTequintavirusCopyright © 2019 Pacífico, Hilbert, Sofka, Dinhopl, Pap, Aspöck, Carriço and Hilbert. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.The interaction between bacteriophages, bacteria and the human host as a tripartite system has recently captured attention. The taxonomic diversity of bacteriophages, as a natural parasite of bacteria, still remains obscure in human body biomes, representing a so-called "viral dark matter." Here, we isolated and characterized coliphages from blood, urine and tracheal aspirates samples collected at a tertiary care hospital in Austria. Phages were more often isolated from blood, followed by urine and tracheal aspirates. Phylogenetic analysis and genome comparisons allowed the identification of phages belonging to the Tunavirinae subfamily, and to the Peduovirus and Tequintavirus genera. Tunavirinae phages cluster together and are found in samples from 14 patients, suggesting their prevalence across a variety of human samples. When compared with other phage genomes, the highest similarity level was at 87.69% average nucleotide identity (ANI), which suggests that these are in fact a newly isolated phage species. Tequintavirus phages share a 95.90% with phage 3_29, challenging the ANI threshold currently accepted to differentiate phage species. The isolated phages appear to be virulent, with the exception of the Peduovirus members, which are integrative and seem to reside as prophages in bacterial genomes.FrontiersRepositório da Universidade de LisboaPacífico, CátiaHilbert, MiriamSofka, DmitrijDinhopl, NoraPap, Ildiko-JuliaAspöck, ChristophCarrico, Joao AndreHilbert, Friederike2021-02-17T17:03:15Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/46406engFront. Microbiol. 10:248410.3389/fmicb.2019.024841664-302Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:48:40Zoai:repositorio.ul.pt:10451/46406Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:58:35.122865Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples
title Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples
spellingShingle Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples
Pacífico, Cátia
Bacteriophages
Urine
Tracheal aspirates
Blood
E. coli
Peduovirus
Tunavirinae
Tequintavirus
title_short Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples
title_full Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples
title_fullStr Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples
title_full_unstemmed Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples
title_sort Natural occurrence of Escherichia coli-infecting bacteriophages in clinical samples
author Pacífico, Cátia
author_facet Pacífico, Cátia
Hilbert, Miriam
Sofka, Dmitrij
Dinhopl, Nora
Pap, Ildiko-Julia
Aspöck, Christoph
Carrico, Joao Andre
Hilbert, Friederike
author_role author
author2 Hilbert, Miriam
Sofka, Dmitrij
Dinhopl, Nora
Pap, Ildiko-Julia
Aspöck, Christoph
Carrico, Joao Andre
Hilbert, Friederike
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Pacífico, Cátia
Hilbert, Miriam
Sofka, Dmitrij
Dinhopl, Nora
Pap, Ildiko-Julia
Aspöck, Christoph
Carrico, Joao Andre
Hilbert, Friederike
dc.subject.por.fl_str_mv Bacteriophages
Urine
Tracheal aspirates
Blood
E. coli
Peduovirus
Tunavirinae
Tequintavirus
topic Bacteriophages
Urine
Tracheal aspirates
Blood
E. coli
Peduovirus
Tunavirinae
Tequintavirus
description Copyright © 2019 Pacífico, Hilbert, Sofka, Dinhopl, Pap, Aspöck, Carriço and Hilbert. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01T00:00:00Z
2021-02-17T17:03:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/46406
url http://hdl.handle.net/10451/46406
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Front. Microbiol. 10:2484
10.3389/fmicb.2019.02484
1664-302X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799134531758850048

Registros relacionados